Strain number NIES-824  
Phylum Cyanophyta  
Class Cyanophyceae  
Scientific name Dolichospermum smithii (Komárek) Wacklin, Hoffmann & Komárek  
Synonym Anabaena solitaria f. smithii Komarek 1958; Anabaena smithii (Komárek) M.Watanabe 1992  
Former name Anabaena smithii (Komárek) M.Watanabe  
Common name Blue-green alga ; Cyanobacteria  
Locality (Date of collection) Hasse River, Ogasawara, Tokyo, Japan (1998-03-**)  
Latitude / Longitude  
Habitat (Isolation source) Freshwater (Lake water)  
History < Li, Renhui  
Isolator (Date of isolation) Li, Renhui (1998-03-**)  
Identified by Li, Renhui  
State of strain Subculture; Unialgal; Clonal; Non-axenic  
Culture condition
(Preculture condition)
Medium:  CT  
Temperature:  20 C
Light intensity:  9-15 µmol photons/m2/sec, L/D cycle:  10L:14D
Duration:  2 M  
Gene information 16S rRNA ( LC319756 )  
Cell size (min - max) 8 - 15 μm  
Organization Filamentous 
Characteristics Cyanobacterial water bloom (aoko) ; Offensive odor ; geosmin production (Matsushita et al. 2010)  
Other strain no. Other strain no. : Ana Ha 1  
Miao, H., Zhang, J., Shen, Q., Ichise, S., Asada, Y., Tian, Y., Utsumi, M., Lei, Z., Zhang, Z., Takanashi, H., Fujimoto, N., Nishimura, O., Akiba, M., Shimizu, K. 2022 Development of rapid PCR methods for the detection and quantification of geosmin-producing Dolichospermum spp. Water Air Soil Pollut., 233, 394 (article ID).
Keywords: Cyanobacteria; Musty odor; Geosmin; geoA gene; Whole-cell PCR; Whole-cell qPCR
Strain(s): 82416581660 
DOI: 10.1007/s11270-022-05859-4

Shen, O., Wang, Q., Miao, H., Shimada, M., Utsumi, M., Lei, Z., Zhang, Z., Nishimura, O., Asada, Y., Fujimoto, N., Takanashi, H., Akiba, M., Shimizu, K. 2021 Temperature affects growth, geosmin/2-methylisoborneol production, and gene expression in two cyanobacterial species. Environ. Sci. Pollut. Res., 29, 12017–12026.
Keywords: Cyanobacteria; Temperature; Cell growth; Geosmin; 2-MIB; Gene expression
Strain(s): 512824 
PubMed: 34558048
DOI: 10.1007/s11356-021-16593-5

Kim, K., Yoon, Y., Cho, H., Hwang, S. J 2020 Molecular probes to evaluate the synthesis and production potential of an odorous compound (2-methylisoborneol) in cyanobacteria. Int. J. Environ. Res. Public Health, 17, 1933 (article ID).
Keywords: 2-Methylisoborneol (2-MIB); cyanobacteria; molecular probes; mibC; real-time PCR
Strain(s): 19512824 
PubMed: 32188031
DOI: 10.3390/ijerph17061933

Shen, Q., Shimizu, K., Miao, H., Tsukino, S., Utsumi, M., Lei, Z., Zhang, Z., Nishimura, O., Asada, Y., Fujimoto, N., Takanashi, H., Akiba, M. 2020 Effects of elevated nitrogen on the growth and geosmin productivity of Dolichospermum smithii. Environ. Sci. Pollut. Res., 28, 177–184.
Keywords: Geosmin; geoA Expression; Cyanobacteria; Nitrogen; Earthy-musty odor
Strain(s): 824 
PubMed: 32803599
DOI: 10.1007/s11356-020-10429-4

Chiu, Y.-T., Yen, H.-K., Lin, T.-F. 2016 An alternativemethodtoquantify2-MIB producing cyanobacteriain in drinking water reservoirs: Method development and field applications. Environ. Res., 151, 618-627.
Keywords: Cyanobacteria; 2-methylisoborneol(2-MIB); 2-MIB-synthesis gene(mibC gene); Tasteandodor; Real-timePCR
Strain(s): 8151282490521042116 
PubMed: 27607443
DOI: 10.1016/j.envres.2016.08.034

Matsushita, T., Kobayashi, Y., Nakamura, K., Matsui, Y., Ohno, K. 2010 Effect of transmembrane pressure on geosmin release from Cyanobacterial cell during microfiltration. Water Practice & Technology, 5, wpt2010049 (article ID).
Keywords: Cyanobacteria; geosmin; microfiltration; water treatment
Strain(s): 824 
DOI: 10.2166/wpt.2010.049

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