A big image opens when the thumbnail is clicked.(nies-0296.jpg)
Strain number NIES-296  
Phylum Streptophyta  
Class Mesostigmatophyceae  
Scientific name Mesostigma viride Lauterborn  
Former name  
Common name  
Locality (Date of collection) Yoshino Park, Mitsukaido, Ibaraki, Japan (1985-07-03)  
Latitude / Longitude 36.076428 / 140.001716 
Habitat (Isolation source) Freshwater (Lake water)  
History < Suda, Shoichiro  
Isolator (Date of isolation) Suda, Shoichiro (1985-07-08)  
Identified by Inouye, Isao  
State of strain Subculture; Unialgal; Clonal; Axenic[2018 Jan]  
Culture condition
(Preculture condition)
Medium:  C  
Temperature:  20 C
Light intensity:  24-39 µmol photons/m2/sec, L/D cycle:  10L:14D
Duration:  25 D  
Gene information Whole-genome ( RPFO01000000 ) , Mitochondria DNA ( AF353999 ) , Plastid DNA ( AF166114 ) , Root cap protein 1-8 mRNAs ( DQ287936-DQ287943 ) , ftsZ ( AB673433 )  
Cell size (min - max) - 18 μm  
Organization Unicellular; Flagellate 
Characteristics Genome decoded strain (Lemieux, C. et al. 2000, Turmel, M. et al. 2002b, Liang et al. 2020)  
Other strain no. Other strain no. : KY-14  
Remarks Unstable; Fragile species to transportation stresses; Read and agree "How to order 4.1"; Axenic 
Movie https://www.youtube.com/watch?v=En3oZr0DE64&list=PLFTJlLsZcVbiJY3ygYAPKeVOfDdOh9UEW&index=11  
Davison, H. R., Pilgrim, J., Wybouw, N., Parker, J., Pirro, S., Hunter-Barnett, S., Campbell, P. M., Blow, F., Darby, A. C., Hurst, G. D. D., Siozios, S. 2022 Genomic diversity across the Rickettsia and ‘Candidatus Megaira’ genera and proposal of genus status for the Torix group. Nat. Commun., 13, 2630 (article ID).
Strain(s): 296425 
PubMed: 35551207
DOI: 10.1038/s41467-022-30385-6

Bock, N. A., Charvet, S., Burns, J., Gyaltshen, Y., Rozenberg, A., Duhamel ,S., Kim, E. 2021 Experimental identification and in silico prediction of bacterivory in green algae. ISME J., 15, 1987-2000.
Strain(s): 254296626995 
PubMed: 33649548
DOI: 10.1038/s41396-021-00899-w

Aso, M., Matsumae, R., Tanaka, A., Tanaka, R., Takabayashi, A. 2021 Unique peripheral antennas in the photosystems of the streptophyte alga Mesostigma viride. Plant Cell Physiol., 62, 436–446.
Keywords: Green algae; Iso-seq; Mesostigma viride; Native-PAGE; Photosystem
Strain(s): 296 
PubMed: 33416834
DOI: 10.1093/pcp/pcaa172

Liang, Z., Geng, Y., Ji, C., Du, H., Wong, C. E., Zhang, Q., Zhang, Y., Zhang, P., Riaz, A., Chachar, S., Ding, Y., Wen, J., Wu, Y., Wang, M., Zheng, H., Wu, Y., Demko, V., Shen, L., Han, X., Zhang, P., Gu, X., Yu, H. 2020 Mesostigma viride genome and transcriptome provide insights into the origin and evolution of Streptophyta. Adv. Sci., 7, 1901850 (article ID).
Keywords: Mesostigma viride; evolution; green algae; multicellularity; Streptophyta
Strain(s): 296 
PubMed: 31921561
DOI: 10.1002/advs.201901850

Yokono, M., Takabayashi, A., Kishimoto, J., Fujita, T., Iwai, M., Murakami, A., Akimoto, S., Tanaka, A. 2019 The PSI-PSII megacomplex in green plants. Plant Cell Physiol., 60, 1098–1108.
Keywords: Energy transfer; Nonphotochemical quenching; Photosystem
Strain(s): 14429630322852541 
PubMed: 30753722
DOI: 10.1093/pcp/pcz026

Yang, A., Narechania, A., Kim, E. 2016 Rickettsial endosymbiont in the “early‐diverging” streptophyte green alga Mesostigma viride. J. Phycol., 52, 219-229.
Keywords: endosymbiosis; green algae; Mesostigma ; Rickettsiales; symbiosis; transcriptome
Strain(s): 296475995 
PubMed: 27037587
DOI: 10.1111/jpy.12385

Hamana, K., Niitsu, M., Hayashi, H. 2013 Occurrence of homospermidine and thermospermine as a cellular polyamine in unicellular chlorophyte and multicellular charophyte green algae J. Gen. Appl. Micobiol., 59, 313-319.
Keywords: green alga; homospermidine; polyamine; thermospermine
Strain(s): 1815116025529630636036364072283614091410141114191837216421993374 
PubMed: 24005181
DOI: 10.2323/jgam.59.313

Hua, JM., Smith, D. R., Borza, T., Lee, R. W. 2012 Similar relative mutation rates in the three genetic compartments of Mesostigma and Chlamydomonas. Protist, 163, 105-115.
Keywords: Chlamydomonas; genome architecture; Mesostigma; mutation rate; silent substitution rate
Strain(s): 296476 
PubMed: 21621456
DOI: 10.1016/j.protis.2011.04.003

Miyagishima, S., Suzuki, K., Okazaki, K., Kabeya, Y. 2012 Expression of the nucleus-encoded chloroplast division genes and proteins regulated by the algal cell cycle. Mol. Biol. Evol., 29, 2957-2970.
Keywords: cell cycle; chloroplast division; endosymbiosis; endosymbiotic gene transfer
Strain(s): 296484547133221703377 
PubMed: 22490821
DOI: 10.1093/molbev/mss102

Hua, J., Lee, R.W. 2012 Factors affecting codon bias in the mitochondrial genomes of the Streptophyte Mesostigma viride and the Chlorophyte Chlamydomonas reinhardtii. J. Eucaryot. Microbiol., 59, 287-289.
Keywords: Green algae; mutation; selection; synonymous codon usage
Strain(s): 296 
PubMed: 22340021
DOI: 10.1111/j.1550-7408.2011.00613.x

Nedelcu, A. M., Borza, T., Lee, R. W. 2006 A land-plant specific multigene family in the unicellular Mesostigma argues for its close relationship to Streptophyta. Mol. Biol. Evol., 23, 1011-1015.
Keywords: Mesostigma viride; land plant evolution; Streptophyta; histogenetic meristem; gene family
Strain(s): 296 
PubMed: 16476689
DOI: 10.1093/molbev/msj108

Hamana, K., Aizaki T., Arai, E., Saito, A., Uchikata, K., Ohnishi, H. 2004 Distribution of norspermidine s a cellular polyamine within micro green algae including non-photosynthetic achlorophyllous Polytoma, Polytomella, Prototheca and Helicosporidium. J. Gen. Appl. Microbiol., 50, 289-295.
Keywords: Chlorophyta; green alga; Helicosporidium; norspermidine; polyamine; Polytoma; Polytomella; Prototheca
Strain(s): 18251254255296329483486536 

Laflamme, M., Lee, R. W. 2003 Mitochondrial genome conformation among CW-group Chlorophycean algae. J. Phycol., 39, 213-220.
Keywords: Chlamydomonas; Chlorophyceae; gel electrophoresis; mitochondrial DNA conformation; phylogeny
Strain(s): 296484 
DOI: 10.1046/j.1529-8817.2003.02045.x

Yoshii, Y., Takaichi, S., Maoka, T., Inouye, I. 2003 Photosynthetic pigment composition in the primitive green alga Mesostigma viride (Prasinophyceae): phylogenetic and evolutionary implications. J. Phycol., 39, 570-576.
Keywords: abscisic acid; carotenoid; Mesostigma; neoxanthin; siphonaxanthin; siphonaxanthin ester
Strain(s): 296475476477 
DOI: 10.1046/j.1529-8817.2003.02098.x

Turmel, M., Otis, C., Lemieux, C. 2002b The complete mitochondrial DNA sequence of Mesostigma viride identifies this green alga as the earliest green plant divergence and predicts a highly compact mitochondrial genome in the ancestor of all green plants. Mol. Biol. Evol., 19, 24-38.
Keywords: green aglae; Mesostigma viride;mitochondrial DNA; group I introns; group II introns; trans-splicing
Strain(s): 296 
PubMed: 11752187
DOI: 10.1093/oxfordjournals.molbev.a003979

Turmel, M., Ehara, M., Otis, C., Lemieux, C. 2002a Phylogenetic relationships among streptophytes as inferred from chloroplast small and large subunit rRNA gene sequences. J. Phycol., 38, 364-375.
Keywords: Charophyceae; chloroplast rRNA genes; introns; land plants; phylogeny; Streptophyta
Strain(s): 296484 
DOI: 10.1046/j.1529-8817.2002.01163.x

Schwender, J., Gemünden, C., Lichtenthaler, H. K. 2001 Chlorophyta exclusively use the 1-deoxyxylulose 5-phosphate/2-C-methylerythritol 4-phosphate pathway for the biosynthesis of isoprenoids. Planta, 212, 416-423.
Keywords: Charophyta; 1-Deoxy-D-xylulose; Green alga; Isopentenyl diphosphate; Isoprenoid biosynthesis; Mevalonate
Strain(s): 296 
PubMed: 11289606
DOI: 10.1007/s004250000409

Iwamoto, K. & Ikawa, T. 2000 A novel glycolate oxidase requiring flavin mononucleotide as the cofactor in the prasinophycean alga Mesostigma viride. Plant Cell Physiol., 41, 988-991.
Keywords: flavin mononucleotide (FMN)-requiring enzyme; glycolate metabolism; glycolate oxidase; glycolate-oxidizing enzyme; Mesostigma viride; Prasinophyceae
Strain(s): 296 
PubMed: 11038060
DOI: 10.1093/pcp/pcd020

Lemieux, C., Otis, C., Turmel, M. 2000 Ancestral chloroplast in Mesostigma viride reveals an early branch of green plant evolution. Nature, 403, 649-652.
Strain(s): 296 
PubMed: 10688199
DOI: 10.1038/35001059

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