Strain number NIES-2320  
Phylum Chlorophyta  
Class Chlorophyceae  
Scientific name Rhysamphichloris similis (Korshikov) Nakada  
Synonym Chlamydomonas similis Korshikov var. similis  
Former name Chlamydomonas sp.  
Common name Green alga  
Locality (Date of collection) Wakamorishimo-ike Pond, Tsukuba, Ibaraki, Japan (1985-11-05)  
Latitude / Longitude 36.145929 / 140.083312 
Habitat (Isolation source) Freshwater (Pond water)  
History < Suda, Shoichiro  
Isolator (Date of isolation) Suda, Shoichiro (1985-11-06)  
Identified by Nakada, Takashi (Reidentify)  
State of strain Subculture; Unialgal; Clonal; Axenic[2017 Dec]  
Culture condition
(Preculture condition)
Medium:  AF-6  
Temperature:  20 C
Light intensity:  3 µmol photons/m2/sec, L/D cycle:  10L:14D
Duration:  70 D  
Gene information 18S rRNA ( LC057292 AB701531 ) , atpB ( LC133505 ) , psaB ( LC133509 ) , rbcL ( LC133513 )  
Cell size (min - max)  
Organization Unicellular; Flagellate 
Characteristics Authentic strain, Epitype (Nakada et al. 2016)  
Other strain no. Other strain no. : w-14-5  
Remarks Axenic 
Movie  
Reference
Nikulin, V. Y., Nikulin, A. Y., Gontcharov, A. A., Bagmet, V. B., Abdullin, S. R. 2023 Oogamochlamys kurilensis sp. nov. (Chlorophyta, Volvocales) from the soils of Iturup Island (Sakhalin Region, Russia). Plants-Basel, 12, 3350 (article ID).
Keywords: biflagellate green alga; Oogamochlamys; new species; SSU rDNA; ITS rDNA secondary structure; morphological characteristics; life cycle; temperate monsoon climate zone
Strain(s): 171617202209221022332240229423172318232035723760 
PubMed: 37836090
DOI: 10.3390/plants12193350

Nakada, T. & Tomita, M. 2017 Morphology and phylogeny of a new wall‐less freshwater volvocalean flagellate, Hapalochloris nozakii gen. et sp. nov. (Volvocales, Chlorophyceae). J. Phycol., 53, 108-117.
Keywords: cell wall; contractile vacuoles; Hapalochloris nozakii gen. et sp. nov.; mitochondria; molecular phylogeny; morphology; new species; taxonomy; Volvocale
Strain(s): 2254232037053760376137623763 
PubMed: 27767210
DOI: 10.1111/jpy.12484

Nakada, T., Tomita, M., Wu, J.-T., Nozaki, H. 2016 Taxonomic revision of Chlamydomonas subg. Amphichloris (Volvocales, Chlorophyceae), with resurrection of the genus Dangeardinia and Descriptions of Ixipapillifera gen. nov. and Rhysamphichloris gen. nov. J. Phycol., 52, 283-304.
Keywords: Chlamydomonas subg.; Amphichloris; Dangeardinia; Ixipapillifera; mitochondria; molecular phylogeny; morphology; new species; Rhysamphichloris; taxonomy; Volvocales
Strain(s): 2320232123222575370237033704370537063707 
PubMed: 27037593
DOI: 10.1111/jpy.12397

Yumoto, K., Kasai, F., Kawachi, M. 2013 Taxonomic re-examination of Chlamydomonas strains maintained in the NIES-Collection. Microbiol. Cult. Coll., 29, 1-12.
Strain(s): 158437438440884968102110221048173318481849220122022203220422062207220822092210221122122213221422152216221822192220222222232224222522282229223022312233223422352236223722382239224022412242224322462247224823142315231623172318231923202321232223232324246224632499257525762577257825793284 

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