Strain number NIES-2342  
Phylum Chlorophyta  
Class Trebouxiophyceae  
Scientific name Choricystis krienitzii Pröschold & Darienko  
Former name Formerly identified as Auxenochlorella sp., re-identified at NIES by DNA analysis  
Common name Green alga  
Locality (Date of collection) Lake Otadomarinuma, Hokkaido, Japan (1991-05-29)  
Latitude / Longitude 45.120023 / 141.285551 
Habitat (Isolation source) Freshwater (Lake water)  
History < Takamura, Noriko  
Isolator (Date of isolation) Takamura, Noriko (1991-07-**)  
Identified by Re-identified by NIES  
State of strain Cryopreservation; Unialgal  
Culture condition
(Preculture condition)
Medium:  CB  
Temperature:  15 C
Light intensity:  15-22 µmol photons/m2/sec, L/D cycle:  10L:14D
Duration:  2 M  
Gene information 18S rRNA ( AB488587 )  
Cell size (min - max)  
Organization Unicellular 
Other strain no. Other strain no. : OTD 1  
Remarks Cryopreserved 
Pröschold, T., Darienko, T. 2020 Choricystis and Lewiniosphaera gen. nov. (Trebouxiophyceae Chlorophyta), two different green algal endosymbionts in freshwater sponges. Symbiosis, 82, 175-188.
Keywords: Choricystis; Lewiniosphaera; Freshwater sponges; Endosymbiosis
Strain(s): 21672171234240334292 
PubMed: 33328698
DOI: 10.1007/s13199-020-00711-x

Zahradníková, M. 2017 Taxonomy and phylogeny of the family Fuscideaceae (Umbilicariales, Ascomycota) with special emphasis on Fuscidea. PhD thesis, University of Bergen, Norway, 196 pp.
Strain(s): 640215023422352 

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