Strain Data

Strain number		NIES-89
Phylum		Cyanophyta
Class		Cyanophyceae
Scientific name		Microcystis aeruginosa (Kützing) Lemmermann
Synonym		Microcystis ichthyoblabe Kützing; Microcystis novacekii (Komárek) Compère; Microcystis viridis (A.Brown) Lemmermann; Microcystis wesenbergii Komárek
Former name		Microcystis aeruginosa (Kützing) Lemmermann f. aeruginosa
Common name		Blue-green alga ; Cyanobacteria
Locality (Date of collection)		Lake Kawaguchi, Yamanashi, Japan (1981-06-**)
Latitude / Longitude		35.5130910551 / 138.748515637
Habitat (Isolation source)		Freshwater (Lake water)
History		< Watanabe, Makoto M.
Isolator (Date of isolation)		Watanabe, Michiko H. (1981-06-**)
Identified by		Watanabe, Michiko H.
State of strain		Cryopreservation; Unialgal; Clonal; Non-axenic
Culture condition (Preculture condition)		Medium: MA Temperature: 25 C Light intensity: 20-30 µmol photons/m²/sec, L/D cycle: 10L:14D Duration: 1 M
Gene information		16S rRNA ( U03403 ) , 16S-23S-ITS ( AB254439 ) , 16S rRNA, tRNA-Ile ( AM773539 ) , ftsZ ( AB324853 ) , glnA ( AB324932 ) , gltX ( AB325011 ) , gyrB ( AB325090 ) , mcnC, mcnE ( AB481218 AM773667 ) , mcyA ( AB110104 AJ515459 ) , mcyD ( AB110115 AY424988 ) , mcyE ( AY382530 ) , mcyG ( AB110126 ) , mcyJ ( AB110137 ) , mdnB ( FN668541 ) , cpcB, cpcA ( AB110149 ) , pgi ( AB325169 ) , recA ( AB325248 ) , tpi ( AB325327 ) , promoter region between mcyA and mcyD ( AB254422 )
Cell size (min - max)
Organization
Characteristics		Cyanobacterial water bloom (aoko) ; Toxicity ; microcystin (+) (Tanabe et al. 2009b) ; ST4 (Tanabe et al. 2007)
Other strain no.		Other strain no. : KW-MA2-5
Remarks		Cryopreserved; Toxicity
Movie

Reference
Liu, J., Zhang, M., Huang, Z., Fang, J., Wang, Z., Zhou, C., Qiu, X. 2023 Diversity, biosynthesis and bioactivity of aeruginosins, a family of cyanobacteria-derived nonribosomal linear tetrapeptides. Mar. Drugs, 21, 217 (article ID). Keywords: aeruginosins; Choi; biogenesis; structural diversity; nonribosomal polypeptide synthesis; serine protease inhibitory activity Strain(s): 89, 98, 101, 205, 298 PubMed: 37103356 DOI: 10.3390/md21040217 Chia, M. A., Ameh, I., George, K. C., Balogun, E. O., Akinyemi, S. A., Lorenzi, A. S. 2022 Genetic diversity of microcystin producers (cyanobacteria) and microcystin congeners in aquatic resources across Africa: A review paper. Toxins, 10, 772 (article ID). Keywords: biological risk; cyanobacteria; cyanotoxin; water supply; African continent; molecular markers; mcy genes Strain(s): 89, 102, 298, 843 PubMed: 36548605 DOI: 10.3390/toxics10120772 Asukabe, H., Akahori, S., Ueno, E., Nakayama, T., Yamashita, R., Arii, S., Harada, K., Imanishi, S. Y. 2020 Cyanobacterial classification with the toxicity using MALDI Biotyper. J. Am. Soc. Mass Spectrom., 31, 1572-1578. Keywords: cyanobacteria; MALDI Biotyper; microcystins; profiling Strain(s): 27, 39, 42, 73, 81, 87, 89, 100, 101, 102, 103, 104, 105, 107, 111, 112, 207, 208, 230, 266, 298, 416, 433, 478, 510, 512, 515, 592, 593, 604, 611, 808, 809, 835, 843, 846, 847, 905, 928, 931, 932, 945, 981, 989, 1025, 1043, 1058, 1061, 1064, 1068, 1069, 1071, 1076, 1085, 1099, 1101, 1105, 1113, 1115, 1126, 1133, 1134, 1135, 1144, 1157, 1215, 1642, 1643, 1652, 1668, 1672, 1673, 1677, 1678, 1698, 2087, 2131, 2412, 2665, 3708, 3733, 3734, 3736, 3755, 3756, 3757 PubMed: 32501712 DOI: 10.1021/jasms.0c00148 Tanabe, Y., Hodoki, Y., Sano, T., Tada, K., Watanabe, MM. 2018a Adaptation of the freshwater bloom-forming cyanobacterium Microcystis aeruginosa to brackish water is driven by recent horizontal transfer of sucrose genes. Front. Microbiol., 9, 1150 (article ID). Keywords: Microcystis; bloom; salt tolerance; sucrose; genomics; brackish water; horizontal gene trasnfer; ecotype Strain(s): 44, 87, 88, 89, 90, 91, 98, 99, 100, 101, 102, 103, 104, 105, 107, 108, 109, 112, 298, 299, 478, 604, 843, 901, 1050, 1052, 1059, 1063, 1067, 1069, 1070, 1075, 1076, 1085, 1093, 1094, 1098, 1100, 1101, 1102, 1104, 1105, 1106, 1107, 1108, 1113, 1114, 1115, 1122, 1128, 1130, 1131, 1132, 1134, 1139, 1141, 1142, 1143, 1144, 1150, 1170, 1174, 1175, 1176, 1177, 1179, 1181, 1183, 1185, 1211, 1213, 1214, 1215, 1232, 1234, 1238, 1239, 1252, 2088, 2090, 2091, 2465, 2466, 2467, 2468, 2469, 2470, 2471, 2472, 2473, 2475, 2476, 2477, 2478, 2479, 2480, 2481, 2482, 2483, 2484, 2485, 2486, 2487, 2488, 2489, 2490, 2491, 2492, 2494, 2495, 2546, 2547, 2548, 2549, 2550, 2551, 2552, 2553, 2554, 2555, 2556, 2557, 2558, 2559, 2560, 2561, 2592, 2594, 2595, 2596, 2597, 2598, 2599, 2600, 2601, 2602, 2603, 2604, 2605, 2606, 2607, 2608, 2610, 2611, 2612, 2613, 2617, 2618, 2619, 2620, 2621, 2622, 2623, 2624, 2625, 2626, 2628, 2629, 4234 PubMed: 29922255 DOI: 10.3389/fmicb.2018.01150 Imanishi, S. Y., Nakayama, T., Asukabe, H., Harada, K. 2017 Application of MALDI Biotyper to cyanobacterial profiling. Rapid Commun. Mass Spectrom., 31, 325-332. Strain(s): 73, 89, 102, 103, 107, 112, 298, 611, 808, 809, 835, 843, 989, 1025, 1043, 1058, 1071, 1099, 1642, 1652, 1672 PubMed: 27862451 DOI: 10.1002/rcm.7793 Anas, A. R. J., Nakajima, A., Naruse, C., Tone, M., Asukabe, H., Harada, K. 2016 Determination of fVIIa-sTF inhibitors in toxic Microcystis cyanobacteria by LC-MS technique. Mar. Drugs, 14, 7 (article ID). Keywords: cyanobacteria; toxic Microcystis; anticoagulant; fVIIa-sTF inhibitors; peptides; aeruginosins; blood coagulation cascade Strain(s): 89, 102, 103, 107, 298, 1025, 1043, 1058, 1071, 1085, 1099, 1133 PubMed: 26729138 DOI: 10.3390/md14010007 Sun, L.W., Jiang, W.J., Sato, H., Kawachi, M., Lu, X.W. 2016 Rapid classification and identification of Microcystis aeruginosa strains using MALDI-TOF MS and polygenetic analysis. PLoS One, 11, e0156275 (article ID). Strain(s): 44, 87, 88, 89, 90, 100, 101, 104, 105, 107, 109, 111, 112, 478, 604, 843, 901, 1043, 1053, 1058, 1061, 1068, 1069, 1070, 1072, 1076, 1077, 1085, 1094, 1099, 1101, 1104, 1105, 1113, 1115, 1126, 1134, 1135, 1144, 1151, 1157, 1170, 1211, 1213, 1215, 1216, 1217, 1218, 1222, 1224, 1230, 1232, 1233, 1239 PubMed: 27227555 DOI: 10.1371/journal.pone.0156275 Tanabe, Y. & Watanabe, M. M. 2011 Local expansion of a panmictic lineage of water bloom-forming cyanobacterium Microcystis aeruginosa. PLoS One, 6, e17085 (article ID). Strain(s): 44, 87, 88, 89, 90, 91, 98, 99, 100, 101, 102, 103, 104, 105, 106, 107, 108, 109, 110, 111, 112, 298, 299, 478, 604, 843, 901, 1050, 1051, 1052, 1053, 1054, 1055, 1056, 1057, 1058, 1059, 1060, 1061, 1062, 1063, 1064, 1065, 1066, 1067, 1068, 1069, 1070, 1072, 1075, 1076, 1077, 1085, 1090, 1091, 1092, 1093, 1094, 1095, 1096, 1097, 1098, 1100, 1101, 1102, 1103, 1104, 1105, 1106, 1107, 1108, 1113, 1114, 1115, 1122, 1123, 1124, 1125, 1126, 1127, 1128, 1129, 1130, 1131, 1132, 1133, 1134, 1135, 1136, 1137, 1138, 1139, 1140, 1141, 1142, 1143, 1144, 1145, 1146, 1147, 1148, 1149, 1150, 1151, 1152, 1153, 1155, 1156, 1157, 1159, 1160, 1161, 1162, 1163, 1164, 1170, 1171, 1172, 1173, 1174, 1175, 1176, 1177, 1179, 1180, 1181, 1183, 1184, 1185, 1186, 1209, 1211, 1213, 1214, 1215, 1216, 1217, 1218, 1219, 1220, 1221, 1222, 1223, 1224, 1225, 1226, 1227, 1228, 1229, 1230, 1231, 1232, 1233, 1234, 1235, 1236, 1237, 1238, 1239, 1240, 1241, 1242, 1243, 1244, 1245, 1251, 1252, 1253, 1254, 2088, 2089, 2090, 2091, 2465, 2466, 2467, 2468, 2469, 2470, 2471, 2472, 2473, 2474, 2475, 2476, 2477, 2478, 2479, 2480, 2481, 2482, 2483, 2484, 2485, 2486, 2487, 2488, 2489, 2490, 2491, 2492, 2493, 2494, 2495, 2496, 2542, 2543, 2544, 2545, 2546, 2547, 2548, 2549, 2550, 2551, 2552, 2553, 2554, 2555, 2556, 2557, 2558, 2559, 2560, 2561, 2591, 2592, 2593, 2594, 2595, 2596, 2597, 2598, 2599, 2600, 2601, 2602, 2603, 2604, 2605, 2606, 2607, 2608, 2609, 2610, 2611, 2612, 2613, 2617, 2618, 2619, 2620, 2621, 2622, 2623, 2624, 2625, 2626, 2627, 2628, 2629 PubMed: 21390221 DOI: 10.1371/journal.pone.0017085 Tanabe, Y., Sano, T., Kasai, F., Watanabe, M. M. 2009b Recombination, cryptic clades and neutral molecular divergence of the microcystin synthetase (mcy) genes of toxic cyanobacterium Microcystis aeruginosa. BMC Evol. Biol., 9, 115 (article ID). Strain(s): 44, 87, 88, 89, 90, 91, 98, 99, 100, 101, 102, 103, 104, 105, 106, 107, 108, 109, 110, 111, 112, 298, 299, 478, 604, 843, 901, 1050, 1051, 1052, 1053, 1054, 1055, 1056, 1057, 1058, 1059, 1060, 1061, 1062, 1063, 1064, 1065, 1066, 1067, 1068, 1069, 1070, 1072, 1075, 1076, 1077, 1085, 1090, 1091, 1092, 1093, 1094, 1095, 1096, 1097, 1098, 1100, 1101, 1102, 1103, 1104, 1105, 1106, 1107, 1108, 1113, 1114, 1115, 1122, 1123, 1124, 1125, 1126, 1127, 1128, 1129, 1130, 1131, 1132, 1133, 1134, 1135, 1136, 1137, 1138, 1139, 1140, 1141, 1142, 1143, 1144, 1145, 1146, 1147, 1148, 1149, 1150, 1151, 1152, 1153, 1155, 1156, 1157, 1159, 1160, 1161, 1162, 1163, 1164, 1170, 1171, 1172, 1209, 1211, 1213, 1214, 1215, 1216, 1217, 1218, 1219, 1220, 1221, 1222, 1223, 1224, 1225, 1226, 1227, 1228, 1229, 1230, 1231, 1232, 1233, 1234, 1235, 1236, 1237, 1238, 1239, 1240, 1241, 1242, 1243, 1244, 1245, 1251, 1252, 1253, 1254, 2465, 2466, 2467, 2469, 2470, 2471, 2472, 2473, 2474, 2475, 2476, 2477, 2478, 2479, 2561 PubMed: 19463155 DOI: 10.1186/1471-2148-9-115 Ha, K., Takamura, N., Jang, M.-H. 2009 Microcystin production by Microcystis aeruginosa exposed to phytoplanktivorous and omnivorous fish at different kairomone concentrations. Bull. Environ. Contam. Toxicol., 83, 761-765. Keywords: microcystin; kairomone; Hypophthalmichthys molitrix; Carassius gibelio langsdorfi Strain(s): 89, 103, 107 PubMed: 19669611 DOI: 10.1007/s00128-009-9841-2 Cadel-Six, S., Dauga, C., Castets, A. M., Rippka, R., Bouchier, C., de Marsac, N. T., Welker, M. 2008 Halogenase genes in nonribosomal peptide synthetase gene clusters of Microcystis (Cyanobacteria): sporadic distribution and evolution. Mol. Biol. Evol., 25, 2031-2041. Keywords: halogenase; cyanopeptolin; aeruginosin; DNA rearrangement; secondary peptide metabolite; chlorination; internal transcribed spacer; phylogeny Strain(s): 89, 98, 101, 102, 298 PubMed: 18614525 DOI: 10.1093/molbev/msn150 Tanabe, Y., Kasai, F., Watanabe, M. M. 2007 Multilocus sequence typing (MLST) reveals high genetic diversity and clonal population structure of the toxic cyanobacterium Microcystis aeruginosa. Microbiology, 153, 3695-3703. Strain(s): 44, 87, 88, 89, 90, 91, 98, 99, 100, 101, 102, 103, 104, 105, 106, 107, 108, 109, 110, 111, 112, 298, 299, 478, 604, 843, 901, 933, 1050, 1051, 1052, 1053, 1054, 1055, 1056, 1057, 1058, 1059, 1060, 1061, 1062, 1063, 1064, 1065, 1066, 1067, 1068, 1069, 1070, 1072, 1075, 1076, 1077, 1085, 1090, 1091, 1092, 1093, 1094, 1095, 1096, 1097, 1098, 1100, 1101, 1102, 1103, 1104, 1105, 1106, 1107, 1108, 1113, 1114, 1115, 1122, 1123, 1124, 1125, 1126, 1127, 1128, 1129, 1130, 1131, 1132, 1133, 1134, 1135, 1136, 1137, 1138, 1139, 1140, 1141, 1142, 1143, 1144, 1145, 1146, 1147, 1148, 1149, 1150, 1151, 1152, 1153, 1155, 1156, 1157, 1159, 1160, 1161, 1162, 1163, 1164, 1170, 1171, 1172, 1209, 1211, 1213, 1214, 1215, 1216, 1217, 1218, 1219, 1220, 1221, 1222, 1223, 1224, 1225, 1226, 1227, 1228, 1229, 1230, 1231, 1232, 1233, 1234, 1235, 1236, 1237, 1238, 1239, 1240, 1241, 1242, 1243, 1244, 1245, 1251, 1252, 1253, 1254 PubMed: 17975077 DOI: 10.1099/mic.0.2007/010645-0 Koskenniemi, K., Lyra, C., Rajaniemi-Wacklin, P., Jokela, J., Sivonen, K. 2007 Quantitative real-time PCR detection of toxic Nodularia cyanobacteria in the Baltic Sea. Appl. Environ. Microbiol., 73, 2173-2179. Strain(s): 81, 89, 102 PubMed: 17277219 DOI: 10.1128/AEM.02746-06 Tanabe, Y., Kaya, K., Watanabe, M. M. 2004 Evidence for recombination in the microcystin synthetase (mcy) genes of toxic cyanobacteria Microcystis spp. J. Mol. Evol., 58, 633-641. Keywords: cyanobacteria; microcystin synthetase; Microcystis; NRPS; recombination; split decomposition Strain(s): 88, 89, 90, 102, 103, 107 PubMed: 15461420 DOI: 10.1007/s00239-004-2583-1 Yoshida, T., Yuki, Y., Lei, S., Chinen, H., Yoshida, M., Kondo, R., Hiroishi, S. 2003 Quantitative detection of toxic strains of the cyanobacterial genus Microcystis by competitive PCR. Microbes Environ., 18, 16-23. Keywords: competitive PCR; microcystin; Microcystis; peptide synthetase; toxic cyanobacteria Strain(s): 87, 88, 89, 90, 102, 104, 105, 106, 108, 109, 110, 111, 112, 298, 299, 604 DOI: 10.1264/jsme2.18.16 Mori, F., Erata, M., Watanabe, M. M. 2002 Cryopreservation of cyanobacteria and green algae in the NIES-Collection. Microbiol. Cult. Coll., 18, 45-55. Keywords: cryopreservation; cyanobacteria; green algae; FDA staining method; NIES-Collection Strain(s): 19, 21, 22, 23, 24, 25, 26, 27, 28, 29, 30, 31, 32, 33, 34, 35, 36, 37, 38, 39, 40, 41, 44, 45, 46, 50, 51, 52, 53, 54, 55, 56, 58, 59, 60, 61, 62, 63, 64, 65, 66, 67, 68, 69, 70, 73, 74, 75, 76, 77, 78, 79, 80, 81, 87, 89, 90, 91, 92, 93, 94, 95, 96, 97, 98, 99, 100, 101, 102, 103, 104, 105, 106, 107, 108, 109, 110, 111, 112, 119, 120, 122, 123, 125, 127, 128, 129, 130, 131, 132, 133, 134, 135, 137, 138, 139, 144, 147, 151, 152, 153, 154, 155, 156, 157, 158, 159, 160, 162, 163, 164, 165, 166, 167, 168, 170, 171, 172, 173, 174, 175, 176, 177, 178, 179, 180, 181, 182, 183, 185, 186, 187, 188, 189, 191, 192, 193, 194, 197, 198, 199, 200, 201, 202, 203, 204, 205, 206, 207, 208, 209, 210, 211, 212, 213, 214, 215, 216, 217, 224, 226, 227, 228, 229, 230, 231, 232, 241, 242, 243, 244, 245, 246, 248, 257, 259, 261, 263, 266, 267, 268, 287, 288, 289, 290, 294, 295, 297, 298, 299, 300, 301, 302, 303, 305, 306, 308, 309, 310, 312, 313, 329, 334, 337, 338, 339, 340, 341, 342, 349, 351, 359, 360, 361, 362, 375, 378, 379, 380, 382, 384, 385, 390, 394, 396, 397, 398, 415, 416, 418, 419, 421, 422, 423, 424, 425, 426, 427, 428, 429, 430, 431, 432, 433, 434, 436, 437, 438, 439, 440, 446, 447, 448, 449, 450, 451, 452, 453, 454, 455, 456, 457, 458, 459, 460, 464, 468, 469, 474, 478, 479, 480, 481, 503, 504, 505, 506, 507, 509, 510, 512, 514, 515, 522, 523, 524, 527, 528, 529, 530, 531, 532, 536, 537, 538, 539, 540, 541, 542, 543, 544, 545, 546, 564, 565, 566, 567, 568, 569, 570, 571, 572, 573, 574, 575, 576, 577, 578, 579, 580, 581, 582, 583, 584, 585, 592, 593, 594, 595, 596, 597, 598, 604, 610, 628, 630, 631, 632, 633, 634, 635, 636, 637, 638, 639, 640, 641, 642, 643, 644, 645, 646, 647, 648, 649, 650, 651, 652, 653, 654, 655, 656, 657, 658, 659, 660, 661, 662, 663, 664, 665, 666, 667, 672, 685, 686, 687, 688, 689, 690, 691, 692, 693, 694, 717, 718, 719, 720, 721, 722, 723, 724, 725, 726, 727, 728, 729 Yuki, Y., Yoshida, T., Hiroishi, S. 2002 Molecular detection of the toxic cyanobacteria [Yûdoku aoko no bunshi-shikibetsu to yosatsu he-no ôyô]. In Series of Fisheries Science Vol. 143, Prevention and Extermination Strategies of Harmful Algal Blooms [Suisan-gaku shirîzu 134, Yûgai-yûdoku sôrui burûmu no yobô to kujo] , Eds. by Hiroishi, S., Imai, I. & Ishimaru, T., Koseisha-Koseikaku, Tokyo, pp. 43-53 (in Japanese). Strain(s): 73, 81, 87, 88, 89, 90, 91, 98, 99, 100, 101, 102, 104, 105, 106, 108, 109, 110, 111, 112, 204, 230, 298, 299, 604 Lyra, C., Suomalainen, S., Gugger, M., Vezie, C., Sundman, P., Paulin, L., Sivonen, K. 2001 Molecular characterization of planktic cyanobacteria of Anabaena, Aphanizomenon, Microcystis and Planktothrix genera. Int. J. Syst. Evol. Microbiol., 51, 13-526. Keywords: cyanobacteria; 16S rRNA gene; RFLP; sequencing; REP; ERIC Strain(s): 41, 44, 73, 79, 81, 89, 98, 102, 104 DOI: 10.1099/00207713-51-2-513 Kondo, R., Yoshida, T., Yuki, Y., Hiroishi, S. 2000 DNA-DNA reassociation among a bloom-forming cyanobacterial genus, Microcystis. Int. J. Syst. Evol. Microbiol., 50, 767-770. Keywords: DNA hybridization; DNA G+C content; cyanobacteria; Microcystis Strain(s): 87, 89, 98, 102, 104, 111, 112, 298, 604 PubMed: 10758887 DOI: 10.1099/00207713-50-2-767 Kondo, R., Kagiya, G., Komura, M., Yuki, Y., Hiroishi, S., Watanabe, M. 2000 Molecular taxonomy of a bloom-forming cyanobacterial genus Microcystis. Bull. Plankton Soc. Japan, 47, 25-33 (in Japanese with English summary). Keywords: cyanobacteria; Microcystis; bacterial criteria; molecular analysis Strain(s): 6, 87, 88, 89, 90, 91, 98, 99, 100, 101, 102, 104, 105, 106, 108, 109, 110, 111, 112, 298, 299, 604, 1055, 1061, 1068, 1076, 1090, 1091, 1105, 1122, 1142, 1164 Kondo, R., Kagiya, G., Hiroishi, S., Watanabe, M. 2000 Genetic typing of a bloom-forming cyanobacterial genus Microcystis in Japan using 16S rRNA gene sequence analysis. Plankton Biol. Ecol., 47, 1-6. Keywords: genetic typing; 16S rDNA; Microcystis; cyanobacteria; phylogenetic analysis Strain(s): 87, 88, 89, 90, 91, 98, 99, 100, 101, 102, 104, 105, 106, 108, 109, 110, 111, 112, 298, 299, 604, 1055, 1061, 1068, 1076, 1090, 1091, 1105, 1122, 1142, 1164 Ishida, K., Okita, Y., Matsuda, H., Okino, T., Murakami, M. 1999 Aeruginosins, protease inhibitors from the cyanobacterium Microcystis aeruginosa. Tetrahedron, 55, 10971-10988. Strain(s): 89, 98, 298 DOI: 10.1016/S0040-4020(99)00621-3 Kondo, R., Kagiya, G., Hiroishi, S., Watanabe, M., Hata, Y. 1999 Rapid DNA extraction from a bloom-forming cyanobacterium. Microbes Environ., 14, 157-161. Keywords: DNA extraction; PCR; Microcystis; cyanobacteria Strain(s): 87, 88, 89, 90, 91, 98, 99, 100, 101, 102, 104, 105, 106, 108, 109, 110, 111, 112, 298, 299, 604, 1055, 1061, 1068, 1076, 1090, 1091, 1105, 1122, 1142, 1164 DOI: 10.1264/jsme2.14.157 Li, R., Yokota, A., Sugiyama, J., Watanabe, M., Hiroki, M., Watanabe, M. M. 1998 Chemotaxonomy of planktonic cyanobacteria based on non-polar and 3-hydroxy fatty acid composition. Phycol. Res., 46, 21-28. Keywords: 3-hydroxy fatty acids; chemotaxonomy; fatty acid; non-polar fatty acids; planktonic cyanobacteria Strain(s): 27, 40, 44, 76, 78, 79, 80, 81, 87, 89, 98, 102, 104, 111, 112, 204, 205, 207, 298, 604, 810, 821, 825, 826 DOI: 10.1111/j.1440-1835.1998.tb00093.x Kondo, R., Kagiya, G., Yuki, Y., Hiroishi, S., Watanabe, M. 1998 Taxonomy of a bloom-forming cyanobacterial genus Microcystis. Nippon Suisan Gakkaishi, 64, 291-292 (in Japanese with English title). Strain(s): 87, 88, 89, 90, 91, 98, 99, 100, 101, 102, 104, 105, 106, 107, 108, 109, 110, 111, 112, 298, 299, 604 Neilan, B. A., Jacobs, D., Goodman, A. E. 1995 Genetic diversity and phylogeny of toxic cyanobacteria determined by DNA polymorphisms within the phycocyanin locus. Appl. Environ. Microbiol., 61, 3875-3883. Strain(s): 24, 40, 41, 44, 73, 76, 78, 80, 81, 87, 89, 98, 102, 107, 111, 112, 204, 207, 298 PubMed: 8526499 Neilan, B. A., Hawkins, P. R., Cox, P. T., Goodman, A. E. 1994 Towards a molecular taxonomy for the bloom-forming cyanobacteria. Aust. J. Mar. Freshwater Res., 45, 869-873. Keywords: 16S rRNA; PCR; Microcystis; Anabaena; DNA sequence Strain(s): 41, 78, 89, 102, 107, 111 DOI: 10.1071/MF9940869 Neilan, B. A., Cox, P. T., Hawkins, P. R., Goodman, A. E. 1994 16S ribosomal RNA gene sequence and phylogeny of toxic Microcystis sp. (Cyanobacteria). DNA Seq., 4, 333-337. Keywords: Cyanobacteria; Microcystis; 165 rRNA gene; DNA sequence; taxonomy; phylogeny Strain(s): 89 DOI: 10.3109/10425179409020861 Watanabe, M. M., Suda, S., Kasai, F., Sawaguchi, T. 1985 Axenic cultures of the three species of Microcystis (Cyanophyta = Cyanobacteria). Bull. JFCC, 1, 57-63. Strain(s): 89, 102, 107, 111, 298 Watanabe, M. M., Kasai, F., Hiwatari, T., Suda, S., Nei, T. 1984 Cryopreservation of microalgae in liquid nitrogen - Survival of algal cultures after freezing. Jpn. J. Freez. Dry., 30, 23-26 (in Japanese with English title). Strain(s): 18, 19, 21, 24, 26, 29, 31, 39, 40, 41, 44, 45, 46, 50, 73, 74, 75, 76, 77, 80, 81, 87, 88, 89, 90, 91, 93, 95, 98, 99, 100, 101, 120, 122, 131, 135, 137, 144, 151, 204, 207, 213, 257, 263, 379, 415, 2219, 2235, 2239, 2579

Reference

Liu, J., Zhang, M., Huang, Z., Fang, J., Wang, Z., Zhou, C., Qiu, X. 2023 Diversity, biosynthesis and bioactivity of aeruginosins, a family of cyanobacteria-derived nonribosomal linear tetrapeptides. Mar. Drugs, 21, 217 (article ID).
Keywords: aeruginosins; Choi; biogenesis; structural diversity; nonribosomal polypeptide synthesis; serine protease inhibitory activity
Strain(s): 89, 98, 101, 205, 298
PubMed: 37103356
DOI: 10.3390/md21040217

Chia, M. A., Ameh, I., George, K. C., Balogun, E. O., Akinyemi, S. A., Lorenzi, A. S. 2022 Genetic diversity of microcystin producers (cyanobacteria) and microcystin congeners in aquatic resources across Africa: A review paper. Toxins, 10, 772 (article ID).
Keywords: biological risk; cyanobacteria; cyanotoxin; water supply; African continent; molecular markers; mcy genes
Strain(s): 89, 102, 298, 843
PubMed: 36548605
DOI: 10.3390/toxics10120772

Asukabe, H., Akahori, S., Ueno, E., Nakayama, T., Yamashita, R., Arii, S., Harada, K., Imanishi, S. Y. 2020 Cyanobacterial classification with the toxicity using MALDI Biotyper. J. Am. Soc. Mass Spectrom., 31, 1572-1578.
Keywords: cyanobacteria; MALDI Biotyper; microcystins; profiling
Strain(s): 27, 39, 42, 73, 81, 87, 89, 100, 101, 102, 103, 104, 105, 107, 111, 112, 207, 208, 230, 266, 298, 416, 433, 478, 510, 512, 515, 592, 593, 604, 611, 808, 809, 835, 843, 846, 847, 905, 928, 931, 932, 945, 981, 989, 1025, 1043, 1058, 1061, 1064, 1068, 1069, 1071, 1076, 1085, 1099, 1101, 1105, 1113, 1115, 1126, 1133, 1134, 1135, 1144, 1157, 1215, 1642, 1643, 1652, 1668, 1672, 1673, 1677, 1678, 1698, 2087, 2131, 2412, 2665, 3708, 3733, 3734, 3736, 3755, 3756, 3757
PubMed: 32501712
DOI: 10.1021/jasms.0c00148

Tanabe, Y., Hodoki, Y., Sano, T., Tada, K., Watanabe, MM. 2018a Adaptation of the freshwater bloom-forming cyanobacterium Microcystis aeruginosa to brackish water is driven by recent horizontal transfer of sucrose genes. Front. Microbiol., 9, 1150 (article ID).
Keywords: Microcystis; bloom; salt tolerance; sucrose; genomics; brackish water; horizontal gene trasnfer; ecotype
Strain(s): 44, 87, 88, 89, 90, 91, 98, 99, 100, 101, 102, 103, 104, 105, 107, 108, 109, 112, 298, 299, 478, 604, 843, 901, 1050, 1052, 1059, 1063, 1067, 1069, 1070, 1075, 1076, 1085, 1093, 1094, 1098, 1100, 1101, 1102, 1104, 1105, 1106, 1107, 1108, 1113, 1114, 1115, 1122, 1128, 1130, 1131, 1132, 1134, 1139, 1141, 1142, 1143, 1144, 1150, 1170, 1174, 1175, 1176, 1177, 1179, 1181, 1183, 1185, 1211, 1213, 1214, 1215, 1232, 1234, 1238, 1239, 1252, 2088, 2090, 2091, 2465, 2466, 2467, 2468, 2469, 2470, 2471, 2472, 2473, 2475, 2476, 2477, 2478, 2479, 2480, 2481, 2482, 2483, 2484, 2485, 2486, 2487, 2488, 2489, 2490, 2491, 2492, 2494, 2495, 2546, 2547, 2548, 2549, 2550, 2551, 2552, 2553, 2554, 2555, 2556, 2557, 2558, 2559, 2560, 2561, 2592, 2594, 2595, 2596, 2597, 2598, 2599, 2600, 2601, 2602, 2603, 2604, 2605, 2606, 2607, 2608, 2610, 2611, 2612, 2613, 2617, 2618, 2619, 2620, 2621, 2622, 2623, 2624, 2625, 2626, 2628, 2629, 4234
PubMed: 29922255
DOI: 10.3389/fmicb.2018.01150

Imanishi, S. Y., Nakayama, T., Asukabe, H., Harada, K. 2017 Application of MALDI Biotyper to cyanobacterial profiling. Rapid Commun. Mass Spectrom., 31, 325-332.
Strain(s): 73, 89, 102, 103, 107, 112, 298, 611, 808, 809, 835, 843, 989, 1025, 1043, 1058, 1071, 1099, 1642, 1652, 1672
PubMed: 27862451
DOI: 10.1002/rcm.7793

Anas, A. R. J., Nakajima, A., Naruse, C., Tone, M., Asukabe, H., Harada, K. 2016 Determination of fVIIa-sTF inhibitors in toxic Microcystis cyanobacteria by LC-MS technique. Mar. Drugs, 14, 7 (article ID).
Keywords: cyanobacteria; toxic Microcystis; anticoagulant; fVIIa-sTF inhibitors; peptides; aeruginosins; blood coagulation cascade
Strain(s): 89, 102, 103, 107, 298, 1025, 1043, 1058, 1071, 1085, 1099, 1133
PubMed: 26729138
DOI: 10.3390/md14010007

Sun, L.W., Jiang, W.J., Sato, H., Kawachi, M., Lu, X.W. 2016 Rapid classification and identification of Microcystis aeruginosa strains using MALDI-TOF MS and polygenetic analysis. PLoS One, 11, e0156275 (article ID).
Strain(s): 44, 87, 88, 89, 90, 100, 101, 104, 105, 107, 109, 111, 112, 478, 604, 843, 901, 1043, 1053, 1058, 1061, 1068, 1069, 1070, 1072, 1076, 1077, 1085, 1094, 1099, 1101, 1104, 1105, 1113, 1115, 1126, 1134, 1135, 1144, 1151, 1157, 1170, 1211, 1213, 1215, 1216, 1217, 1218, 1222, 1224, 1230, 1232, 1233, 1239
PubMed: 27227555
DOI: 10.1371/journal.pone.0156275

Tanabe, Y. & Watanabe, M. M. 2011 Local expansion of a panmictic lineage of water bloom-forming cyanobacterium Microcystis aeruginosa. PLoS One, 6, e17085 (article ID).
Strain(s): 44, 87, 88, 89, 90, 91, 98, 99, 100, 101, 102, 103, 104, 105, 106, 107, 108, 109, 110, 111, 112, 298, 299, 478, 604, 843, 901, 1050, 1051, 1052, 1053, 1054, 1055, 1056, 1057, 1058, 1059, 1060, 1061, 1062, 1063, 1064, 1065, 1066, 1067, 1068, 1069, 1070, 1072, 1075, 1076, 1077, 1085, 1090, 1091, 1092, 1093, 1094, 1095, 1096, 1097, 1098, 1100, 1101, 1102, 1103, 1104, 1105, 1106, 1107, 1108, 1113, 1114, 1115, 1122, 1123, 1124, 1125, 1126, 1127, 1128, 1129, 1130, 1131, 1132, 1133, 1134, 1135, 1136, 1137, 1138, 1139, 1140, 1141, 1142, 1143, 1144, 1145, 1146, 1147, 1148, 1149, 1150, 1151, 1152, 1153, 1155, 1156, 1157, 1159, 1160, 1161, 1162, 1163, 1164, 1170, 1171, 1172, 1173, 1174, 1175, 1176, 1177, 1179, 1180, 1181, 1183, 1184, 1185, 1186, 1209, 1211, 1213, 1214, 1215, 1216, 1217, 1218, 1219, 1220, 1221, 1222, 1223, 1224, 1225, 1226, 1227, 1228, 1229, 1230, 1231, 1232, 1233, 1234, 1235, 1236, 1237, 1238, 1239, 1240, 1241, 1242, 1243, 1244, 1245, 1251, 1252, 1253, 1254, 2088, 2089, 2090, 2091, 2465, 2466, 2467, 2468, 2469, 2470, 2471, 2472, 2473, 2474, 2475, 2476, 2477, 2478, 2479, 2480, 2481, 2482, 2483, 2484, 2485, 2486, 2487, 2488, 2489, 2490, 2491, 2492, 2493, 2494, 2495, 2496, 2542, 2543, 2544, 2545, 2546, 2547, 2548, 2549, 2550, 2551, 2552, 2553, 2554, 2555, 2556, 2557, 2558, 2559, 2560, 2561, 2591, 2592, 2593, 2594, 2595, 2596, 2597, 2598, 2599, 2600, 2601, 2602, 2603, 2604, 2605, 2606, 2607, 2608, 2609, 2610, 2611, 2612, 2613, 2617, 2618, 2619, 2620, 2621, 2622, 2623, 2624, 2625, 2626, 2627, 2628, 2629
PubMed: 21390221
DOI: 10.1371/journal.pone.0017085

Tanabe, Y., Sano, T., Kasai, F., Watanabe, M. M. 2009b Recombination, cryptic clades and neutral molecular divergence of the microcystin synthetase (mcy) genes of toxic cyanobacterium Microcystis aeruginosa. BMC Evol. Biol., 9, 115 (article ID).
Strain(s): 44, 87, 88, 89, 90, 91, 98, 99, 100, 101, 102, 103, 104, 105, 106, 107, 108, 109, 110, 111, 112, 298, 299, 478, 604, 843, 901, 1050, 1051, 1052, 1053, 1054, 1055, 1056, 1057, 1058, 1059, 1060, 1061, 1062, 1063, 1064, 1065, 1066, 1067, 1068, 1069, 1070, 1072, 1075, 1076, 1077, 1085, 1090, 1091, 1092, 1093, 1094, 1095, 1096, 1097, 1098, 1100, 1101, 1102, 1103, 1104, 1105, 1106, 1107, 1108, 1113, 1114, 1115, 1122, 1123, 1124, 1125, 1126, 1127, 1128, 1129, 1130, 1131, 1132, 1133, 1134, 1135, 1136, 1137, 1138, 1139, 1140, 1141, 1142, 1143, 1144, 1145, 1146, 1147, 1148, 1149, 1150, 1151, 1152, 1153, 1155, 1156, 1157, 1159, 1160, 1161, 1162, 1163, 1164, 1170, 1171, 1172, 1209, 1211, 1213, 1214, 1215, 1216, 1217, 1218, 1219, 1220, 1221, 1222, 1223, 1224, 1225, 1226, 1227, 1228, 1229, 1230, 1231, 1232, 1233, 1234, 1235, 1236, 1237, 1238, 1239, 1240, 1241, 1242, 1243, 1244, 1245, 1251, 1252, 1253, 1254, 2465, 2466, 2467, 2469, 2470, 2471, 2472, 2473, 2474, 2475, 2476, 2477, 2478, 2479, 2561
PubMed: 19463155
DOI: 10.1186/1471-2148-9-115

Ha, K., Takamura, N., Jang, M.-H. 2009 Microcystin production by Microcystis aeruginosa exposed to phytoplanktivorous and omnivorous fish at different kairomone concentrations. Bull. Environ. Contam. Toxicol., 83, 761-765.
Keywords: microcystin; kairomone; Hypophthalmichthys molitrix; Carassius gibelio langsdorfi
Strain(s): 89, 103, 107
PubMed: 19669611
DOI: 10.1007/s00128-009-9841-2

Cadel-Six, S., Dauga, C., Castets, A. M., Rippka, R., Bouchier, C., de Marsac, N. T., Welker, M. 2008 Halogenase genes in nonribosomal peptide synthetase gene clusters of Microcystis (Cyanobacteria): sporadic distribution and evolution. Mol. Biol. Evol., 25, 2031-2041.
Keywords: halogenase; cyanopeptolin; aeruginosin; DNA rearrangement; secondary peptide metabolite; chlorination; internal transcribed spacer; phylogeny
Strain(s): 89, 98, 101, 102, 298
PubMed: 18614525
DOI: 10.1093/molbev/msn150

Tanabe, Y., Kasai, F., Watanabe, M. M. 2007 Multilocus sequence typing (MLST) reveals high genetic diversity and clonal population structure of the toxic cyanobacterium Microcystis aeruginosa. Microbiology, 153, 3695-3703.
Strain(s): 44, 87, 88, 89, 90, 91, 98, 99, 100, 101, 102, 103, 104, 105, 106, 107, 108, 109, 110, 111, 112, 298, 299, 478, 604, 843, 901, 933, 1050, 1051, 1052, 1053, 1054, 1055, 1056, 1057, 1058, 1059, 1060, 1061, 1062, 1063, 1064, 1065, 1066, 1067, 1068, 1069, 1070, 1072, 1075, 1076, 1077, 1085, 1090, 1091, 1092, 1093, 1094, 1095, 1096, 1097, 1098, 1100, 1101, 1102, 1103, 1104, 1105, 1106, 1107, 1108, 1113, 1114, 1115, 1122, 1123, 1124, 1125, 1126, 1127, 1128, 1129, 1130, 1131, 1132, 1133, 1134, 1135, 1136, 1137, 1138, 1139, 1140, 1141, 1142, 1143, 1144, 1145, 1146, 1147, 1148, 1149, 1150, 1151, 1152, 1153, 1155, 1156, 1157, 1159, 1160, 1161, 1162, 1163, 1164, 1170, 1171, 1172, 1209, 1211, 1213, 1214, 1215, 1216, 1217, 1218, 1219, 1220, 1221, 1222, 1223, 1224, 1225, 1226, 1227, 1228, 1229, 1230, 1231, 1232, 1233, 1234, 1235, 1236, 1237, 1238, 1239, 1240, 1241, 1242, 1243, 1244, 1245, 1251, 1252, 1253, 1254
PubMed: 17975077
DOI: 10.1099/mic.0.2007/010645-0

Koskenniemi, K., Lyra, C., Rajaniemi-Wacklin, P., Jokela, J., Sivonen, K. 2007 Quantitative real-time PCR detection of toxic Nodularia cyanobacteria in the Baltic Sea. Appl. Environ. Microbiol., 73, 2173-2179.
Strain(s): 81, 89, 102
PubMed: 17277219
DOI: 10.1128/AEM.02746-06

Tanabe, Y., Kaya, K., Watanabe, M. M. 2004 Evidence for recombination in the microcystin synthetase (mcy) genes of toxic cyanobacteria Microcystis spp. J. Mol. Evol., 58, 633-641.
Keywords: cyanobacteria; microcystin synthetase; Microcystis; NRPS; recombination; split decomposition
Strain(s): 88, 89, 90, 102, 103, 107
PubMed: 15461420
DOI: 10.1007/s00239-004-2583-1

Yoshida, T., Yuki, Y., Lei, S., Chinen, H., Yoshida, M., Kondo, R., Hiroishi, S. 2003 Quantitative detection of toxic strains of the cyanobacterial genus Microcystis by competitive PCR. Microbes Environ., 18, 16-23.
Keywords: competitive PCR; microcystin; Microcystis; peptide synthetase; toxic cyanobacteria
Strain(s): 87, 88, 89, 90, 102, 104, 105, 106, 108, 109, 110, 111, 112, 298, 299, 604
DOI: 10.1264/jsme2.18.16

Mori, F., Erata, M., Watanabe, M. M. 2002 Cryopreservation of cyanobacteria and green algae in the NIES-Collection. Microbiol. Cult. Coll., 18, 45-55.
Keywords: cryopreservation; cyanobacteria; green algae; FDA staining method; NIES-Collection
Strain(s): 19, 21, 22, 23, 24, 25, 26, 27, 28, 29, 30, 31, 32, 33, 34, 35, 36, 37, 38, 39, 40, 41, 44, 45, 46, 50, 51, 52, 53, 54, 55, 56, 58, 59, 60, 61, 62, 63, 64, 65, 66, 67, 68, 69, 70, 73, 74, 75, 76, 77, 78, 79, 80, 81, 87, 89, 90, 91, 92, 93, 94, 95, 96, 97, 98, 99, 100, 101, 102, 103, 104, 105, 106, 107, 108, 109, 110, 111, 112, 119, 120, 122, 123, 125, 127, 128, 129, 130, 131, 132, 133, 134, 135, 137, 138, 139, 144, 147, 151, 152, 153, 154, 155, 156, 157, 158, 159, 160, 162, 163, 164, 165, 166, 167, 168, 170, 171, 172, 173, 174, 175, 176, 177, 178, 179, 180, 181, 182, 183, 185, 186, 187, 188, 189, 191, 192, 193, 194, 197, 198, 199, 200, 201, 202, 203, 204, 205, 206, 207, 208, 209, 210, 211, 212, 213, 214, 215, 216, 217, 224, 226, 227, 228, 229, 230, 231, 232, 241, 242, 243, 244, 245, 246, 248, 257, 259, 261, 263, 266, 267, 268, 287, 288, 289, 290, 294, 295, 297, 298, 299, 300, 301, 302, 303, 305, 306, 308, 309, 310, 312, 313, 329, 334, 337, 338, 339, 340, 341, 342, 349, 351, 359, 360, 361, 362, 375, 378, 379, 380, 382, 384, 385, 390, 394, 396, 397, 398, 415, 416, 418, 419, 421, 422, 423, 424, 425, 426, 427, 428, 429, 430, 431, 432, 433, 434, 436, 437, 438, 439, 440, 446, 447, 448, 449, 450, 451, 452, 453, 454, 455, 456, 457, 458, 459, 460, 464, 468, 469, 474, 478, 479, 480, 481, 503, 504, 505, 506, 507, 509, 510, 512, 514, 515, 522, 523, 524, 527, 528, 529, 530, 531, 532, 536, 537, 538, 539, 540, 541, 542, 543, 544, 545, 546, 564, 565, 566, 567, 568, 569, 570, 571, 572, 573, 574, 575, 576, 577, 578, 579, 580, 581, 582, 583, 584, 585, 592, 593, 594, 595, 596, 597, 598, 604, 610, 628, 630, 631, 632, 633, 634, 635, 636, 637, 638, 639, 640, 641, 642, 643, 644, 645, 646, 647, 648, 649, 650, 651, 652, 653, 654, 655, 656, 657, 658, 659, 660, 661, 662, 663, 664, 665, 666, 667, 672, 685, 686, 687, 688, 689, 690, 691, 692, 693, 694, 717, 718, 719, 720, 721, 722, 723, 724, 725, 726, 727, 728, 729

Yuki, Y., Yoshida, T., Hiroishi, S. 2002 Molecular detection of the toxic cyanobacteria [Yûdoku aoko no bunshi-shikibetsu to yosatsu he-no ôyô]. In Series of Fisheries Science Vol. 143, Prevention and Extermination Strategies of Harmful Algal Blooms [Suisan-gaku shirîzu 134, Yûgai-yûdoku sôrui burûmu no yobô to kujo] , Eds. by Hiroishi, S., Imai, I. & Ishimaru, T., Koseisha-Koseikaku, Tokyo, pp. 43-53 (in Japanese).
Strain(s): 73, 81, 87, 88, 89, 90, 91, 98, 99, 100, 101, 102, 104, 105, 106, 108, 109, 110, 111, 112, 204, 230, 298, 299, 604

Lyra, C., Suomalainen, S., Gugger, M., Vezie, C., Sundman, P., Paulin, L., Sivonen, K. 2001 Molecular characterization of planktic cyanobacteria of Anabaena, Aphanizomenon, Microcystis and Planktothrix genera. Int. J. Syst. Evol. Microbiol., 51, 13-526.
Keywords: cyanobacteria; 16S rRNA gene; RFLP; sequencing; REP; ERIC
Strain(s): 41, 44, 73, 79, 81, 89, 98, 102, 104
DOI: 10.1099/00207713-51-2-513

Kondo, R., Yoshida, T., Yuki, Y., Hiroishi, S. 2000 DNA-DNA reassociation among a bloom-forming cyanobacterial genus, Microcystis. Int. J. Syst. Evol. Microbiol., 50, 767-770.
Keywords: DNA hybridization; DNA G+C content; cyanobacteria; Microcystis
Strain(s): 87, 89, 98, 102, 104, 111, 112, 298, 604
PubMed: 10758887
DOI: 10.1099/00207713-50-2-767

Kondo, R., Kagiya, G., Komura, M., Yuki, Y., Hiroishi, S., Watanabe, M. 2000 Molecular taxonomy of a bloom-forming cyanobacterial genus Microcystis. Bull. Plankton Soc. Japan, 47, 25-33 (in Japanese with English summary).
Keywords: cyanobacteria; Microcystis; bacterial criteria; molecular analysis
Strain(s): 6, 87, 88, 89, 90, 91, 98, 99, 100, 101, 102, 104, 105, 106, 108, 109, 110, 111, 112, 298, 299, 604, 1055, 1061, 1068, 1076, 1090, 1091, 1105, 1122, 1142, 1164

Kondo, R., Kagiya, G., Hiroishi, S., Watanabe, M. 2000 Genetic typing of a bloom-forming cyanobacterial genus Microcystis in Japan using 16S rRNA gene sequence analysis. Plankton Biol. Ecol., 47, 1-6.
Keywords: genetic typing; 16S rDNA; Microcystis; cyanobacteria; phylogenetic analysis
Strain(s): 87, 88, 89, 90, 91, 98, 99, 100, 101, 102, 104, 105, 106, 108, 109, 110, 111, 112, 298, 299, 604, 1055, 1061, 1068, 1076, 1090, 1091, 1105, 1122, 1142, 1164

Ishida, K., Okita, Y., Matsuda, H., Okino, T., Murakami, M. 1999 Aeruginosins, protease inhibitors from the cyanobacterium Microcystis aeruginosa. Tetrahedron, 55, 10971-10988.
Strain(s): 89, 98, 298
DOI: 10.1016/S0040-4020(99)00621-3

Kondo, R., Kagiya, G., Hiroishi, S., Watanabe, M., Hata, Y. 1999 Rapid DNA extraction from a bloom-forming cyanobacterium. Microbes Environ., 14, 157-161.
Keywords: DNA extraction; PCR; Microcystis; cyanobacteria
Strain(s): 87, 88, 89, 90, 91, 98, 99, 100, 101, 102, 104, 105, 106, 108, 109, 110, 111, 112, 298, 299, 604, 1055, 1061, 1068, 1076, 1090, 1091, 1105, 1122, 1142, 1164
DOI: 10.1264/jsme2.14.157

Li, R., Yokota, A., Sugiyama, J., Watanabe, M., Hiroki, M., Watanabe, M. M. 1998 Chemotaxonomy of planktonic cyanobacteria based on non-polar and 3-hydroxy fatty acid composition. Phycol. Res., 46, 21-28.
Keywords: 3-hydroxy fatty acids; chemotaxonomy; fatty acid; non-polar fatty acids; planktonic cyanobacteria
Strain(s): 27, 40, 44, 76, 78, 79, 80, 81, 87, 89, 98, 102, 104, 111, 112, 204, 205, 207, 298, 604, 810, 821, 825, 826
DOI: 10.1111/j.1440-1835.1998.tb00093.x

Kondo, R., Kagiya, G., Yuki, Y., Hiroishi, S., Watanabe, M. 1998 Taxonomy of a bloom-forming cyanobacterial genus Microcystis. Nippon Suisan Gakkaishi, 64, 291-292 (in Japanese with English title).
Strain(s): 87, 88, 89, 90, 91, 98, 99, 100, 101, 102, 104, 105, 106, 107, 108, 109, 110, 111, 112, 298, 299, 604

Neilan, B. A., Jacobs, D., Goodman, A. E. 1995 Genetic diversity and phylogeny of toxic cyanobacteria determined by DNA polymorphisms within the phycocyanin locus. Appl. Environ. Microbiol., 61, 3875-3883.
Strain(s): 24, 40, 41, 44, 73, 76, 78, 80, 81, 87, 89, 98, 102, 107, 111, 112, 204, 207, 298
PubMed: 8526499

Neilan, B. A., Hawkins, P. R., Cox, P. T., Goodman, A. E. 1994 Towards a molecular taxonomy for the bloom-forming cyanobacteria. Aust. J. Mar. Freshwater Res., 45, 869-873.
Keywords: 16S rRNA; PCR; Microcystis; Anabaena; DNA sequence
Strain(s): 41, 78, 89, 102, 107, 111
DOI: 10.1071/MF9940869

Neilan, B. A., Cox, P. T., Hawkins, P. R., Goodman, A. E. 1994 16S ribosomal RNA gene sequence and phylogeny of toxic Microcystis sp. (Cyanobacteria). DNA Seq., 4, 333-337.
Keywords: Cyanobacteria; Microcystis; 165 rRNA gene; DNA sequence; taxonomy; phylogeny
Strain(s): 89
DOI: 10.3109/10425179409020861

Watanabe, M. M., Suda, S., Kasai, F., Sawaguchi, T. 1985 Axenic cultures of the three species of Microcystis (Cyanophyta = Cyanobacteria). Bull. JFCC, 1, 57-63.
Strain(s): 89, 102, 107, 111, 298

Watanabe, M. M., Kasai, F., Hiwatari, T., Suda, S., Nei, T. 1984 Cryopreservation of microalgae in liquid nitrogen - Survival of algal cultures after freezing. Jpn. J. Freez. Dry., 30, 23-26 (in Japanese with English title).
Strain(s): 18, 19, 21, 24, 26, 29, 31, 39, 40, 41, 44, 45, 46, 50, 73, 74, 75, 76, 77, 80, 81, 87, 88, 89, 90, 91, 93, 95, 98, 99, 100, 101, 120, 122, 131, 135, 137, 144, 151, 204, 207, 213, 257, 263, 379, 415, 2219, 2235, 2239, 2579

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