Strain number NIES-4319  
Phylum Streptophyta  
Class Charophyceae  
Scientific name Closterium peracerosum-strigosum-littorale complex  
Former name  
Common name Desmid  
Locality (Date of collection) Asahata retarding basin, Ushida, Aoi-ku, Shizuoka, Shizuoka, Japan (2013-07-20)  
Latitude / Longitude 35.015750 / 138.402806 
Habitat (Isolation source) Freshwater (Pond water)  
History < Tsuchikane, Yuki  
Isolator (Date of isolation) Tsuchikane, Yuki (2013-07-20)  
Identified by Tsuchikane, Yuki  
State of strain Subculture; Unialgal; Clonal; Axenic[2022 Mar]  
Culture condition
(Preculture condition)
Medium:  CA  
Temperature:  20 C
Light intensity:  17 µmol photons/m2/sec, L/D cycle:  10L:14D
Duration:  1 M  
Gene information 18S rRNA ( LC361439 )  
Cell size (min - max) 130 - 135 μm  
Organization Unicellular 
Characteristics Heterothallic ; Isogamou ; Mating type (−) ; Mating group G (Tsuchikane et al. 2018)  
Other strain no. Other strain no. : Asa13-14  
Remarks Axenic 
Tsuchikane, Y., Kobayashi, H., Kato, M., Watanabe, J., Wu, J. T., Sekimoto, H. 2018 Identification of a new mating group and reproductive isolation in the Closterium peracerosum–strigosum–littorale complex. J. Plant Res., 131, 735–746.
Keywords: Alga; Closterium; Mating group; Reproductive isolation; Sex pheromone; Speciation
Strain(s): 535455646566676826126243194320 
PubMed: 29948484
DOI: 10.1007/s10265-018-1043-8

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