Strain number NIES-4333  
Phylum Haptophyta  
Class Prymnesiophyceae  
Scientific name Isochrysis sp.  
Synonym  
Former name  
Common name  
Locality (Date of collection) Lake Deadmoose, Saskatchewan, Canada (2014-09-**)  
Latitude / Longitude 52.297999 /  
Habitat (Isolation source) Salt water (Sediment)  
History < Shiraiwa, Yoshihiro < Araie, Hiroya; Atsumi, Mika  
Isolator (Date of isolation) Araie, Hiroya; Atsumi, Mika (2015-06-18)  
Identified by Araie, Hiroya  
State of strain Cryopreservation; Unialgal; Non-clonal; Non-axenic  
Culture condition
(Preculture condition)
Medium:  IMK  
Temperature:  20 C
Light intensity:  11-17 µmol photons/m2/sec, L/D cycle:  10L:14D
Duration:  45 D  
Gene information 28S rRNA ( LC322324 ) , 18S rRNA ( LC322317 )  
Cell size (min - max) 5 - 20 μm  
Organization Unicellular; Flagellate 
Characteristics alkenone production (Araie et al. 2018)  
Other strain no. Other strain no. : Dm3  
Remarks Cryopreserved 
Movie  
Reference
Araie, H., Nakamura, H., Toney, J. L., Haigg, H. A., Plancq, J., Shiratori, T., Leavitt, P. R., Seki, O., Ishida, K-I., Sawada, K., Suzuki, I., Shiraiwa, Y. 2018 Novel alkenone-producing strains of genus Isochrysis (Haptophyta) isolated from Canadian saline lakes show temperature sensitivity of alkenones and alkenoates. Org. Geochem., 121, 89-103.
Keywords: Alkenones; Alkenone unsaturation index; Canadian salt lakes; Chemotaxonomy; Haptophytes; Isochrysis; Long-chain alkyl ketones; Paleothermometer; UK37
Strain(s): 43314332433343344335 
DOI: 10.1016/j.orggeochem.2018.04.008

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