Strain number NIES-4434  
Phylum Chlorophyta  
Class Chlorophyceae  
Scientific name Volvox longispiniferus Nozaki & Mahakham  
Synonym  
Former name  
Common name Green alga ; Volvocine alga  
Locality (Date of collection) Nong Ya Ma Reservoir, Yang Talat District, Kalasin Province, Thailand (2019-11-01)  
Latitude / Longitude 16.470708333 / 103.273763889 
Habitat (Isolation source) Freshwater (Reservoir water)  
History < Nozaki, Hisayoshi < Mahakham, Wuttipon  
Isolator (Date of isolation) Mahakham, Wuttipong (2019-11-01)  
Identified by Nozaki, Hisayoshi  
State of strain Subculture; Unialgal; Clonal; Axenic[2022 Mar]  
Culture condition
(Preculture condition)
Medium:  AF-6/3AF-6  
Temperature:  20 C
Light intensity:  22-25 µmol photons/m2/sec, L/D cycle:  10L:14D
Duration:  21 D  
Gene information 18S-ITS1-5.8S-ITS2-28S ( LC546057 ) , rbcL ( LC546062 ) , psbC ( LC546067 )  
Cell size (min - max)  
Organization Colonial; Flagellate 
Characteristics Phototaxis ; Homothallic ; Monoecious  
Other strain no. Other strain no. : 1101-NZ-5  
Remarks Fragile species to transportation stresses; Read and agree "How to order 4.1"; Axenic 
Movie  
Reference
Nozaki, H., Mahakham, W., Heman, W., Matsuzaki, R., Kawachi, M. 2020 A new preferentially outcrossing monoicous species of Volvox sect. Volvox (Chlorophyta) from Thailand. PLoS One, 15, e0235622 (article ID).
Strain(s): 273627403382338338744021433644324433443444354436 
PubMed: 32614898
DOI: 10.1371/journal.pone.0235622

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