Strain Data

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Strain number		NIES-296
Phylum		Streptophyta
Class		Mesostigmatophyceae
Scientific name		Mesostigma viride Lauterborn
Synonym
Former name
Common name
Locality (Date of collection)		Yoshino Park, Mitsukaido, Ibaraki, Japan (1985-07-03)
Latitude / Longitude		36.076428 / 140.001716
Habitat (Isolation source)		Freshwater (Lake water)
History		< Suda, Shoichiro
Isolator (Date of isolation)		Suda, Shoichiro (1985-07-08)
Identified by		Inouye, Isao
State of strain		Subculture; Unialgal; Clonal; Axenic[2018 Jan]
Culture condition (Preculture condition)		Medium: C Temperature: 20 C Light intensity: 24-39 µmol photons/m²/sec, L/D cycle: 10L:14D Duration: 25 D
Gene information		Whole-genome ( RPFO01000000 ) , Mitochondria DNA ( AF353999 ) , Plastid DNA ( AF166114 ) , Root cap protein 1-8 mRNAs ( DQ287936-DQ287943 ) , ftsZ ( AB673433 )
Cell size (min - max)		- 18 μm
Organization		Unicellular; Flagellate
Characteristics		Genome decoded strain (Lemieux, C. et al. 2000, Turmel, M. et al. 2002b, Liang et al. 2020)
Other strain no.		Other strain no. : KY-14
Remarks		Unstable; Fragile species to transportation stresses; Read and agree "How to order 4.1"; Axenic
Movie		https://www.youtube.com/watch?v=En3oZr0DE64&list=PLFTJlLsZcVbiJY3ygYAPKeVOfDdOh9UEW&index=11

Reference
Davison, H. R., Pilgrim, J., Wybouw, N., Parker, J., Pirro, S., Hunter-Barnett, S., Campbell, P. M., Blow, F., Darby, A. C., Hurst, G. D. D., Siozios, S. 2022 Genomic diversity across the Rickettsia and ‘Candidatus Megaira’ genera and proposal of genus status for the Torix group. Nat. Commun., 13, 2630 (article ID). Strain(s): 296, 425 PubMed: 35551207 DOI: 10.1038/s41467-022-30385-6 Bock, N. A., Charvet, S., Burns, J., Gyaltshen, Y., Rozenberg, A., Duhamel ,S., Kim, E. 2021 Experimental identification and in silico prediction of bacterivory in green algae. ISME J., 15, 1987-2000. Strain(s): 254, 296, 626, 995 PubMed: 33649548 DOI: 10.1038/s41396-021-00899-w Aso, M., Matsumae, R., Tanaka, A., Tanaka, R., Takabayashi, A. 2021 Unique peripheral antennas in the photosystems of the streptophyte alga Mesostigma viride. Plant Cell Physiol., 62, 436–446. Keywords: Green algae; Iso-seq; Mesostigma viride; Native-PAGE; Photosystem Strain(s): 296 PubMed: 33416834 DOI: 10.1093/pcp/pcaa172 Liang, Z., Geng, Y., Ji, C., Du, H., Wong, C. E., Zhang, Q., Zhang, Y., Zhang, P., Riaz, A., Chachar, S., Ding, Y., Wen, J., Wu, Y., Wang, M., Zheng, H., Wu, Y., Demko, V., Shen, L., Han, X., Zhang, P., Gu, X., Yu, H. 2020 Mesostigma viride genome and transcriptome provide insights into the origin and evolution of Streptophyta. Adv. Sci., 7, 1901850 (article ID). Keywords: Mesostigma viride; evolution; green algae; multicellularity; Streptophyta Strain(s): 296 PubMed: 31921561 DOI: 10.1002/advs.201901850 Yokono, M., Takabayashi, A., Kishimoto, J., Fujita, T., Iwai, M., Murakami, A., Akimoto, S., Tanaka, A. 2019 The PSI-PSII megacomplex in green plants. Plant Cell Physiol., 60, 1098–1108. Keywords: Energy transfer; Nonphotochemical quenching; Photosystem Strain(s): 144, 296, 303, 2285, 2541 PubMed: 30753722 DOI: 10.1093/pcp/pcz026 Yang, A., Narechania, A., Kim, E. 2016 Rickettsial endosymbiont in the “early‐diverging” streptophyte green alga Mesostigma viride. J. Phycol., 52, 219-229. Keywords: endosymbiosis; green algae; Mesostigma ; Rickettsiales; symbiosis; transcriptome Strain(s): 296, 475, 995 PubMed: 27037587 DOI: 10.1111/jpy.12385 Hamana, K., Niitsu, M., Hayashi, H. 2013 Occurrence of homospermidine and thermospermine as a cellular polyamine in unicellular chlorophyte and multicellular charophyte green algae J. Gen. Appl. Micobiol., 59, 313-319. Keywords: green alga; homospermidine; polyamine; thermospermine Strain(s): 18, 151, 160, 255, 296, 306, 360, 363, 640, 722, 836, 1409, 1410, 1411, 1419, 1837, 2164, 2199, 3374 PubMed: 24005181 DOI: 10.2323/jgam.59.313 Hua, JM., Smith, D. R., Borza, T., Lee, R. W. 2012 Similar relative mutation rates in the three genetic compartments of Mesostigma and Chlamydomonas. Protist, 163, 105-115. Keywords: Chlamydomonas; genome architecture; Mesostigma; mutation rate; silent substitution rate Strain(s): 296, 476 PubMed: 21621456 DOI: 10.1016/j.protis.2011.04.003 Miyagishima, S., Suzuki, K., Okazaki, K., Kabeya, Y. 2012 Expression of the nucleus-encoded chloroplast division genes and proteins regulated by the algal cell cycle. Mol. Biol. Evol., 29, 2957-2970. Keywords: cell cycle; chloroplast division; endosymbiosis; endosymbiotic gene transfer Strain(s): 296, 484, 547, 1332, 2170, 3377 PubMed: 22490821 DOI: 10.1093/molbev/mss102 Hua, J., Lee, R.W. 2012 Factors affecting codon bias in the mitochondrial genomes of the Streptophyte Mesostigma viride and the Chlorophyte Chlamydomonas reinhardtii. J. Eucaryot. Microbiol., 59, 287-289. Keywords: Green algae; mutation; selection; synonymous codon usage Strain(s): 296 PubMed: 22340021 DOI: 10.1111/j.1550-7408.2011.00613.x Nedelcu, A. M., Borza, T., Lee, R. W. 2006 A land-plant specific multigene family in the unicellular Mesostigma argues for its close relationship to Streptophyta. Mol. Biol. Evol., 23, 1011-1015. Keywords: Mesostigma viride; land plant evolution; Streptophyta; histogenetic meristem; gene family Strain(s): 296 PubMed: 16476689 DOI: 10.1093/molbev/msj108 Hamana, K., Aizaki T., Arai, E., Saito, A., Uchikata, K., Ohnishi, H. 2004 Distribution of norspermidine s a cellular polyamine within micro green algae including non-photosynthetic achlorophyllous Polytoma, Polytomella, Prototheca and Helicosporidium. J. Gen. Appl. Microbiol., 50, 289-295. Keywords: Chlorophyta; green alga; Helicosporidium; norspermidine; polyamine; Polytoma; Polytomella; Prototheca Strain(s): 18, 251, 254, 255, 296, 329, 483, 486, 536 Laflamme, M., Lee, R. W. 2003 Mitochondrial genome conformation among CW-group Chlorophycean algae. J. Phycol., 39, 213-220. Keywords: Chlamydomonas; Chlorophyceae; gel electrophoresis; mitochondrial DNA conformation; phylogeny Strain(s): 296, 484 DOI: 10.1046/j.1529-8817.2003.02045.x Yoshii, Y., Takaichi, S., Maoka, T., Inouye, I. 2003 Photosynthetic pigment composition in the primitive green alga Mesostigma viride (Prasinophyceae): phylogenetic and evolutionary implications. J. Phycol., 39, 570-576. Keywords: abscisic acid; carotenoid; Mesostigma; neoxanthin; siphonaxanthin; siphonaxanthin ester Strain(s): 296, 475, 476, 477 DOI: 10.1046/j.1529-8817.2003.02098.x Turmel, M., Otis, C., Lemieux, C. 2002b The complete mitochondrial DNA sequence of Mesostigma viride identifies this green alga as the earliest green plant divergence and predicts a highly compact mitochondrial genome in the ancestor of all green plants. Mol. Biol. Evol., 19, 24-38. Keywords: green aglae; Mesostigma viride;mitochondrial DNA; group I introns; group II introns; trans-splicing Strain(s): 296 PubMed: 11752187 DOI: 10.1093/oxfordjournals.molbev.a003979 Turmel, M., Ehara, M., Otis, C., Lemieux, C. 2002a Phylogenetic relationships among streptophytes as inferred from chloroplast small and large subunit rRNA gene sequences. J. Phycol., 38, 364-375. Keywords: Charophyceae; chloroplast rRNA genes; introns; land plants; phylogeny; Streptophyta Strain(s): 296, 484 DOI: 10.1046/j.1529-8817.2002.01163.x Schwender, J., Gemünden, C., Lichtenthaler, H. K. 2001 Chlorophyta exclusively use the 1-deoxyxylulose 5-phosphate/2-C-methylerythritol 4-phosphate pathway for the biosynthesis of isoprenoids. Planta, 212, 416-423. Keywords: Charophyta; 1-Deoxy-D-xylulose; Green alga; Isopentenyl diphosphate; Isoprenoid biosynthesis; Mevalonate Strain(s): 296 PubMed: 11289606 DOI: 10.1007/s004250000409 Iwamoto, K. & Ikawa, T. 2000 A novel glycolate oxidase requiring flavin mononucleotide as the cofactor in the prasinophycean alga Mesostigma viride. Plant Cell Physiol., 41, 988-991. Keywords: flavin mononucleotide (FMN)-requiring enzyme; glycolate metabolism; glycolate oxidase; glycolate-oxidizing enzyme; Mesostigma viride; Prasinophyceae Strain(s): 296 PubMed: 11038060 DOI: 10.1093/pcp/pcd020 Lemieux, C., Otis, C., Turmel, M. 2000 Ancestral chloroplast in Mesostigma viride reveals an early branch of green plant evolution. Nature, 403, 649-652. Strain(s): 296 PubMed: 10688199 DOI: 10.1038/35001059

Reference

Davison, H. R., Pilgrim, J., Wybouw, N., Parker, J., Pirro, S., Hunter-Barnett, S., Campbell, P. M., Blow, F., Darby, A. C., Hurst, G. D. D., Siozios, S. 2022 Genomic diversity across the Rickettsia and ‘Candidatus Megaira’ genera and proposal of genus status for the Torix group. Nat. Commun., 13, 2630 (article ID).
Strain(s): 296, 425
PubMed: 35551207
DOI: 10.1038/s41467-022-30385-6

Bock, N. A., Charvet, S., Burns, J., Gyaltshen, Y., Rozenberg, A., Duhamel ,S., Kim, E. 2021 Experimental identification and in silico prediction of bacterivory in green algae. ISME J., 15, 1987-2000.
Strain(s): 254, 296, 626, 995
PubMed: 33649548
DOI: 10.1038/s41396-021-00899-w

Aso, M., Matsumae, R., Tanaka, A., Tanaka, R., Takabayashi, A. 2021 Unique peripheral antennas in the photosystems of the streptophyte alga Mesostigma viride. Plant Cell Physiol., 62, 436–446.
Keywords: Green algae; Iso-seq; Mesostigma viride; Native-PAGE; Photosystem
Strain(s): 296
PubMed: 33416834
DOI: 10.1093/pcp/pcaa172

Liang, Z., Geng, Y., Ji, C., Du, H., Wong, C. E., Zhang, Q., Zhang, Y., Zhang, P., Riaz, A., Chachar, S., Ding, Y., Wen, J., Wu, Y., Wang, M., Zheng, H., Wu, Y., Demko, V., Shen, L., Han, X., Zhang, P., Gu, X., Yu, H. 2020 Mesostigma viride genome and transcriptome provide insights into the origin and evolution of Streptophyta. Adv. Sci., 7, 1901850 (article ID).
Keywords: Mesostigma viride; evolution; green algae; multicellularity; Streptophyta
Strain(s): 296
PubMed: 31921561
DOI: 10.1002/advs.201901850

Yokono, M., Takabayashi, A., Kishimoto, J., Fujita, T., Iwai, M., Murakami, A., Akimoto, S., Tanaka, A. 2019 The PSI-PSII megacomplex in green plants. Plant Cell Physiol., 60, 1098–1108.
Keywords: Energy transfer; Nonphotochemical quenching; Photosystem
Strain(s): 144, 296, 303, 2285, 2541
PubMed: 30753722
DOI: 10.1093/pcp/pcz026

Yang, A., Narechania, A., Kim, E. 2016 Rickettsial endosymbiont in the “early‐diverging” streptophyte green alga Mesostigma viride. J. Phycol., 52, 219-229.
Keywords: endosymbiosis; green algae; Mesostigma ; Rickettsiales; symbiosis; transcriptome
Strain(s): 296, 475, 995
PubMed: 27037587
DOI: 10.1111/jpy.12385

Hamana, K., Niitsu, M., Hayashi, H. 2013 Occurrence of homospermidine and thermospermine as a cellular polyamine in unicellular chlorophyte and multicellular charophyte green algae J. Gen. Appl. Micobiol., 59, 313-319.
Keywords: green alga; homospermidine; polyamine; thermospermine
Strain(s): 18, 151, 160, 255, 296, 306, 360, 363, 640, 722, 836, 1409, 1410, 1411, 1419, 1837, 2164, 2199, 3374
PubMed: 24005181
DOI: 10.2323/jgam.59.313

Hua, JM., Smith, D. R., Borza, T., Lee, R. W. 2012 Similar relative mutation rates in the three genetic compartments of Mesostigma and Chlamydomonas. Protist, 163, 105-115.
Keywords: Chlamydomonas; genome architecture; Mesostigma; mutation rate; silent substitution rate
Strain(s): 296, 476
PubMed: 21621456
DOI: 10.1016/j.protis.2011.04.003

Miyagishima, S., Suzuki, K., Okazaki, K., Kabeya, Y. 2012 Expression of the nucleus-encoded chloroplast division genes and proteins regulated by the algal cell cycle. Mol. Biol. Evol., 29, 2957-2970.
Keywords: cell cycle; chloroplast division; endosymbiosis; endosymbiotic gene transfer
Strain(s): 296, 484, 547, 1332, 2170, 3377
PubMed: 22490821
DOI: 10.1093/molbev/mss102

Hua, J., Lee, R.W. 2012 Factors affecting codon bias in the mitochondrial genomes of the Streptophyte Mesostigma viride and the Chlorophyte Chlamydomonas reinhardtii. J. Eucaryot. Microbiol., 59, 287-289.
Keywords: Green algae; mutation; selection; synonymous codon usage
Strain(s): 296
PubMed: 22340021
DOI: 10.1111/j.1550-7408.2011.00613.x

Nedelcu, A. M., Borza, T., Lee, R. W. 2006 A land-plant specific multigene family in the unicellular Mesostigma argues for its close relationship to Streptophyta. Mol. Biol. Evol., 23, 1011-1015.
Keywords: Mesostigma viride; land plant evolution; Streptophyta; histogenetic meristem; gene family
Strain(s): 296
PubMed: 16476689
DOI: 10.1093/molbev/msj108

Hamana, K., Aizaki T., Arai, E., Saito, A., Uchikata, K., Ohnishi, H. 2004 Distribution of norspermidine s a cellular polyamine within micro green algae including non-photosynthetic achlorophyllous Polytoma, Polytomella, Prototheca and Helicosporidium. J. Gen. Appl. Microbiol., 50, 289-295.
Keywords: Chlorophyta; green alga; Helicosporidium; norspermidine; polyamine; Polytoma; Polytomella; Prototheca
Strain(s): 18, 251, 254, 255, 296, 329, 483, 486, 536

Laflamme, M., Lee, R. W. 2003 Mitochondrial genome conformation among CW-group Chlorophycean algae. J. Phycol., 39, 213-220.
Keywords: Chlamydomonas; Chlorophyceae; gel electrophoresis; mitochondrial DNA conformation; phylogeny
Strain(s): 296, 484
DOI: 10.1046/j.1529-8817.2003.02045.x

Yoshii, Y., Takaichi, S., Maoka, T., Inouye, I. 2003 Photosynthetic pigment composition in the primitive green alga Mesostigma viride (Prasinophyceae): phylogenetic and evolutionary implications. J. Phycol., 39, 570-576.
Keywords: abscisic acid; carotenoid; Mesostigma; neoxanthin; siphonaxanthin; siphonaxanthin ester
Strain(s): 296, 475, 476, 477
DOI: 10.1046/j.1529-8817.2003.02098.x

Turmel, M., Otis, C., Lemieux, C. 2002b The complete mitochondrial DNA sequence of Mesostigma viride identifies this green alga as the earliest green plant divergence and predicts a highly compact mitochondrial genome in the ancestor of all green plants. Mol. Biol. Evol., 19, 24-38.
Keywords: green aglae; Mesostigma viride;mitochondrial DNA; group I introns; group II introns; trans-splicing
Strain(s): 296
PubMed: 11752187
DOI: 10.1093/oxfordjournals.molbev.a003979

Turmel, M., Ehara, M., Otis, C., Lemieux, C. 2002a Phylogenetic relationships among streptophytes as inferred from chloroplast small and large subunit rRNA gene sequences. J. Phycol., 38, 364-375.
Keywords: Charophyceae; chloroplast rRNA genes; introns; land plants; phylogeny; Streptophyta
Strain(s): 296, 484
DOI: 10.1046/j.1529-8817.2002.01163.x

Schwender, J., Gemünden, C., Lichtenthaler, H. K. 2001 Chlorophyta exclusively use the 1-deoxyxylulose 5-phosphate/2-C-methylerythritol 4-phosphate pathway for the biosynthesis of isoprenoids. Planta, 212, 416-423.
Keywords: Charophyta; 1-Deoxy-D-xylulose; Green alga; Isopentenyl diphosphate; Isoprenoid biosynthesis; Mevalonate
Strain(s): 296
PubMed: 11289606
DOI: 10.1007/s004250000409

Iwamoto, K. & Ikawa, T. 2000 A novel glycolate oxidase requiring flavin mononucleotide as the cofactor in the prasinophycean alga Mesostigma viride. Plant Cell Physiol., 41, 988-991.
Keywords: flavin mononucleotide (FMN)-requiring enzyme; glycolate metabolism; glycolate oxidase; glycolate-oxidizing enzyme; Mesostigma viride; Prasinophyceae
Strain(s): 296
PubMed: 11038060
DOI: 10.1093/pcp/pcd020

Lemieux, C., Otis, C., Turmel, M. 2000 Ancestral chloroplast in Mesostigma viride reveals an early branch of green plant evolution. Nature, 403, 649-652.
Strain(s): 296
PubMed: 10688199
DOI: 10.1038/35001059

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