Strain number NIES-485  
Phylum Chlorophyta  
Class Nephroselmidophyceae  
Scientific name Nephroselmis olivacea F.Stein  
Former name  
Common name  
Locality (Date of collection) Tsuchiura, Ibaraki, Japan (1986-02-**)  
Latitude / Longitude 36.081096 / 140.16337 
Habitat (Isolation source) Freshwater (Pond sediment)  
History < Suda, Shoichiro  
Isolator (Date of isolation) Suda, Shoichiro (1986-05-09)  
Identified by Suda, Shoichiro  
State of strain Subculture; Unialgal; Clonal; Axenic[2017 Dec]  
Culture condition
(Preculture condition)
Medium:  AF-6  
Temperature:  20 C
Light intensity:  10-15 µmol photons/m2/sec, L/D cycle:  10L:14D
Duration:  2 M  
Gene information  
Cell size (min - max)  
Organization Unicellular; Flagellate 
Characteristics Heterothallic ; Mating type (−)  
Other strain no. Other strain no. : S-N-3-4  
Remarks Axenic 
Nowack, E. C. M., Podola, B., Melkonian, M. 2005 The 96-well twin-layer system: a novel approach in the cultivation of microalgae. Protist, 156, 239-251.
Keywords: cultivation; culture collections; cell immobilisation; microalgae; microtiter plates; filter plates
Strain(s): 255483485 
PubMed: 16171190
DOI: 10.1016/j.protis.2005.04.003

Suda, S., Watanabe, M. M., Inouye, I. 2004 Electron microscopy of sexual reproduction in Nephroselmis olivacea (Prasinophyceae, Chlorophyta). Phycol. Res., 52, 273-283.
Keywords: electron microscopy; endoplasmic reticulum; germination; Nephroselmis olivacea; Prasinophyceae; sexual reproduction; zygote formation; zygote wall
Strain(s): 483485 
DOI: 10.1111/j.1440-183.2004.00346.x

Marin, B. & Melkonian, M. 1994 Flagellar hairs in prasinophytes (Chlorophyta): ultrastructure and distribution on the flagellar surface. J. Phycol., 30, 659-678.
Keywords: flagellar hairs; Mamiella; Mantoniella; Nephroselmis; Prasinophyceae; Pseudoscourfieldia; Pterosperma; Pyramimonas; Scherffelia; ultrastructure
Strain(s): 221483485 
DOI: 10.1111/j.0022-3646.1994.00659.x

Suda, S., Watanabe, M. M., Inouye, I. 1989 Evidence for sexual reproduction in the primitive green alga Nephroselmis olivacea (Prasinophyceae). J. Phycol., 25, 596-600.
Strain(s): 483485 
DOI: 10.1111/j.1529-8817.1989.tb00266.x

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