Strain number NIES-469  
Phylum Chlorophyta  
Class Chlorophyceae  
Scientific name Gonium pectorale var. pectorale O.F.Müller  
Synonym  
Former name  
Common name Green alga  
Locality (Date of collection) Kohoku-ku, Yokohama, Kanagawa, Japan (1979-04-06)  
Latitude / Longitude 35.528007 / 139.622842 
Habitat (Isolation source) Freshwater (Pond water)  
History < Nozaki, Hisayoshi  
Isolator (Date of isolation) Nozaki, Hisayoshi (1979-04-06)  
Identified by Nozaki, Hisayoshi  
State of strain Cryopreservation; Unialgal; Clonal; Axenic[2017 Dec]  
Culture condition
(Preculture condition)
Medium:  VT  
Temperature:  20 C
Light intensity:  8-11 µmol photons/m2/sec, L/D cycle:  10L:14D
Duration:  1 M  
Gene information 18S rRNA ( LC535334 )  
Cell size (min - max) - 15 μm  
Organization Colonial; Flagellate 
Characteristics Phototaxis ; Heterothallic ; Mating type (+) ; Crosses with NIES-468  
Other strain no. Other strain no. : 9406-12  
Remarks Cryopreserved; Axenic 
Movie  
Reference
Nozaki, H., Mori, F., Tanaka, Y., Matsuzaki, R., Yamaguchi, H., Kawachi, M. 2022 Cryopreservation of vegetative cells and zygotes of the multicellular volvocine green alga Gonium pectorale. BMC Microbiol., 22, 103 (article ID).
Strain(s): 2882904684695695706456466526536546557378518528852261226341214499450045014502 
PubMed: 35421922
DOI: 10.1186/s12866-022-02519-9

Mori, F., Erata, M., Watanabe, M. M. 2002 Cryopreservation of cyanobacteria and green algae in the NIES-Collection. Microbiol. Cult. Coll., 18, 45-55.
Keywords: cryopreservation; cyanobacteria; green algae; FDA staining method; NIES-Collection
Strain(s): 192122232425262728293031323334353637383940414445465051525354555658596061626364656667686970737475767778798081878990919293949596979899100101102103104105106107108109110111112119120122123125127128129130131132133134135137138139144147151152153154155156157158159160162163164165166167168170171172173174175176177178179180181182183185186187188189191192193194197198199200201202203204205206207208209210211212213214215216217224226227228229230231232241242243244245246248257259261263266267268287288289290294295297298299300301302303305306308309310312313329334337338339340341342349351359360361362375378379380382384385390394396397398415416418419421422423424425426427428429430431432433434436437438439440446447448449450451452453454455456457458459460464468469474478479480481503504505506507509510512514515522523524527528529530531532536537538539540541542543544545546564565566567568569570571572573574575576577578579580581582583584585592593594595596597598604610628630631632633634635636637638639640641642643644645646647648649650651652653654655656657658659660661662663664665666667672685686687688689690691692693694717718719720721722723724725726727728729 

Nozaki, H. 1984 Newly found facets in the asexual and sexual reproduction of Gonium pectorale (Chlorophyta, Volvocales). Jpn. J. Phycol. (Sôrui), 32, 130-133.
Keywords: asexual reproduction; Chlorophyta; flagellar emission; Gonium pectorale; isogamous conjugation; mating papilla; sexual reproduction; Volvocales
Strain(s): 468469 

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