Strain number NIES-2330  
Phylum Chlorophyta  
Class Trebouxiophyceae  
Scientific name Didymogenes soliella Hoshina & Fujiwara  
Former name Chlorella sp.  
Common name Green alga  
Locality (Date of collection) Lake Kasumigaura, Miho, Ibaraki, Japan (1991-04-22)  
Latitude / Longitude 36.0428477398 / 140.373233439 
Habitat (Isolation source) Freshwater (Pond sediment)  
History < Watanabe, Yoshitomo  
Isolator (Date of isolation) Watanabe, Yoshitomo (1991-06-**)  
Identified by Hoshina, Ryo (Reidentify)  
State of strain Cryopreservation; Unialgal; Clonal; Axenic[2018 Mar]  
Culture condition
(Preculture condition)
Medium:  C (agar)  
Temperature:  20 (25) C
Light intensity:  4-10 (14-16) µmol photons/m2/sec, L/D cycle:  10L:14D
Duration:  3 M (14 D)  
Gene information 18S-ITS1-5.8S-ITS2-28S ( AB731605 )  
Cell size (min - max) 2 - 7 μm  
Organization Unicellular 
Characteristics Authentic strain (Hoshina et al. 2013)  
Other strain no. Other strain no. : HA-1  
Remarks Cryopreserved; Axenic 
Hoshina, R., Fujiwara, Y. 2013 Molecular characterization of Chlorella cultures of the National Institute for Environmental Studies culture collection with description of Micractinium inermum sp. nov., Didymogenes sphaerica sp. nov., Didymogenes soliella sp. nov., (Chlorellaceae, Trebouxiophyceae). Phycol. Res., 61, 124-132.
Keywords: Chlorella; Didymogenes; group 1 intron; internal transcribed spacer 2; Micractinium; species concept
Strain(s): 2167217121732330 
DOI: 10.1111/pre.12010

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