Strain number NIES-4442  
Phylum Haptophyta  
Class Coccolithophyceae  
Scientific name Braarudosphaera bigelowii (Gran & Braarud) Deflandre  
Synonym Pontosphaera bigelowii Gran & Braarud 1935; Chrysochromulina parkeae J.C.Green & Leadbeater 1972  
Former name  
Common name  
Locality (Date of collection) Ikenoura Port, Ikenoura, Uranouchifukura, Susaki Kochi Japan (2017-05-24)  
Latitude / Longitude 33.4094 / 133.412 
Habitat (Isolation source) Marine (Seawater)  
History < Tsukakoshi, Chinatsu; Ishida, Ken-ichiro  
Isolator (Date of isolation) Tsukakoshi, Chinatsu (2017-**-**)  
Identified by Tsukakoshi, Chinatsu  
State of strain Subculture; Unialgal; Clonal; Non-axenic  
Culture condition
(Preculture condition)
Medium:  ESM  
Temperature:  20 C
Light intensity:  10-17 µmol photons/m2/sec, L/D cycle:  10L:14D
Duration:  45 D  
Gene information 16S rRNA ( LC595682 ) , 18S rRNA ( LC595681 )  
Cell size (min - max) 10 - 15 μm  
Organization Unicellular; Flagellate 
Characteristics Loss of endosymbionts  
Other strain no. Other strain no. : KC15-24  
Remarks Unstable; Fragile species to transportation stresses; Fragile species to temperature changes; Read and agree "How to order 4.1" 
Movie  
Reference
Suzuki, S., Kawachi, M., Tsukakoshi, C., Nakamura, A., Hagino, K., Inouye, I., Ishida, K. 2021 Unstable relationship between Braarudosphaera bigelowii (= Chrysochromulina parkeae) and its nitrogen-fixing endosymbiont. Front. Plant Sci., 12, 749895 (article ID).
Keywords: nitrogen fixation; endosymbiosis; haptophyte; UCYN-A; reductive evolution
Strain(s): 38654442 
DOI: 10.3389/fpls.2021.749895

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