Strain number NIES-4342  
Phylum Chlorophyta  
Class Chlorophyceae  
Scientific name Volvox rousseletii G.S.West  
Former name  
Common name Green alga ; Volvocine alga  
Locality (Date of collection) Tanigahara water purification plant, Midori-ku, Sagamihara, Kanagawa, Japan (2018-09-20)  
Latitude / Longitude 35.59098 / 139.29636 
Habitat (Isolation source) Freshwater (Water)  
History < Kinbara, Ryosuke; Nozaki, Hisayoshi  
Isolator (Date of isolation) Kinbara, Ryosuke (2018-09-20)  
Identified by Kinbara, Ryosuke; Nozaki, Hisayoshi  
State of strain Subculture; Unialgal; Clonal; Non-axenic  
Culture condition
(Preculture condition)
Medium:  AF-6/3AF-6  
Temperature:  25 C
Light intensity:  22-25 µmol photons/m2/sec, L/D cycle:  10L:14D
Duration:  1 M  
Gene information 18S-ITS1-5.8S-ITS2-28S ( LC493805 )  
Cell size (min - max)  
Organization Colonial; Flagellate 
Characteristics Phototaxis ; Dioecious ; Heterothallic ; Male  
Other strain no. Other strain no. : v-tani-9  
Remarks Fragile species to transportation stresses; Read and agree "How to order 4.1" 
Kimbara, R., Isaka, N., Matsuzaki, R., Kawai-Toyooka, H., Kawachi, M., Nozaki, H. 2019 Morphological and molecular identification of the dioecious “African species Volvox rousseletii (Chlorophyceae) in the water column of a Japanese lake based on field-collected and cultured materials. PLoS One, 14, e0221632 (article ID).
Strain(s): 4336433743384339434043414342 
PubMed: 31465476
DOI: 10.1371/journal.pone.0221632

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