Strain number NIES-797  
Phylum Chlorophyta  
Class Chlorophyceae  
Scientific name Desmodesmus subspicatus (Chodat) E.Hegewald & A.W.F.Schmidt  
Synonym Scenedesmus subspicatus Chodat  
Former name  
Common name Green alga  
Locality (Date of collection) inside NIES, Onogawa, Tsukuba, Ibaraki, Japan (1992-06-17)  
Latitude / Longitude  
Habitat (Isolation source) Freshwater (Pond water)  
History < Kasai, Fumie  
Isolator (Date of isolation) Kasai, Fumie (1992-07-01)  
Identified by Nakano, Taketo; Confirmed by DNA sequencing at NIES  
State of strain Cryopreservation; Unialgal; Clonal; Non-axenic  
Culture condition
(Preculture condition)
Medium:  C  
Temperature:  20 (25) C
Light intensity:  2 (14-16) µmol photons/m2/sec, L/D cycle:  10L:14D
Duration:  6 M (21 D)  
Gene information  
Cell size (min - max) 8 - 13 μm  
Characteristics Test strain (Nagai, T., 2016) ; Herbicide (simetryn) susceptible  
Other strain no. Other strain no. : B8-7  
Remarks Cryopreserved 
Xing, H., Li, Q., Zhao, Y., Gao, H., Li, L., Zhang, Y., Yu, X. 2023 Exogenous proline boosts the co-accumulation of astaxanthin and biomass in stress-induced Haematococcus pluvialis. Bioresour. Technol., 369, 128488 (article ID).
Keywords: Haematococcus pluvialis; Proline; Biomass; Astaxanthin productivity; Abiotic stresses
Strain(s): 144712797 
PubMed: 36528181
DOI: 10.1016/j.biortech.2022.128488

Gu, Y., Tobino, T., Nakajima, F. 2021 Determining the relative importance of dietborne and waterborne toxicity of 4-tert-butylphenol and 4-tert-octylphenol to the benthic crustacean, Heterocypris incongruens. Environ. Sci. Technol., 55, 7939–7948.
Keywords: alkylphenol; toxicity test; dietborne toxicity; exposure route; Heterocypris incongruens; ISO 14371
Strain(s): 947972170 
PubMed: 34047553
DOI: 10.1021/acs.est.0c08164

Nagai, T. 2017 Predicting herbicide mixture effects on multiple algal species using mixture toxicity models. Environ. Toxicol. Chem., 36, 2624-2630.
Keywords: Pesticide; Species sensitivity distribution; Mixture toxicology; Algae; Mode of action
Strain(s): 71487512797 
PubMed: 28316094
DOI: 10.1002/etc.3800

Nagai, T., Taya, K., Yoda, I. 2016 Comparative toxicity of 20 herbicides to 5 periphytic algae and the relationship with mode of action. Environ. Toxicol. Chem., 35, 368-375.
Keywords: Attached algae; In-vivo fluorescence; Pesticides; Species sensitivity distribution
Strain(s): 71487512797 
PubMed: 26174500
DOI: 10.1002/etc.3150

Nagai, T. 2014 河川付着藻類を用いた農薬の毒性試験マニュアル Ver. 1.0 In Research Project for Environmental Risk Assessment of Nutrients and Organochemicals, NIAES, 52 pp (in Japanese).
Strain(s): 71487512797 

Nagai, T., Taya, K., Annoh, H., Ishihara, S. 2013 Application of a fluorometric microplate algal toxicity assay for riverine periphytic algal species. Ecotox. Environ. Safe., 94, 37-44.
Keywords: Attached algae; In-vivo fluorescence; Pesticides; Species sensitivity distribution
Strain(s): 357173414487512797 
DOI: 10.1016/j.ecoenv.2013.04.020

Kasai, F. & Hanazato, T. 1995 Genetic changes in phytoplankton communities exposed to the herbicide simetryn in outdoor experimental ponds. Arch. Environ. Contam. Toxicol., 28, 154-160.
Strain(s): 797798799800801802 
DOI: 10.1007/BF00217610

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