Strain number NIES-4474  
Phylum Cyanophyta  
Class Cyanophyceae  
Scientific name Coelosphaerium sp.  
Former name  
Common name Blue-green alga ; Cyanobacteria  
Locality (Date of collection) Lake Shinji, Shimane, Japan (2009-10-05)  
Latitude / Longitude 35.4504 / 132.9599 
Habitat (Isolation source) Brackish water (Lake water)  
History < Ohtani, Shuji < Godo, Toshiyuki  
Isolator (Date of isolation) Godo, Toshiyuki (2009-10-08)  
Identified by Ohtani, Shuji  
State of strain Cryopreservation; Unialgal; Clonal; Axenic[2022 Mar]  
Culture condition
(Preculture condition)
Medium:  CA  
Temperature:  20 C
Light intensity:  14-16 µmol photons/m2/sec, L/D cycle:  10L:14D
Duration:  1 M  
Gene information 16S-ITS-23S ( LC331270 ) , geoA ( LC331272 )  
Cell size (min - max) 2 - 3 μm  
Organization Colonial 
Characteristics Offensive odor ; geosmin production (Hayashi et al. 2019)  
Other strain no. Other strain no. : G3  
Remarks Cryopreserved; Axenic 
Hayashi, S., Ohtani, S., Godo, T., Nojiri, Y., Saki, Y., Esumi, T., Kamiya, H. 2019 Identification of geosmin biosynthetic gene in geosmin-producing colonial cyanobacteria Coelosphaerium sp. and isolation of geosmin non-producing Coelosphaerium sp. from brackish Lake Shinji in Japan. Harmful Algae, 84, 19-26.
Keywords: Geosmin; Geosmin synthase gene; Coelosphaerium sp.Geosmin-producing and non-producing strains; Lake Shinji
Strain(s): 447344744475 
PubMed: 31128804
DOI: 10.1016/j.hal.2019.01.010

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