| Strain number | NIES-4696 | |||
|---|---|---|---|---|
| Phylum | Rhodophyta | |||
| Class | Cyanidiophyceae | |||
| Scientific name | Cyanidioschyzon merolae De Luca, Taddei & Varano | |||
| Synonym | ||||
| Former name | ||||
| Common name | Red alga | |||
| Locality (Date of collection) | Sardinia Isl., Italy (locality of wild type strain (NIES-1332)) | |||
| Latitude / Longitude | ||||
| Habitat (Isolation source) | Hot spring (NIES-3377) | |||
| History | ||||
| Isolator (Date of isolation) | Suzuki, Shigekatsu (2024-12-**) | |||
| Identified by | ||||
| State of strain | Subculture; Unialgal; Clonal; Axenic[2025 Feb] | |||
|
Culture condition (Preculture condition) |
Medium:
M-Allen
Temperature: 37 C Light intensity: 10-11 µmol photons/m2/sec, L/D cycle: 10L:14D Duration: 6 M |
|||
| Gene information | Nuclear DNA (chromosome 1-20) ( AP006483-AP006502 ) , Mitochondrial DNA ( D8986 ) , Plastid DNA ( AB002583 ) | |||
| Cell size (min - max) | ||||
| Organization | Unicellular | |||
| Characteristics | Thermophilic (opt. 40-45℃（) ; Acidophilic (pH 1.5-2.4) ; Genome decoded strain | |||
| Other strain no. | Other strain no. : 10D | |||
| Remarks | NIES-4696 is available.; Purified strain from NIES-3377; Axenic | |||
| Movie | ||||
| Reference |
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Mogi, Y., Matsuo, Y., Kondo, Y., Higashiyama, T., Inada, T., Yoshida, Y.
2025
Genome-wide changes of protein translation levels for cell and organelle proliferation in a simple unicellular alga.
Proc. Jpn. Acad. Ser. B-Phys. Biol. Sci.,
101,
41-53.
Keywords: cell and organelle division; ribosome profiling; mitochondrial nucleoid protein; mitochondrial division protein; Cyanidioschyzon merolae; eukaryote evolvability Strain(s): 3377, 4696 PubMed: 39805589 DOI: 10.2183/pjab.101.002 Hirata, R., Mogi, Y., Takahashi, K., Nozaki, H., Higashiyama, Y., Yoshida, Y. 2024 Simple prerequisite of presequence for mitochondrial protein import in the unicellular red alga Cyanidioschyzon merolae. J. Cell Sci., 137, jcs262042 (article ID). Keywords: Mitochondrial presequence; Mitochondrial protein import; Monomitochondrial eukaryote; Unicellular red alga Strain(s): 3377, 4696 PubMed: 38940185 DOI: 10.1242/jcs.262042 Yoshida, C., Akiyama, Y., Iwazumi, K., Osanai, T., Ito, S. 2024 L-Lactate production from carbon dioxide in the red alga Cyanidioschyzon merolae. Algal Res., 80, 103526 (article ID). Keywords: Anaerobic cultivation; Cyanidioschyzon merolae; Fermentation; Lactate; Microalgae Strain(s): 3377, 4696 DOI: 10.1016/j.algal.2024.103526 Fujiwara, T., Hirooka, S., Yamashita, S., Yagisawa, F., Miyagishima, S. 2024 Development of a rapamycin-inducible protein-knockdown system in the unicellular red alga Cyanidioschyzon merolae. Plant Physiol., 196, 77–94. Strain(s): 3377, 4696 PubMed: 38833589 DOI: 10.1093/plphys/kiae316 Marchetto, F., Santaeufemia, S., Lebiedzińska-Arciszewska, M., Śliwińska, M. A., Pich, M., Kurek, E., Nazieblo, A., Strawski, M., Solymosi, D., Szklarczyk, M., Bulska, E., Szymański, J., Wierzbicka, M., Allahverdiyeva, Y., Wieckowski, M. R., Kargul, J. 2024 Dynamic adaptation of the extremophilic red microalga Cyanidioschyzon merolae to high nickel stress. Plant Physiol. Biochem., 207, 108365 (article ID). Keywords: Abiotic stress; Adaptation; Cyanidioschyzon merolae; Heavy metals; Nickel; Photosynthesis and respiration Strain(s): 2237, 3377, 4696 PubMed: 38266563 DOI: 10.1016/j.plaphy.2024.108365 Seger, M., Mammadova, F., Villegas-Valencia, M., de Freitas, B. B., Chang, C. R. S., Isachsen, I., Hemstreet, H., Abualsaud, F., Boring, M., Lammers, P. J., Lauersen, K. J. 2023 Engineered ketocarotenoid biosynthesis in the polyextremophilic red microalga Cyanidioschyzon merolae 10D. Metab. Eng. Commun., 17, e00226 (article ID). Keywords: Microalgae; red algae; ketocarotenoids; Cyanidiophyceae; polyextremophiles; astaxanthin Strain(s): 3377, 4696 PubMed: 37449053 DOI: 10.1016/j.mec.2023.e00226 Hamana, K., Furuchi, T., Hayashi, H., Uemura, T., Niitsu, M. 2023 Additional polyamine analysis of the algae belonging to the phyla Glaucophyta, Rhodophyta and Chlorophyta. ─Polyamine analysis of algae V─ Microb. Resour. Syst., 39, 33-40. Keywords: alga; Chlorophyta; Glaucophyta; polyamine; Rhodophyta Strain(s): 226, 250, 254, 550, 551, 626, 1298, 1332, 1703, 1836, 2137, 2163, 2164, 2165, 2166, 2252, 2353, 2894, 3377, 3645, 3867, 3891, 3892, 4107, 4390, 4452, 4696 Wong, D. K., Grisdale, C. J., Slat, V. A., Rader, S. D., Fast, N. M. 2023 The evolution of pre-mRNA splicing and its machinery revealed by reduced extremophilic red algae. J. Eukaryot. Microbiol., 70, e12927 (article ID). Keywords: Cyanidiales; intron; spliceosome; transcriptome Strain(s): 1332, 4696 PubMed: 35662328 DOI: 10.1111/jeu.12927 Chiang, Y.-H., Huang, Y.-J., Fu, H.-Y. 2022 Identifcation of multiple nonphotochemical quenching processes in the extremophilic red alga Cyanidioschyzon merolae. Photosynth. Res., 154, 125–141. Keywords: Energy spillover; Energetic decoupling; Functional antenna size Strain(s): 3377, 4696 PubMed: 36155877 DOI: 10.1007/s11120-022-00963-2 Miyagishimi, S., Tanaka, K. 2021 The unicellular red alga Cyanidioschyzon merolae—The simplest model of a photosynthetic eukaryote. Plant Cell Physiol., 62, 926–941. Keywords: Cyanidiales; Cyanidioschyzon merolae; Photosynthetic eukaryotes; Red algae Strain(s): 3377, 4696 PubMed: 33836072 DOI: 10.1093/pcp/pcab052 Yoshida, C., Murakami, M., Niwa, A., Takeya, M., Osanai, T. 2021 Efficient extraction and preservation of thermotolerant phycocyanins from red alga Cyanidioschyzon merolae. J. Biosci. Bioeng., 131, 161-167. Keywords: Cyanidioschyzon merolae; Microalgae; Phycocyanin; Protein extraction; Red algae Strain(s): 3377, 4696 PubMed: 33109478 DOI: 10.1016/j.jbiosc.2020.09.021 Liu, S.-L., Chiang, Y.-R., Yoon, H. S., Fu, H.-Y. 2020 Comparative genome analysis reveals Cyanidiococcus gen. nov., a new extremophilic red algal genus sister to Cyanidioschyzon (Cyanidioschyzonaceae, Rhodophyta). J. Phycol., 56, 1428-1442. Strain(s): 3377, 4390, 4696 DOI: 10.1111/jpy.13056 Szewczyk, S., Abram, M., Białek, R., Haniewicz, P., Karolczak, J., Gapiński, J., Kargul, J., Gibasiewicz, K. 2020 On the nature of uncoupled chlorophylls in the extremophilic photosystem I-light harvesting I supercomplex. Biochim. Biophys. Acta-Bioenerg., 1861, 148136 (article ID). Keywords: Biosolar photovoltaic cells; Photosystem I; Light harvesting complex I; Uncoupled chlorophylls; Time-resolved fluorescence; Fluorescence correlation spectroscopy Strain(s): 1332, 4696 PubMed: 31825811 DOI: 10.1016/j.bbabio.2019.148136 Abram, M., Białek, R., Szewczyk, S., Karolczak, J., Gibasiewicz, K., Kargul, J. 2020 Remodeling of excitation energy transfer in extremophilic red algal PSI-LHCI complex during light adaptation. Biochim. Biophys. Acta-Bioenerg., 1861, 148093 (article ID). Keywords: Photosystem I; Light harvesting complex I; Time-resolved fluorescence; Extremophilic red alga Cyanidioschyzon merolae; Light adaptation; Red chlorophylls Strain(s): 1332, 4696 PubMed: 31669460 DOI: 10.1016/j.bbabio.2019.148093 Zienkiewicz, M., Krupnik, T., Drożak, A., Kania, K. 2019 PEG-mediated, stable, nuclear and chloroplast transformation of Cyanidioschizon merolae. Bio-protocol, 9, e3355 (article ID). Strain(s): 1332, 3377, 4696 DOI: 10.21769/BioProtoc.3355 Krupnik, T., Wasilewska, W., Drożak, A., Romanowska, E., Zienkiewicz, M. 2018 Application of chloroplast promoters of Cyanidioschyzon merolae for exogenous protein expression. Algae, 33, 351-358. Keywords: chloroplast promoters; chloroplast transformation; Cyanidioschyzon merolae Strain(s): 1332, 4696 DOI: 10.4490/algae.2018.33.12.5 Zienkiewicz, M., Krupnik, T., Drożak, A., Golke, A., Romanowska, E. 2017 Transformation of the Cyanidioschyzon merolae chloroplast genome: prospects for understanding chloroplast function in extreme environments. Plant Mol. Biol., 93, 171-183. Keywords: Stable chloroplast transformation; Cyanidioschyzon merolae; Chloramphenicol acetyltransferase; PEG; Biolistic bombardment Strain(s): 1332, 4696 PubMed: 27796719 DOI: 10.1007/s11103-016-0554-8 Rahman, D. Y, Sarian, F. D., van WijkM, A., Martinez-Garcia, M., van der Maarel, M. J. E. C. 2017 Thermostable phycocyanin from the red microalga Cyanidioschyzon merolae, a new natural blue food colorant. J. Appl. Phycol., 29, 1233–1239. Keywords: Food colorant; Phycocyanin; Pigments; Red microalga; Cyanidioschyzon merolae Strain(s): 1332, 4696 PubMed: 28572707 DOI: 10.1007/s10811-016-1007-0 Loganathan, N., Tsai, YC., Mueller-Cajar, O. 2016 Characterization of the heterooligomeric red-type rubisco activase from red algae. Proc. Nati. Acad. Sci. U. S. A., 113, 14019-14024. Keywords: rubisco; activase; photosynthesis; AAA+ proteins; red algae Strain(s): 1332, 4696 PubMed: 27872295 DOI: 10.1073/pnas.1610758113 Takaichi, S., Yokoyama, A., Mochimaru, M., Uchida, H., Murakami, A. 2016 Carotenogenesis diversification in phylogenetic lineages of Rhodophyta. J. Phycol., 52, 329-338. Keywords: alloxanthin; antheraxanthin; carotenogenesis; carotenoid; Cryptophyta; Glaucophyta; lutein; red algae; Rhodophyta; Zeaxanthin Strain(s): 697, 700, 705, 1332, 2137, 2138, 4696 PubMed: 27273528 DOI: 10.1111/jpy.12411 Krupnik, T., Kotabová, E., van Bezouwen, L. S., Mazur, R., Garstka, M., Nixon, P. J., Barber, J., Kaňa, R., Boekema, E. J., Kargul, J. 2013 A reaction center-dependent photoprotection mechanism in a highly robust photosystem II from an extremophilic red alga, Cyanidioschyzon merolae. J. Biol. Chem., 288, 23529-23542. Strain(s): 1332, 4696 DOI: 10.1074/jbc.M113.484659 Miyagishima, S., Suzuki, K., Okazaki, K., Kabeya, Y. 2012 Expression of the nucleus-encoded chloroplast division genes and proteins regulated by the algal cell cycle. Mol. Biol. Evol., 29, 2957-2970. Keywords: cell cycle; chloroplast division; endosymbiosis; endosymbiotic gene transfer Strain(s): 296, 484, 547, 1332, 2170, 3377, 4696 PubMed: 22490821 DOI: 10.1093/molbev/mss102 Takaichi, S. 2011 Carotenoids in algae: distributions, biosyntheses and functions. Mar. Drugs, 9, 1101-1118. Keywords: algal phylogeny; biosynthesis of carotenoids; distribution of carotenoids; function of carotenoids; pigment-protein complex Strain(s): 144, 1332, 3377, 4696 PubMed: 21747749 DOI: 10.3390/md9061101 |
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