Strain Data

A big image opens when the thumbnail is clicked.(nies-2330.jpg)

Strain number		NIES-2330
Phylum		Chlorophyta
Class		Trebouxiophyceae
Scientific name		Didymogenes soliella Hoshina & Fujiwara
Synonym
Former name		Chlorella sp.
Common name		Green alga
Locality (Date of collection)		Lake Kasumigaura, Miho, Ibaraki, Japan (1991-04-22)
Latitude / Longitude		36.0428477398 / 140.373233439
Habitat (Isolation source)		Freshwater (Pond sediment)
History		< Watanabe, Yoshitomo
Isolator (Date of isolation)		Watanabe, Yoshitomo (1991-06-**)
Identified by		Hoshina, Ryo (Reidentify)
State of strain		Cryopreservation; Unialgal; Clonal; Axenic[2018 Mar]
Culture condition (Preculture condition)		Medium: C (agar) Temperature: 20 (25) C Light intensity: 4-10 (14-16) µmol photons/m²/sec, L/D cycle: 10L:14D Duration: 3 M (14 D)
Gene information		18S-ITS1-5.8S-ITS2-28S ( AB731605 )
Cell size (min - max)		2 - 7 μm
Organization		Unicellular
Characteristics		Authentic strain (Hoshina et al. 2013)
Other strain no.		Other strain no. : HA-1
Remarks		Cryopreserved; Axenic
Movie

Reference
Krivina, E., Portnov, A., Temraleeva, A. 2024 A description of Aliichlorella ignota gen. et sp. nov. and a comparison of the efficiency of species delimitation methods in the Chlorella-clade (Trebouxiophyceae, Chlorophyta). Phycol. Res., 72, 180-190. Strain(s): 455, 2167, 2171, 2173, 2330, 4032, 4033, 4034 DOI: 10.1111/pre.12551 Hoshina, R. & Fujiwara, Y. 2013 Molecular characterization of Chlorella cultures of the National Institute for Environmental Studies culture collection with description of Micractinium inermum sp. nov., Didymogenes sphaerica sp. nov., Didymogenes soliella sp. nov., (Chlorellaceae, Trebouxiophyceae). Phycol. Res., 61, 124-132. Keywords: Chlorella; Didymogenes; group 1 intron; internal transcribed spacer 2; Micractinium; species concept Strain(s): 2167, 2171, 2173, 2330 DOI: 10.1111/pre.12010

Reference

Krivina, E., Portnov, A., Temraleeva, A. 2024 A description of Aliichlorella ignota gen. et sp. nov. and a comparison of the efficiency of species delimitation methods in the Chlorella-clade (Trebouxiophyceae, Chlorophyta). Phycol. Res., 72, 180-190.
Strain(s): 455, 2167, 2171, 2173, 2330, 4032, 4033, 4034
DOI: 10.1111/pre.12551

Hoshina, R. & Fujiwara, Y. 2013 Molecular characterization of Chlorella cultures of the National Institute for Environmental Studies culture collection with description of Micractinium inermum sp. nov., Didymogenes sphaerica sp. nov., Didymogenes soliella sp. nov., (Chlorellaceae, Trebouxiophyceae). Phycol. Res., 61, 124-132.
Keywords: Chlorella; Didymogenes; group 1 intron; internal transcribed spacer 2; Micractinium; species concept
Strain(s): 2167, 2171, 2173, 2330
DOI: 10.1111/pre.12010

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