Strain number NIES-611  
Phylum Cyanophyta  
Class Cyanophyceae  
Scientific name Pseudanabaena sp.  
Synonym  
Former name Phormidium tenue (C.Agardh ex Gomont) Anagnostidis et Komárek  
Common name Blue-green alga ; Cyanobacteria  
Locality (Date of collection) Lake Biwa Shiga Japan  
Latitude / Longitude 35.2852741059 / 136.08878954 
Habitat (Isolation source) Freshwater (Lake water)  
History  
Isolator (Date of isolation)  
Identified by Watanabe, Makoto M.; Homma, Takamitsu (Reidentify)  
State of strain Cryopreservation; Unialgal; Clonal; Non-axenic  
Culture condition
(Preculture condition)
Medium:  CT  
Temperature:  25 C
Light intensity:  100-120 µmol photons/m2/sec, L/D cycle:  10L:14D
Duration:  1 M  
Gene information 16S rRNA ( AB042842 )  
Cell size (min - max) 3 - μm  
Organization Filamentous 
Characteristics  
Other strain no. Other strain no. : Bpt  
Remarks Cryopreserved 
Movie  
Reference
Asukabe, H., Akahori, S., Ueno, E., Nakayama, T., Yamashita, R., Arii, S., Harada, K., Imanishi, S. Y. 2020 Cyanobacterial classification with the toxicity using MALDI Biotyper. J. Am. Soc. Mass Spectrom., 31, 1572-1578.
Keywords: cyanobacteria; MALDI Biotyper; microcystins; profiling
Strain(s): 27394273818789100101102103104105107111112207208230266298416433478510512515592593604611808809835843846847905928931932945981989102510431058106110641068106910711076108510991101110511131115112611331134113511441157121516421643165216681672167316771678169820872131241226653708373337343736375537563757 
PubMed: 32501712
DOI: 10.1021/jasms.0c00148

Imanishi, S. Y., Nakayama, T., Asukabe, H., Harada, K. 2017 Application of MALDI Biotyper to cyanobacterial profiling. Rapid Commun. Mass Spectrom., 31, 325-332.
Strain(s): 738910210310711229861180880983584398910251043105810711099164216521672 
PubMed: 27862451
DOI: 10.1002/rcm.7793

Harada, K-I., Ozaki, K., Tsuzuki, S., Kato, H., Hasegawa, M., Kuroda, E. K., Arii, S., Tsuji, K. 2009 Blue color formation of cyanobacteria with b-cyclocitral. J. Chem. Ecol., 35, 1295-1301.
Keywords: b-cyclocitral; cyanobacteria; lysis; blue color formation; acid stress
Strain(s): 73102103112298611808 
PubMed: 19936836
DOI: 10.1007/s10886-009-9706-5

Tomioka, N., Nagai, T., Kawasaki, T., Imai, A., Kohata, K. 2008 Quantification of Microcystis in a eutrophic lake by simple DNA extraction and SYBR green real-time PCR. Microbes Environ., 23, 306-312.
Keywords: Microcystis; quantification; SYBR Green I; DNA extraction; eutrophic lake
Strain(s): 9899204604611843946961 
DOI: 10.1264/jsme2.ME08515

Oikawa, E. Ishibashi, Y., 2004 Species specificity of musty odor producing Phormidium tenue in Lake Kamafusa. Wat. Sci. Tech., 49, 41-46.
Keywords: 16S-rDNA sequence; 2-methylisoborneol; musty odor; Phormidium tenue phylogenetic tree
Strain(s): 213033417376512611 
PubMed: 15237605
DOI: 10.2166/wst.2004.0529

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