Strain Data

Strain number		NIES-118
Phylum		Heterokontophyta
Class		Raphidophyceae
Scientific name		Chattonella marina var. marina (Subrahmanyan) Y.Hara & Chihara
Synonym		Hornellia marina Subrahmanyan 1954
Former name		Chattonella marina (Subrahmanyan) Hara et Chihara
Common name
Locality (Date of collection)		Harima-Nada, Seto Inland Sea Japan (1983-07-29)
Latitude / Longitude
Habitat (Isolation source)		Marine (Seawater)
History		< KAGAWA
Isolator (Date of isolation)		Yoshimatsu, Sadaaki
Identified by		Yoshimatsu, Sadaaki
State of strain		Subculture; Unialgal; Clonal; Axenic[2018 Feb]
Culture condition (Preculture condition)		Medium: f/2 Temperature: 20 C Light intensity: 13-16 µmol photons/m²/sec, L/D cycle: 10L:14D Duration: 1 M
Gene information		18S-ITS1-5.8S-ITS2-28S ( AB286913 ) , ITS1-5.8S-ITS2 ( AB334331 ) , CO1 ( AB334468 ) , rbcL ( AB334395 ) , CO2, CO1 ( AB286902 ) , CO1 ( AB445422 )
Cell size (min - max)
Organization		Unicellular; Flagellate
Characteristics		Red tide
Other strain no.		Other strain no. : KGW-75-2
Remarks		Fragile species to transportation stresses; Read and agree "How to order 4.1"; Axenic
Movie

Reference
Demura, M., Nakayama, T., Kasai, F., Kawachi, M. 2014 Genetic structure of Japanese Chattonella marina (Raphidophyceae) populations revealed using microsatellite markers. Phycol. Res., 62, 102-108. Keywords: Chattonella marina var. antiqua; Chattonella marina var. marina; genetic structure; isolation by distance; microsatellite marker; population genetics Strain(s): 1, 2, 3, 14, 83, 84, 85, 86, 113, 114, 115, 116, 118, 121, 161, 557, 1974, 1975, 1976, 1977, 1978, 1980, 1981, 1982, 1983, 1984, 2383, 2384, 2385, 2386, 2387, 2388, 2389, 2390, 2391, 2392, 2393, 2394, 2395, 2396, 2397, 2398, 2399, 2400, 2401, 2402, 2403, 2404, 2405, 2406, 2407, 2509, 2510, 2511, 2512, 2513, 2514, 2515, 2516, 2517, 2518 DOI: 10.1111/pre.12047 Dorantes-Aranda, J. J., Nichols, P. D., Waite, T. D., Hallegraeff, G. M. 2013 Strain variability in fatty acid composition of Chattonella marina (Raphidophyceae) and its relation to differing ichthyotoxicity toward rainbow trout gill cells. J. Phycol., 2, 427-438. Keywords: Aldehydes; Chattonella marina; fatty acids; harmful phytoplankton; ichthyotoxicity; rainbow trout gill cell line; RT gill-W1 Strain(s): 118 PubMed: 27008528 DOI: 10.1111/jpy.12053 Kamikawa, R., Masuda, I., Demura, M., Oyama, K., Yoshimatsu, S., Kawachi, M., Sako, Y. 2009 Mitochondrial group II introns in the Raphidophycean flagellate Chattonella spp. suggest a diatom-to-Chattonella lateral group II intron transfer. Protist, 160, 364-375. Keywords: Chattonella; cytochrome c oxidase subunit 1 (cox1); diatoms; group II intron; lateral transfer; Raphidophyceae Strain(s): 1, 2, 3, 14, 85, 86, 115, 118, 121, 161, 462, 557, 558, 603, 671, 716, 849, 1303, 1975, 1976, 2391, 2401, 2402, 2403, 2404, 3422, 3429 PubMed: 19346162 DOI: 10.1016/j.protis.2009.02.003 Demura, M., Noël, M.-H., Kasai, F., Watanabe, M. M., Kawachi, M. 2009 Taxonomic revision of Chattonella antiqua, C. marina and C. ovata (Raphidophyceae) based on their morphological characteristics and genetic diversity. Phycologia, 48, 518-535. Keywords: Chattonella antiqua; Chattonella marina; Chattonella ovata; COI; COX1; Genetic diversity; ITS; Microsatellite; Morphology; Phylogeny; Population; Raphidophyceae; rbcL; Speciation; Taxonomy Strain(s): 1, 2, 3, 5, 6, 9, 10, 14, 83, 84, 85, 113, 114, 116, 118, 146, 161, 293, 557, 561, 603, 671, 716, 849, 1974, 1975, 1976, 1977, 1978, 1980, 1981, 1982, 1983, 1984, 1986, 2383, 2384, 2385, 2386, 2387, 2388, 2389, 2391, 2392, 2393, 2394, 2395, 2396, 2397, 2398, 2399, 2400, 2401, 2402, 2403, 2404, 2405, 2406, 2407 DOI: 10.2216/08-98.1 Hamana, K. 2008 Cellular polyamines of phototrophs and beterotrophs belonging to the lower eukaryotic phyla Cercozoa, Euglenozoa, Heterokonta and Metamonada. J. Gen. Appl. Microbiol., 54, 135-140. Keywords: cyanobacteria; homospermidine; polyamine; spermine; thermospermine Strain(s): 9, 15, 71, 86, 118, 253, 323, 330, 333, 548, 603, 622, 1002, 1003, 1011, 1044, 1303, 1380, 1440, 1443, 1444 PubMed: 18497488 DOI: 10.2323/jgam.54.135 Kamikawa, R., Masuda, I., Oyama, K., Yoshimatsu, S., Sako, Y. 2007 Genetic variation in mitochondrial genes and intergenic spacer region in harmful algae Chattonella species. Fish. Sci., 73, 871-880. Keywords: Chattonella sp.; cytochrome c oxidase subunit 1 (cox1); cytochrome c oxidase subunit 2 (cox2); genetic diversity; harmful algae; mitochondria Strain(s): 1, 2, 3, 14, 85, 86, 115, 118, 121, 161, 557, 558, 603, 671, 849, 3422, 3428, 3429 DOI: 10.1111/j.1444-2906.2007.01408.x Marshall, J. A. & Newman, S. 2002 Differences in photoprotective pigment production between Japanese and Australian strains of Chattonella marina (Raphidophyceae). J. Exp. Mar. Biol. Ecol., 272, 13-27. Keywords: ultraviolet radiation; microalgae; raphidophytes; Chattonella; mycosporine amino acids; pigments; xanthophyll cycle; stratospheric ozone depletion Strain(s): 1, 118 DOI: 10.1016/S0022-0981(02)00034-5 Marshall, J. A., Hovenden, M., Oda, T., Hallegraeff, G. M. 2002 Photosynthesis does influence superoxide production in the ichthyotoxic alga Chattonella marina (Raphidophyceae). J. Plankton Res., 24, 1231-1236. Strain(s): 118 DOI: 10.1093/plankt/24.11.1231 Marshall, J. A., Nichols, P. D., Hallegraeff, G. M. 2002 Chemotaxonomic survey of sterols and fatty acids in six marine raphidophyte algae. J. Appl. Phycol., 14, 255-265. Keywords: Chattonella antiqua; Chattonella marina; Chattonella subsalsa; chemotaxonomy; fatty acids; Fibrocapsa japonica; Heterosigma akashiwo; Olisthodiscus luteus; phycotoxins; raphydophytes; sterols Strain(s): 15, 118 Marshall, J. A., Munday, B., Yoshizawa, Y., Hallegraeff, G. M. 2001 Effect of irradiance of superoxide production by Chattonella marina (Raphidophyceae) from south Australia and Japan. In Harmful Algal Blooms 2000, Eds. by Hallegraeff et al., UNESCO 2001., pp. 316-319. Strain(s): 118 Marshall, J. A. & Hallegraeff, G. M. 1999 Comparative ecophysiology of the harmful alga Chattonella marina (Raphidophyceae) from South Australian and Japanese waters. J. Plankton Res., 21, 1809-1822. Strain(s): 118 DOI: 10.1093/plankt/21.10.1809 Sako, Y., Adachi, M., Ishida, Y. 1995 Specific monoclonal antibodies and DNA probes for the identification of the toxic dinoflagellate genus Alexandrium. In Harmful Marine Algal Blooms, Eds. by Lassus, P., et al., Lavoisier, Intercept Ltd., pp. 77-82. Strain(s): 5, 118 Hara, Y., Doi, K., Chihara, M. 1994 Four new species of Chattonella (Raphidophyceae, Chromophyta) from Japan. Jpn. J. Phycol. (Sôrui), 42, 407-420. Keywords: Chattonella; C. globosa; C. minima; C. ovata; C. verruculosa; Raphidophyceae; red tide organisms; taxonomy Strain(s): 118, 161, 603, 670, 849 Hiroishi, S., Uchida, A., Nagasaki, K., Ishida, Y. 1989 A new method for inter- and intra-species identification of red tide algae Chattonella antiqua and Chattonella marina by means of monoclonal antibodies. In Red Tides: Biology, Environmental Science, and Toxicology, Eds. by Okaichi, T., Anderson, D. M. & Nemoto, T., Elsevier Science Pub., New York, pp. 299-300. Strain(s): 85, 86, 118, 121 Uchida, A., Nagasaki, K., Hiroishi, S., Ishida, Y. 1989 The application of monoclonal antibodies to an identification of Chattonella marina and Chattonella antiqua. Bull. Jpn. Soc. Sci. Fish., 55, 721-725. Strain(s): 3, 83, 86, 118, 121 Hiroishi, S., Uchida, A., Nagasaki, K., Ishida, Y. 1988 A new method for identification of inter- and intra-species of the red tide algae Chattonella antiqua and Chattonella marina (Raphidophyceae) by means of monoclonal antibodies. J. Phycol., 24, 442-444. Keywords: Chattonella; identification; immunofluorescence; inter-species; intra-species; method; monoclonal antibody; red tide Strain(s): 1, 2, 14, 83, 84, 85, 86, 113, 114, 115, 116, 118, 121 DOI: 10.1111/j.1529-8817.1988.tb04488.x

Reference

Demura, M., Nakayama, T., Kasai, F., Kawachi, M. 2014 Genetic structure of Japanese Chattonella marina (Raphidophyceae) populations revealed using microsatellite markers. Phycol. Res., 62, 102-108.
Keywords: Chattonella marina var. antiqua; Chattonella marina var. marina; genetic structure; isolation by distance; microsatellite marker; population genetics
Strain(s): 1, 2, 3, 14, 83, 84, 85, 86, 113, 114, 115, 116, 118, 121, 161, 557, 1974, 1975, 1976, 1977, 1978, 1980, 1981, 1982, 1983, 1984, 2383, 2384, 2385, 2386, 2387, 2388, 2389, 2390, 2391, 2392, 2393, 2394, 2395, 2396, 2397, 2398, 2399, 2400, 2401, 2402, 2403, 2404, 2405, 2406, 2407, 2509, 2510, 2511, 2512, 2513, 2514, 2515, 2516, 2517, 2518
DOI: 10.1111/pre.12047

Dorantes-Aranda, J. J., Nichols, P. D., Waite, T. D., Hallegraeff, G. M. 2013 Strain variability in fatty acid composition of Chattonella marina (Raphidophyceae) and its relation to differing ichthyotoxicity toward rainbow trout gill cells. J. Phycol., 2, 427-438.
Keywords: Aldehydes; Chattonella marina; fatty acids; harmful phytoplankton; ichthyotoxicity; rainbow trout gill cell line; RT gill-W1
Strain(s): 118
PubMed: 27008528
DOI: 10.1111/jpy.12053

Kamikawa, R., Masuda, I., Demura, M., Oyama, K., Yoshimatsu, S., Kawachi, M., Sako, Y. 2009 Mitochondrial group II introns in the Raphidophycean flagellate Chattonella spp. suggest a diatom-to-Chattonella lateral group II intron transfer. Protist, 160, 364-375.
Keywords: Chattonella; cytochrome c oxidase subunit 1 (cox1); diatoms; group II intron; lateral transfer; Raphidophyceae
Strain(s): 1, 2, 3, 14, 85, 86, 115, 118, 121, 161, 462, 557, 558, 603, 671, 716, 849, 1303, 1975, 1976, 2391, 2401, 2402, 2403, 2404, 3422, 3429
PubMed: 19346162
DOI: 10.1016/j.protis.2009.02.003

Demura, M., Noël, M.-H., Kasai, F., Watanabe, M. M., Kawachi, M. 2009 Taxonomic revision of Chattonella antiqua, C. marina and C. ovata (Raphidophyceae) based on their morphological characteristics and genetic diversity. Phycologia, 48, 518-535.
Keywords: Chattonella antiqua; Chattonella marina; Chattonella ovata; COI; COX1; Genetic diversity; ITS; Microsatellite; Morphology; Phylogeny; Population; Raphidophyceae; rbcL; Speciation; Taxonomy
Strain(s): 1, 2, 3, 5, 6, 9, 10, 14, 83, 84, 85, 113, 114, 116, 118, 146, 161, 293, 557, 561, 603, 671, 716, 849, 1974, 1975, 1976, 1977, 1978, 1980, 1981, 1982, 1983, 1984, 1986, 2383, 2384, 2385, 2386, 2387, 2388, 2389, 2391, 2392, 2393, 2394, 2395, 2396, 2397, 2398, 2399, 2400, 2401, 2402, 2403, 2404, 2405, 2406, 2407
DOI: 10.2216/08-98.1

Hamana, K. 2008 Cellular polyamines of phototrophs and beterotrophs belonging to the lower eukaryotic phyla Cercozoa, Euglenozoa, Heterokonta and Metamonada. J. Gen. Appl. Microbiol., 54, 135-140.
Keywords: cyanobacteria; homospermidine; polyamine; spermine; thermospermine
Strain(s): 9, 15, 71, 86, 118, 253, 323, 330, 333, 548, 603, 622, 1002, 1003, 1011, 1044, 1303, 1380, 1440, 1443, 1444
PubMed: 18497488
DOI: 10.2323/jgam.54.135

Kamikawa, R., Masuda, I., Oyama, K., Yoshimatsu, S., Sako, Y. 2007 Genetic variation in mitochondrial genes and intergenic spacer region in harmful algae Chattonella species. Fish. Sci., 73, 871-880.
Keywords: Chattonella sp.; cytochrome c oxidase subunit 1 (cox1); cytochrome c oxidase subunit 2 (cox2); genetic diversity; harmful algae; mitochondria
Strain(s): 1, 2, 3, 14, 85, 86, 115, 118, 121, 161, 557, 558, 603, 671, 849, 3422, 3428, 3429
DOI: 10.1111/j.1444-2906.2007.01408.x

Marshall, J. A. & Newman, S. 2002 Differences in photoprotective pigment production between Japanese and Australian strains of Chattonella marina (Raphidophyceae). J. Exp. Mar. Biol. Ecol., 272, 13-27.
Keywords: ultraviolet radiation; microalgae; raphidophytes; Chattonella; mycosporine amino acids; pigments; xanthophyll cycle; stratospheric ozone depletion
Strain(s): 1, 118
DOI: 10.1016/S0022-0981(02)00034-5

Marshall, J. A., Hovenden, M., Oda, T., Hallegraeff, G. M. 2002 Photosynthesis does influence superoxide production in the ichthyotoxic alga Chattonella marina (Raphidophyceae). J. Plankton Res., 24, 1231-1236.
Strain(s): 118
DOI: 10.1093/plankt/24.11.1231

Marshall, J. A., Nichols, P. D., Hallegraeff, G. M. 2002 Chemotaxonomic survey of sterols and fatty acids in six marine raphidophyte algae. J. Appl. Phycol., 14, 255-265.
Keywords: Chattonella antiqua; Chattonella marina; Chattonella subsalsa; chemotaxonomy; fatty acids; Fibrocapsa japonica; Heterosigma akashiwo; Olisthodiscus luteus; phycotoxins; raphydophytes; sterols
Strain(s): 15, 118

Marshall, J. A., Munday, B., Yoshizawa, Y., Hallegraeff, G. M. 2001 Effect of irradiance of superoxide production by Chattonella marina (Raphidophyceae) from south Australia and Japan. In Harmful Algal Blooms 2000, Eds. by Hallegraeff et al., UNESCO 2001., pp. 316-319.
Strain(s): 118

Marshall, J. A. & Hallegraeff, G. M. 1999 Comparative ecophysiology of the harmful alga Chattonella marina (Raphidophyceae) from South Australian and Japanese waters. J. Plankton Res., 21, 1809-1822.
Strain(s): 118
DOI: 10.1093/plankt/21.10.1809

Sako, Y., Adachi, M., Ishida, Y. 1995 Specific monoclonal antibodies and DNA probes for the identification of the toxic dinoflagellate genus Alexandrium. In Harmful Marine Algal Blooms, Eds. by Lassus, P., et al., Lavoisier, Intercept Ltd., pp. 77-82.
Strain(s): 5, 118

Hara, Y., Doi, K., Chihara, M. 1994 Four new species of Chattonella (Raphidophyceae, Chromophyta) from Japan. Jpn. J. Phycol. (Sôrui), 42, 407-420.
Keywords: Chattonella; C. globosa; C. minima; C. ovata; C. verruculosa; Raphidophyceae; red tide organisms; taxonomy
Strain(s): 118, 161, 603, 670, 849

Hiroishi, S., Uchida, A., Nagasaki, K., Ishida, Y. 1989 A new method for inter- and intra-species identification of red tide algae Chattonella antiqua and Chattonella marina by means of monoclonal antibodies. In Red Tides: Biology, Environmental Science, and Toxicology, Eds. by Okaichi, T., Anderson, D. M. & Nemoto, T., Elsevier Science Pub., New York, pp. 299-300.
Strain(s): 85, 86, 118, 121

Uchida, A., Nagasaki, K., Hiroishi, S., Ishida, Y. 1989 The application of monoclonal antibodies to an identification of Chattonella marina and Chattonella antiqua. Bull. Jpn. Soc. Sci. Fish., 55, 721-725.
Strain(s): 3, 83, 86, 118, 121

Hiroishi, S., Uchida, A., Nagasaki, K., Ishida, Y. 1988 A new method for identification of inter- and intra-species of the red tide algae Chattonella antiqua and Chattonella marina (Raphidophyceae) by means of monoclonal antibodies. J. Phycol., 24, 442-444.
Keywords: Chattonella; identification; immunofluorescence; inter-species; intra-species; method; monoclonal antibody; red tide
Strain(s): 1, 2, 14, 83, 84, 85, 86, 113, 114, 115, 116, 118, 121
DOI: 10.1111/j.1529-8817.1988.tb04488.x

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