Strain number NIES-733  
Phylum Chlorophyta  
Class Chlorophyceae  
Scientific name Volvox carteri F.Stein f. kawasakiensis Nozaki  
Former name  
Common name Green alga ; Volvocine alga  
Locality (Date of collection) Kawasaki, Kanagawa, Japan (1984-01-**)  
Latitude / Longitude 35.544547 / 139.655969 
Habitat (Isolation source) Freshwater (Paddy soil)  
History < Nozaki, Hisayoshi  
Isolator (Date of isolation) Nozaki, Hisayoshi (1984-02-25)  
Identified by Nozaki, Hisayoshi  
State of strain Subculture; Unialgal; Clonal; Axenic[2018 Feb]  
Culture condition
(Preculture condition)
Medium:  VT  
Temperature:  23 C
Light intensity:  21-29 µmol photons/m2/sec, L/D cycle:  10L:14D
Duration:  42 D  
Gene information 18S-ITS1-5.8S-ITS2-28S ( LC004707 ) , mat3 ( GU735448 AB771944 ) , PRP4 ( GU735457 ) , PRX1 ( GU735466 )  
Cell size (min - max)  
Organization Colonial; Flagellate 
Characteristics Phototaxis ; Authentic strain ; Heterothallic ; Dioecious ; Oogamy ; Male ; Crosses with NIES-732  
Other strain no. Other strain no. : KK-5  
Remarks Fragile species to transportation stresses; Read and agree "How to order 4.1"; Axenic 
Kawafune K., Hongoh, Y., Hamaji, T ., Sakamoto, T., Kurata, T., Hirooka, S., Miyagishima, S., Nozaki, H. 2015 Two different rickettsial bacteria invading Volvox carteri. PLoS One, 10, e0116192 (article ID).
Strain(s): 397398732733866 
PubMed: 25671568
DOI: 10.1371/journal.pone.0116192

Hiraide, R., Kawai-Toyooka, H., Hamaji, T ., Matsuzaki, R., Kawafune, K., Abe, J., Sekimoto, H., Umen, J., Nozaki, H. 2013 The evolution of male–female sexual dimorphism predates the gender-based divergence of the mating locus gene MAT3/RB. Mol. Biol. Evol., 30, 1038-1040.
Keywords: gender-based divergence; gene conversion; male–female sexual dimorphism; MAT3/RB, mating type locus; volvocine algae
Strain(s): 722733866171017111859186139854018 
PubMed: 23364323
DOI: 10.1093/molbev/mst018

Ferris, P., Olson, B. J., De Hoff, P. L., Douglass, S., Casero, D., Prochnik, S., Geng, S., Rai, R., Grimwood, J., Schmutz, J., Nishii, I., Hamaji, T., Nozaki, H., Pellegrini, M., Umen,J. G. 2010 Evolution of an expanded sex-determining locus in Volvox. Science, 328, 351-354.
Strain(s): 732733 
PubMed: 20395508
DOI: 10.1126/science.1186222

Nozaki, H. 1988 Morphology, sexual reproduction and taxonomy of Volvox carteri f. kawasakiensis f. nov. (Chlorophyta) from Japan. Phycologia, 27, 209-220.
Strain(s): 580581732733 
DOI: 10.2216/i0031-8884-27-2-209.1

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