Strain Data

Strain number		NIES-121
Phylum		Heterokontophyta
Class		Raphidophyceae
Scientific name		Chattonella marina var. marina (Subrahmanyan) Y.Hara & Chihara
Synonym		Hornellia marina Subrahmanyan 1954
Former name		Chattonella marina (Subrahmanyan) Hara et Chihara
Common name
Locality (Date of collection)		Kagoshima Bay, Kagoshima, Japan (1982--)
Latitude / Longitude
Habitat (Isolation source)		Marine (Seawater)
History		< Aramaki, Takayuki
Isolator (Date of isolation)		Aramaki, Takayuki (1982--)
Identified by		Aramaki, Takayuki
State of strain		Subculture; Unialgal; Clonal; Non-axenic[2021 Sept]
Culture condition (Preculture condition)		Medium: f/2 Temperature: 20 C Light intensity: 13-16 µmol photons/m²/sec, L/D cycle: 10L:14D Duration: 1 M
Gene information		18S rRNA ( AB217627 ) , 28S rRNA ( AB217635 ) , 18S-ITS1-5.8S-ITS2-28S ( AB286919 ) , CO2, CO1 ( AB286889 ) , CO1 ( AB445423 ) , trnE, trnS ( AB570015 ) , trnN, trnI, trnL, rps12 ( AB570003 )
Cell size (min - max)
Organization		Unicellular; Flagellate
Characteristics		Red tide
Other strain no.		Other strain no. : KGO-57-1
Remarks		Fragile species to transportation stresses; Read and agree "How to order 4.1"
Movie

Reference
Demura, M., Nakayama, T., Kasai, F., Kawachi, M. 2014 Genetic structure of Japanese Chattonella marina (Raphidophyceae) populations revealed using microsatellite markers. Phycol. Res., 62, 102-108. Keywords: Chattonella marina var. antiqua; Chattonella marina var. marina; genetic structure; isolation by distance; microsatellite marker; population genetics Strain(s): 1, 2, 3, 14, 83, 84, 85, 86, 113, 114, 115, 116, 118, 121, 161, 557, 1974, 1975, 1976, 1977, 1978, 1980, 1981, 1982, 1983, 1984, 2383, 2384, 2385, 2386, 2387, 2388, 2389, 2390, 2391, 2392, 2393, 2394, 2395, 2396, 2397, 2398, 2399, 2400, 2401, 2402, 2403, 2404, 2405, 2406, 2407, 2509, 2510, 2511, 2512, 2513, 2514, 2515, 2516, 2517, 2518 DOI: 10.1111/pre.12047 Masuda, I., Kamikawa, R., Ueda, M., Oyama, K., Yoshimatsu, S., Inagaki, Y., Sako, Y. 2011 Mitochondrial genomes from two red tide forming raphidophycean algae Heterosigma akashiwo and Chattonella marina var. marina. Int. J. Syst. Evol. Microbiol., 10, 130-137. Keywords: Chattonella; Genome evolution; Heterosigma; Mitochondrial genome; Red tide Strain(s): 115, 121, 557, 558, 603 DOI: 10.1016/j.hal.2010.08.004 Sakata, T., Yoshikawa, T., Nishitarumizu, S. 2010 Purification of an algicidal substance produced by marine Pseudomonas sp. C55a-2. Mem, Fac. Fish. Kagoshima Univ., 59, 17-25. Keywords: algicidal substance; Chaetoceros; Chattonella; Heterosigma; Pseudomona Strain(s): 6, 121 Kamikawa, R., Masuda, I., Demura, M., Oyama, K., Yoshimatsu, S., Kawachi, M., Sako, Y. 2009 Mitochondrial group II introns in the Raphidophycean flagellate Chattonella spp. suggest a diatom-to-Chattonella lateral group II intron transfer. Protist, 160, 364-375. Keywords: Chattonella; cytochrome c oxidase subunit 1 (cox1); diatoms; group II intron; lateral transfer; Raphidophyceae Strain(s): 1, 2, 3, 14, 85, 86, 115, 118, 121, 161, 462, 557, 558, 603, 671, 716, 849, 1303, 1975, 1976, 2391, 2401, 2402, 2403, 2404, 3422, 3429 PubMed: 19346162 DOI: 10.1016/j.protis.2009.02.003 Kamikawa, R., Masuda, I., Oyama, K., Yoshimatsu, S., Sako, Y. 2007 Genetic variation in mitochondrial genes and intergenic spacer region in harmful algae Chattonella species. Fish. Sci., 73, 871-880. Keywords: Chattonella sp.; cytochrome c oxidase subunit 1 (cox1); cytochrome c oxidase subunit 2 (cox2); genetic diversity; harmful algae; mitochondria Strain(s): 1, 2, 3, 14, 85, 86, 115, 118, 121, 161, 557, 558, 603, 671, 849, 3422, 3428, 3429 DOI: 10.1111/j.1444-2906.2007.01408.x Kamikawa, R., Asai, J., Miyahara, T., Murata, K., Oyama, K., Yoshimatsu, S., Yoshida, T., Sako, Y. 2006 Application of a real-time PCR assay to a comprehensive method of monitoring harmful algae. Mircobes Environ., 21, 163-173. Keywords: harmful algal bloom (HAB); molecular monitoring; real-time PCR; red tide Strain(s): 7, 121, 603 DOI: 10.1264/jsme2.21.163 Hosoi-Tanabe, S., Otake, I., Sako, Y. 2006 Phylogenetic analysis of noxious red tide flagellates Chattonella antiqua, C. marina, C. ovata, and C. verruculosa (Raphidophyceae) based on the rRNA gene family. Fish. Sci., 72, 1200-1208. Keywords: Chattonella; genetic homogeneity; phylogenetic analysis; Raphidophyceae; rRNA gene family Strain(s): 1, 5, 86, 121, 558, 559, 603, 670 DOI: 10.1111/j.1444-2906.2006.01277.x Nakanishi, K., Karube, I., Hiroshi, S., Uchida, A., Ishida, Y. 1996 Detection of the red tide-causing plankton Chattonella marina using a piezoelectric immunosensor. Anal. Chim. Acta, 325, 73-80. Keywords: red tide; Chattonella marina; monoclonal antibody; piezoelectric sensors Strain(s): 121 DOI: 10.1016/0003-2670(96)00006-2 Hiroishi, S., Uchida, A., Nagasaki, K., Ishida, Y. 1989 A new method for inter- and intra-species identification of red tide algae Chattonella antiqua and Chattonella marina by means of monoclonal antibodies. In Red Tides: Biology, Environmental Science, and Toxicology, Eds. by Okaichi, T., Anderson, D. M. & Nemoto, T., Elsevier Science Pub., New York, pp. 299-300. Strain(s): 85, 86, 118, 121 Uchida, A., Nagasaki, K., Hiroishi, S., Ishida, Y. 1989 The application of monoclonal antibodies to an identification of Chattonella marina and Chattonella antiqua. Bull. Jpn. Soc. Sci. Fish., 55, 721-725. Strain(s): 3, 83, 86, 118, 121 Hiroishi, S., Uchida, A., Nagasaki, K., Ishida, Y. 1988 A new method for identification of inter- and intra-species of the red tide algae Chattonella antiqua and Chattonella marina (Raphidophyceae) by means of monoclonal antibodies. J. Phycol., 24, 442-444. Keywords: Chattonella; identification; immunofluorescence; inter-species; intra-species; method; monoclonal antibody; red tide Strain(s): 1, 2, 14, 83, 84, 85, 86, 113, 114, 115, 116, 118, 121 DOI: 10.1111/j.1529-8817.1988.tb04488.x

Reference

Demura, M., Nakayama, T., Kasai, F., Kawachi, M. 2014 Genetic structure of Japanese Chattonella marina (Raphidophyceae) populations revealed using microsatellite markers. Phycol. Res., 62, 102-108.
Keywords: Chattonella marina var. antiqua; Chattonella marina var. marina; genetic structure; isolation by distance; microsatellite marker; population genetics
Strain(s): 1, 2, 3, 14, 83, 84, 85, 86, 113, 114, 115, 116, 118, 121, 161, 557, 1974, 1975, 1976, 1977, 1978, 1980, 1981, 1982, 1983, 1984, 2383, 2384, 2385, 2386, 2387, 2388, 2389, 2390, 2391, 2392, 2393, 2394, 2395, 2396, 2397, 2398, 2399, 2400, 2401, 2402, 2403, 2404, 2405, 2406, 2407, 2509, 2510, 2511, 2512, 2513, 2514, 2515, 2516, 2517, 2518
DOI: 10.1111/pre.12047

Masuda, I., Kamikawa, R., Ueda, M., Oyama, K., Yoshimatsu, S., Inagaki, Y., Sako, Y. 2011 Mitochondrial genomes from two red tide forming raphidophycean algae Heterosigma akashiwo and Chattonella marina var. marina. Int. J. Syst. Evol. Microbiol., 10, 130-137.
Keywords: Chattonella; Genome evolution; Heterosigma; Mitochondrial genome; Red tide
Strain(s): 115, 121, 557, 558, 603
DOI: 10.1016/j.hal.2010.08.004

Sakata, T., Yoshikawa, T., Nishitarumizu, S. 2010 Purification of an algicidal substance produced by marine Pseudomonas sp. C55a-2. Mem, Fac. Fish. Kagoshima Univ., 59, 17-25.
Keywords: algicidal substance; Chaetoceros; Chattonella; Heterosigma; Pseudomona
Strain(s): 6, 121

Kamikawa, R., Masuda, I., Demura, M., Oyama, K., Yoshimatsu, S., Kawachi, M., Sako, Y. 2009 Mitochondrial group II introns in the Raphidophycean flagellate Chattonella spp. suggest a diatom-to-Chattonella lateral group II intron transfer. Protist, 160, 364-375.
Keywords: Chattonella; cytochrome c oxidase subunit 1 (cox1); diatoms; group II intron; lateral transfer; Raphidophyceae
Strain(s): 1, 2, 3, 14, 85, 86, 115, 118, 121, 161, 462, 557, 558, 603, 671, 716, 849, 1303, 1975, 1976, 2391, 2401, 2402, 2403, 2404, 3422, 3429
PubMed: 19346162
DOI: 10.1016/j.protis.2009.02.003

Kamikawa, R., Masuda, I., Oyama, K., Yoshimatsu, S., Sako, Y. 2007 Genetic variation in mitochondrial genes and intergenic spacer region in harmful algae Chattonella species. Fish. Sci., 73, 871-880.
Keywords: Chattonella sp.; cytochrome c oxidase subunit 1 (cox1); cytochrome c oxidase subunit 2 (cox2); genetic diversity; harmful algae; mitochondria
Strain(s): 1, 2, 3, 14, 85, 86, 115, 118, 121, 161, 557, 558, 603, 671, 849, 3422, 3428, 3429
DOI: 10.1111/j.1444-2906.2007.01408.x

Kamikawa, R., Asai, J., Miyahara, T., Murata, K., Oyama, K., Yoshimatsu, S., Yoshida, T., Sako, Y. 2006 Application of a real-time PCR assay to a comprehensive method of monitoring harmful algae. Mircobes Environ., 21, 163-173.
Keywords: harmful algal bloom (HAB); molecular monitoring; real-time PCR; red tide
Strain(s): 7, 121, 603
DOI: 10.1264/jsme2.21.163

Hosoi-Tanabe, S., Otake, I., Sako, Y. 2006 Phylogenetic analysis of noxious red tide flagellates Chattonella antiqua, C. marina, C. ovata, and C. verruculosa (Raphidophyceae) based on the rRNA gene family. Fish. Sci., 72, 1200-1208.
Keywords: Chattonella; genetic homogeneity; phylogenetic analysis; Raphidophyceae; rRNA gene family
Strain(s): 1, 5, 86, 121, 558, 559, 603, 670
DOI: 10.1111/j.1444-2906.2006.01277.x

Nakanishi, K., Karube, I., Hiroshi, S., Uchida, A., Ishida, Y. 1996 Detection of the red tide-causing plankton Chattonella marina using a piezoelectric immunosensor. Anal. Chim. Acta, 325, 73-80.
Keywords: red tide; Chattonella marina; monoclonal antibody; piezoelectric sensors
Strain(s): 121
DOI: 10.1016/0003-2670(96)00006-2

Hiroishi, S., Uchida, A., Nagasaki, K., Ishida, Y. 1989 A new method for inter- and intra-species identification of red tide algae Chattonella antiqua and Chattonella marina by means of monoclonal antibodies. In Red Tides: Biology, Environmental Science, and Toxicology, Eds. by Okaichi, T., Anderson, D. M. & Nemoto, T., Elsevier Science Pub., New York, pp. 299-300.
Strain(s): 85, 86, 118, 121

Uchida, A., Nagasaki, K., Hiroishi, S., Ishida, Y. 1989 The application of monoclonal antibodies to an identification of Chattonella marina and Chattonella antiqua. Bull. Jpn. Soc. Sci. Fish., 55, 721-725.
Strain(s): 3, 83, 86, 118, 121

Hiroishi, S., Uchida, A., Nagasaki, K., Ishida, Y. 1988 A new method for identification of inter- and intra-species of the red tide algae Chattonella antiqua and Chattonella marina (Raphidophyceae) by means of monoclonal antibodies. J. Phycol., 24, 442-444.
Keywords: Chattonella; identification; immunofluorescence; inter-species; intra-species; method; monoclonal antibody; red tide
Strain(s): 1, 2, 14, 83, 84, 85, 86, 113, 114, 115, 116, 118, 121
DOI: 10.1111/j.1529-8817.1988.tb04488.x

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