Strain number NIES-4336  
Phylum Chlorophyta  
Class Chlorophyceae  
Scientific name Volvox rousseletii G.S.West  
Synonym  
Former name  
Common name Green alga ; Volvocine alga  
Locality (Date of collection) Lake Sagami, Kanagawa, Japan (2015-06-10)  
Latitude / Longitude 35.61041 / 139.18583 
Habitat (Isolation source) Freshwater (Reservoir water)  
History < Nozaki, Hisayoshi  
Isolator (Date of isolation) Nozaki, Hisayoshi (2015-05-10)  
Identified by Kinbara, Ryosuke; Nozaki, Hisayoshi  
State of strain Subculture; Unialgal; Clonal; Non-axenic[2024 Jul]  
Culture condition
(Preculture condition)
Medium:  AF-6/3AF-6  
Temperature:  20 C
Light intensity:  24-26 µmol photons/m2/sec, L/D cycle:  10L:14D
Duration:  1 M  
Gene information 18S-ITS1-5.8S-ITS2-28S ( LC493797 ) , rbcL ( LC493808 ) , psbC ( LC493810 ) , act1 ( LC817968 )  
Cell size (min - max)  
Organization Colonial; Flagellate 
Characteristics Phototaxis ; Dioecious ; Heterothallic ; Male  
Other strain no. Other strain no. : 2015-0610-3v6  
Remarks Fragile species to transportation stresses; Read and agree "How to order 4.1" 
Movie  
Reference
Yamagishi, S., Yamamoto, K., Takahashi, K., Kawai-Toyooka, H., Suzuki, S., Matsuzaki, R., Yamaguchi, H., Kawachi, M., Higashiyama, T., Nozaki, H. 2024 Evolutionary analysis of MID homologs during the transition from homothallic species to heterothallic species in Volvox sect. Volvox (Chlorophyceae). Phycol. Res., 72, 46-55.
Keywords: male gamete; mating system; minus dominance gene (MID); molecular evolution; sex; sperm packet; volvocine green algae
Strain(s): 28945646054154454556957257458265265365473285185285787289189213621363170817101859245725692735273627403382338337803782378638743984398639884021402543364432453545364537 
DOI: 10.1111/pre.12538

Nozaki, H., Matsuzaki, R., Shimotori, K., Ueki, N., Heman, W., Mahakham, W., Yamaguchi, H., Tanabe, Y., Kawachi, M. 2024 Two species of the green algae Volvox sect. Volvox from the Japanese ancient lake, Lake Biwa. PLoS One, 19, e0310549 (article ID).
Strain(s): 543273627403874402140224258425943364337443446614662 
PubMed: 39312548
DOI: 10.1371/journal.pone.0310549

Nozaki, H., Mahakham, W., Heman, W., Matsuzaki, R., Kawachi, M. 2020 A new preferentially outcrossing monoicous species of Volvox sect. Volvox (Chlorophyta) from Thailand. PLoS One, 15, e0235622 (article ID).
Strain(s): 273627403382338338744021433644324433443444354436 
PubMed: 32614898
DOI: 10.1371/journal.pone.0235622

Kimbara, R., Isaka, N., Matsuzaki, R., Kawai-Toyooka, H., Kawachi, M., Nozaki, H. 2019 Morphological and molecular identification of the dioecious “African species Volvox rousseletii (Chlorophyceae) in the water column of a Japanese lake based on field-collected and cultured materials. PLoS One, 14, e0221632 (article ID).
Strain(s): 4336433743384339434043414342 
PubMed: 31465476
DOI: 10.1371/journal.pone.0221632

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