Strain number NIES-4558  
Phylum Dinophyta  
Class Dinophyceae  
Scientific name Prorocentrum lima (Ehrenberg) F.Stein  
Synonym Cryptomonas lima Ehrenberg 1860; Exuviaella lima (Ehrenberg) Bütschli 1885; Exuviaella marina var. lima (Ehrenberg) Schiller 1931; Prorocentrum marinum var. lima (Ehrenberg) Krachmalny 1993  
Former name  
Common name Dinoflagellate  
Locality (Date of collection) Ishikawa, Motobu Okinawa, Japan (2015-02-22)  
Latitude / Longitude 26.695278 / 127.876667 
Habitat (Isolation source) Marine (Seawater)  
History < Adachi, Masao < Nishimura, Tomohiro  
Isolator (Date of isolation) Nishimura, Tomohiro (2015-03-07)  
Identified by Nishimura, Tomohiro; Adachi, Masao  
State of strain Subculture; Unialgal; Clonal; Non-axenic  
Culture condition
(Preculture condition)
Medium:  f/2  
Temperature:  22 C
Light intensity:  24 µmol photons/m2/sec, L/D cycle:  10L:14D
Duration:  45 D  
Gene information 28S rRNA ( LC415595 )  
Cell size (min - max) 30 - 50 μm  
Organization Unicellular; Flagellate 
Characteristics Toxicity ; okadaic acid (+) ; dinophysistoxin-1 (+) ; Benthic ; Attached  
Other strain no. Other strain no. : OMI29P  
Remarks Fragile species to transportation stresses; Read and agree "How to order 4.1"; Toxicity 
Hashimoto, K., Uchida, H., Nishimura, T., Oikawa, H., Funaki, H., Honma, C., Yamaguchi, H., Suzuki, T., Adachi, M. 2021 Determination of optimal culture conditions for toxin production by a Prorocentrum limaHarmful Algae, 103, 102025 (article ID).
Strain(s): 4558 
PubMed: 33980453
DOI: 10.1016/j.hal.2021.102025

Nishimura, T., Uchida, H., Noguchi, R., Oikawa, H., Suzuki, T., Funaki, H., Ihara, C., Hagino, K., Arimitsu, S., Tanii, Y., Abe, S., Hashimoto, K., Mimura, K., Tanaka, K., Yanagida, I., Adachi, M. 2020 Abundance of the benthic dinoflagellate Prorocentrum and the diversity, distribution, and diarrhetic shellfish toxin production of Prorocentrum lima complex and P. caipirignum in Japan. Harmful Algae, 96, 101687 (article ID).
Keywords: Dinoflagellate; Dinophysistoxin-1; Okadaic acid; Phylogeny; Prorocentrum caipirignum; Prorocentrum lima complex
Strain(s): 4554455545574558 
PubMed: 32560839
DOI: 10.1016/j.hal.2019.101687

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