Strain number NIES-2339  
Phylum Chlorophyta  
Class Chlorophyceae  
Scientific name Mychonastes frigidus E.Patova, I.Novakovskaya, Martinenko, E.Gusev & M.Kulikovskiy  
Synonym  
Former name Mychonastes sp.  
Common name Green alga  
Locality (Date of collection) Lake Sagami, Kanagawa, Japan (1991-04-11)  
Latitude / Longitude 35.6127439121 / 139.18283003 
Habitat (Isolation source) Freshwater (Lake water)  
History < Takamura, Noriko  
Isolator (Date of isolation) Takamura, Noriko (1991-05-**)  
Identified by Nozaki, Hisayoshi (Reidentify)  
State of strain Cryopreservation; Unialgal; Clonal; Non-axenic[2016 May]  
Culture condition
(Preculture condition)
Medium:  CB  
Temperature:  15 C
Light intensity:  19 µmol photons/m2/sec, L/D cycle:  10L:14D
Duration:  4 M  
Gene information 18S rRNA ( AB488566 ) , 18S-ITS1-5.8S-ITS2-28S ( LC853069 ) , rbcL ( LC853336 ) , atpB ( LC853351 ) , psaB ( LC853366 ) , psbC ( LC853381 )  
Cell size (min - max) 2 - 3 μm  
Organization Unicellular 
Characteristics  
Other strain no. Other strain no. : SA 2  
Remarks Cryopreserved 
Movie  
Reference
Nozaki, H., Matsuzaki, R., Mori, F., Sato, M., Yamaguchi, H., Higashiyama, T., Kawachi, M., Tanabe, Y. 2025 Description of four new species of Mychonastes (Chlorophyceae) from Japanese freshwater habitats based on molecular and morphological data. Phycol. Res., 73, 35-48.
Keywords: freshwater green alga; molecular phylogeny; morphology; Mychonastes; Mychonastes ariiae sp. nov.; Mychonastes gigas sp. nov.; Mychonastes kasaiae sp. nov.; Mychonastes takamurae sp. nov.; species; taxonomy
Strain(s): 23342336233923402341289729003742374345464547 
DOI: 10.1111/pre.12573

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