A big image opens when the thumbnail is clicked.(nies-0324.jpg)
Strain number NIES-324  
Phylum Heterokontophyta  
Class Bacillariophyceae  
Scientific name Skeletonema marinoi-dohrnii complex  
Former name Skeletonema costatum (Greville) Cleve  
Common name Centric diatom  
Locality (Date of collection) Osaka Bay Off Kobe, Hyogo, Japan (1985-07-02)  
Latitude / Longitude  
Habitat (Isolation source) Marine (Seawater)  
History < Osaka Pref. Fish. Exp. St.  
Isolator (Date of isolation) Yamochi, Susumu (1985-07-10)  
Identified by Yamochi, Susumu  
State of strain Subculture; Unialgal; Clonal; Axenic[2018 Feb]  
Culture condition
(Preculture condition)
Medium:  f/2  
Temperature:  15 C
Light intensity:  8 µmol photons/m2/sec, L/D cycle:  10L:14D
Duration:  1 M  
Gene information 18S rRNA ( AB488611 )  
Cell size (min - max) 3 - 10 μm  
Organization Unicellular 
Characteristics Red tide ; Test strain (Khanam et al. 2017) ; Planktonic ; DNA sequencing indicates this strain is included in Skeletonema marinoi/dohrnii clade  
Other strain no. Other strain no. : Sk-85su  
Remarks Axenic 
Onduka, T., Kono, K., Ito, M., Ohkubo, N., Hano, T., Ito, K., Mochida, K. 2020 Ecological risk assessment of an antifouling biocide triphenyl (octadecylamine) boron in the Seto Inland Sea, Japan. Mar. Pollut. Bull., 157, 111320 (article ID).
Keywords: Algal growth inhibition test; Crustacean reproduction test; Early-life stage toxicity test; Species sensitivity distribution
Strain(s): 324 
PubMed: 32658685
DOI: 10.1016/j.marpolbul.2020.111320

Hikihara, R., Yamasaki, Y., Shikata, T., Nakayama, N., Sakamoto, S., Kato, S., Hatate, H., Tanaka, R. 2020 Analysis of phytosterol, fatty acid, and carotenoid composition of 19 microalgae and 6 bivalve species. J. Aquat. Food Prod. Technol., 29, 461-479.
Keywords: Microalgae; bivalve; phytosterol; fucosterol; fatty acid
Strain(s): 110324 
DOI: 10.1080/10498850.2020.1749744

Hamamoto, Y., Honda, D. 2019 Nutritional intake of Aplanochytrium (Labyrinthulea, Stramenopiles) from living diatoms revealed by culture experiments suggesting the new prey–predator interactions in the grazing food web of the marine ecosystem. PLoS One, 14, e0208941 (article ID).
Strain(s): 3243712 
PubMed: 30625142
DOI: 10.1371/journal.pone.0208941

Kashiyama, Y. et al. 2019 Taming chlorophylls by early eukaryotes underpinned algal interactions and the diversification of the eukaryotes on the oxygenated Earth. ISME J., 13, 1899-1910.
Strain(s): 8254274286324331353381494623624997101210131015132013301333133413351372137713881390139613971408143814391441186819642008214221442149230023052325241124332498250225662584258625892590267728783374360437753876 
PubMed: 30809012
DOI: 10.1038/s41396-019-0377-0

Khanam, MR., Shimasaki, Y., Hosain, MZ., Mukai, K., Tsuyama, M., Qiu, X., Tasmin, R., Goto, H., Oshima, Y. 2017 Diuron causes sinking retardation and physiochemical alteration in marine diatoms Thalassiosira pseudonana and Skeletonema marinoi-dohrnii complex. Chemosphere, 175, 200-209.
Keywords: Sinking rate; Thalassiosira pseudonana; Skeletonema marinoi-dohrnii complex; Diuron
Strain(s): 324 
PubMed: 28222374
DOI: 10.1016/j.chemosphere.2017.02.054

Kohira, M., Matsumoto, T., Yamada, Y., Nanba, N., Kado, R. 2017 Effects of hydrogen peroxide on phytoplanktons, seaweed gametophytes, marine harpacticoid copepod and striped barnacle larvae. Sessile Organisms, 34, 1-12 (in Japanese with English summary).
Keywords: hydrogen peroxide; marine ecotoxicity; seaweed gametophytes; phytoplankton; zooplankton
Strain(s): 23532458613751429 
DOI: 10.4282/sosj.34.1

Mitani, E., Nakayama, F., Matsuwaki, I., Ichi, I., Kawabata, A., Kawachi, M., Kato, M. 2017 Fatty acid composition profiles of 235 strains of three microalgal divisions within the NIES Microbial Culture Collection. Microb. Resour. Syst., 33, 19-29.
Keywords: Cryptophyta; docosahexaenoic acid; eicosapentaenoic acid; fatty acid; Haptophyta; Heterokontophyta; microalgae
Strain(s): 189141517711152232252332342652742752762772782792802812822842933233243303333443453463473483503533723773883913954074084094134144174614624664875345485535565575585595605625875885895906036056226236956966976986997007017027037047057067077087097107117127137147157167417657667678058379971001100210031004100510061007100910111016101710441045104610471302130313241327133013391340134913531370137513761379138313841385138613871391139213931395139813991400140116991700173018131815181618261827183118621863186418651874196319641965197419751976214221432144214521472148230023312332235123632364236523692370237625062533253425352536253725902633266826892690269126932694269626972707271627172718272027222723272527262729273027312732277027712772277328392840284128422843284428592872287828903391 

Fuchida, S.,Yokoyama, A., Fukuchi, R., Ishibashi, J., Kawagucci, S., Kawachi, M., Koshikawa, H. 2017 Leaching of metals and metalloids from hydrothermal ore particulates and their effects on marine phytoplankton. ACS Omega, 2, 3175–3182.
Keywords: Geological materials; Microbiology; Separation science; Toxicology; Water pollution
Strain(s): 324 
PubMed: 30023687
DOI: 10.1021/acsomega.7b00081

Shimasaki, Y., Tsuyama, M., Tasmin, R., Khanam, M. R. M., Qiu, X., Oshima, Y. 2015 Comparative study of toxicological responses of chlorophyllrelated parameters in the marine diatom Skeletonema sp. exposed to tributyltin and Irgarol. Jpn. J. Environ. Toxicol., 18, 1-10.
Keywords: tributyltin; Irgarol; diatom; chlorophyll; photosynthesis
Strain(s): 324 
DOI: 10.11403/jset.18.1

Naito, K., Sakai, S., Fujinaga, S., Yoshinaga, I., Tanaka, M. 2014 Evolution of water quality at a tidal flat in the Ariake Sea by Algal Growth Potential test. J. life. Environ. Sci., 6, 17-28 (in Japanese with English summary).
Keywords: Ariake Sea; AGP test; Nutrient; Iron; Microalgae
Strain(s): 1324 

Qiu, X., Shimasaki, Y., Yoshida, Y., Matsubara, T., Yamasaki, Y., Kawaguchi, M., Honda, M., Mouri, K., Nakajima, Y., Tasmin, R., Kuno, K., Kawamura, Y., Honjo, T., Oshima, Y. 2014 Allelopathic effects of Skeletonema spp. may influence interspecific competition and bloom formation of co–occurring harmful flagellates. J. Fac. Agr., Kyushu Univ., 59, 373-382.
Strain(s): 110324 

Enomoto, J., Kimura, T., Nomura, Y., Kabayama, J., Maita, M. 2013 The safety of febantel for tiger puffer and its environmental impact. Nippon Suisan Gakkaishi, 79, 400-409 (in Japanese with English summary).
Keywords: tiger puffer; febantel; Heterobothrium okamotoi
Strain(s): 324 
DOI: 10.2331/suisan.79.400

Onduka, T., Ojima, D., Ito, M., Ito, K., Mochida, K., Fujii, K. 2013 Toxicity of degradation products of the antifouling biocide pyridine triphenylborane to marine organisms. Arch. Environ. Contam. Toxicol., 65, 724-732.
Strain(s): 324 
DOI: 10.1007/s00244-013-9945-x

Onduka,T., Ojima, D., Ito, M., Ito, K., Mochida, K., Fujii, K. 2013 Toxicity of the antifouling biocide Sea-Nine 211 to marine algae, crustacea, and a polychaete. Fish. Sci., 79, 999-1006.
Keywords: Risk assessment; Ecological toxicity; Coastal area of Japan; Perinereis nuntia; Sediment toxicity test; No-observed-effect concentration
Strain(s): 324 
DOI: 10.1007/s12562-013-0678-6

Qiu, XC., Yamasaki, Y., Shimasaki, Y., Gunjikake, H., Honda, M., Kawagushi, M., Matsubara, T., Nagasoe, S., Etoh, T., Matsui, S., Honjo, T., Oshima, Y. 2012 Allelopathy of the raphidophyte Heterosigma akashiwo against the dinoflagellate Akashiwo sanguinea is mediated via allelochemicals and cell contact. Mar. Ecol. Prog. Ser., 446, 107-118.
Keywords: Heterosigma akashiwo; Akashiwo sanguinea; Allelopathy; Polysaccharide−protein complex; Cell contact; Growth interaction; Growth simulation
Strain(s): 10324 
DOI: 10.3354/meps09476

Mochida, K., Onduka, T., Amano, H., Ito, M., Tanaka, H., Fujii, K. 2012 Use of species sensitivity distributions to predict no-effect concentrations of an antifouling biocide, pyridine triphenylborane, for marine organisms. Mar. Pollut. Bull., 64, 2807-2814.
Keywords: Algal growth inhibition test; Bayesian inference; Crustacean immobilization test; Fish acute toxicity; Primary risk analysis
Strain(s): 324 
PubMed: 23044030
DOI: 10.1016/j.marpolbul.2012.09.007

Onduka, T., Kakuno, A., Kono, K., Ito, K., Mochida, K., Fujii, K. 2012 Toxicity of chlorothalonil to marine organisms. Fish. Sci., 78, 1301-1308.
Keywords: Antifouling biocides; Toxicity; Early-life-stage Toxicity test; Predicted no-effect concentration
Strain(s): 324 
DOI: 10.1007/s12562-012-0562-9

Onduka, T., Ojima, D., Kakuno, A., Mochida, K., Ito, K., Koyama, J., Fujii, K. 2012 Nitrated polycyclic aromatic hydrocarbons in the marine environment: acute toxicities for organisms at three trophic levels. Jpn. J. Environ. Toxicol., 15, 1-10.
Keywords: alga; crustacean, fish, nitro-PAHs; predicted no effect concentrations
Strain(s): 324 
DOI: 10.11403/jset.15.1

Yamasaki, Y., Ohmichi, Y., Hirose, M., Shikata, T., Shimasaki, Y., Oshima, Y., Honjo, T. 2012 Low molecular weight allelochemicals produced by the diatom, Skeletonema costatum. Thalassas, 28, 9-17.
Keywords: Allelochemicals; Diatom; Growth inhibition; Heterosigma akashiwo; Raphidophyt; Skeletonema costatum
Strain(s): 10324 

Onduka, T., Mochida, K., Harino, H., Ito, K., Kakuno, A., Fujii, K. 2010 Toxicity of metal pyrithione photodegradation products to marine organisms with indirect evidence for their presence in seawater. Arch. Environ. Contam. Toxicol., 58, 991-997.
Strain(s): 324 
PubMed: 19967345
DOI: 10.1007/s00244-009-9430-8

Yamasaki, Y., Nagasoe, S., Tameishi, M., Shikata, T., Zou, Y., Jiang, Z., Matsubara, T., Shimasaki, Y., Yamaguchi, K., Oshima, Y., Honjo, T. 2010 The role of interactions between Prorocentrum minimum and Heterosigma akashiwo in bloom formation. Hydrobiologia, 641, 33-44.
Keywords: Allelopathy; growth and interaction; Heterosigma akashiwo; Prorocentrum minimum; Reactive oxygen species; Skeletonema costatum
Strain(s): 110324 
DOI: 10.1007/s10750-009-0052-y

Yamasaki, Y., Ohmichi, Y., Shikata, T., Hirose, M., Shimasaki, Y., Oshima, Y., Honjo, T. 2010 Species-specific allelopathic effects of the diatom Skeletonema costatum. Thalassas, 27, 21-32.
Keywords: Allelopathy; growth inhibition; growth promotion; Heterosigma akashiwo; low molecular weight allelochemicals; Skeletonema costatum; species-specific effect
Strain(s): 10324 

Yamasaki, Y., Shikata, T., Nukata, A., Ichiki, S., Nagasoe, S., Matsubara, T., Shimasaki, Y., Nakao, M., Yamaguchi, K., Oshima, Y., Oda, T., Ito, M., Jenkinson, I. R., Asakawa, M., Honjo, T. 2009 Extracellular polysaccharide-protein complexes of a harmful alga mediate the allelopathic control it exerts within the phytoplankton community. ISME J., 3, 808-817.
Keywords: allelopathy; growth inhibition; growth stimulation; harmful algal blooms (HABs); Heterosigma akashiwo; Skeletonema costatum
Strain(s): 10324 
PubMed: 19322242
DOI: 10.1038/ismej.2009.24

Kooistra, W. H. C. F., Sarno, D., Balzano, S., Gu, H., Andersen, R. A., Zingone, A. 2008 Global diversity and biogeography of Skeletonema species (Bacillariophyta). Protist, 159, 177-193.
Keywords: biogeography; cryptic species; distribution; LSU rDNA; phytoplankton; Skeletonema costatum
Strain(s): 1617223323324 
PubMed: 18042429
DOI: 10.1016/j.protis.2007.09.004

Oh, S. J., Kim, D.-I., Sajima, T., Shimasaki, Y., Matsuyama, Y., Oshima, Y., Honjo, T., Yang, H.-S. 2008 Effects of irradiance of various wavelengths from light-emitting diodes on the growth of the harmful dinoflagellate Heterocapsa circularisquama and the diatom Skeletonema costatum. Fish. Sci., 74, 137-145.
Keywords: Heterocapsa circularisquama; irradiance; light wavelengths; light-emitting diode; Skeletonema costatum
Strain(s): 324 
DOI: 10.1111/j.1444-2906.2007.01503.x

Matsubara, T., Nagasoe, S., Yamasaki, Y., Shikata, T., Shimasaki, Y., Oshima, Y., Honjo, T. 2008 Inhibitory effects of centric diatoms on the growth of the dinoflagellate Akashiwo sanguinea. Nippon Suisan Gakkaishi, 74, 598-606 (in Japanese with English summary).
Keywords: Akashiwo sanguinea; allelopathy; growth inhibition; centric diatoms
Strain(s): 324 
DOI: 10.2331/suisan.74.598

Fukao, T., Nishijima, T., Yamaguchi, H., Adachi, M. 2007 Utilization of urea by the 6 species of red tide phytoplankton. Bull. Plankton Soc. Japan, 54, 1-8 (in Japanese with English summary).
Keywords: growth; red tide; phytoplankton; urea; uptake kinetics
Strain(s): 16237323324605 

Yamasaki, Y., Nagasoe, S., Matsubara, T., Shikata, T., Shimasaki, Y., Oshima, Y., Honjo, T. 2007 Allelopathic interactions between the bacillariophyte Skeletonema costatum and the raphidophyte Heterosigma akashiwo. Mar. Ecol. Prog. Ser., 339, 83-92.
Keywords: Allelopathy; Bacillariophyceae; Skeletonema costatum; Raphidophyceae; Heterosigma akashiwo; Bi-algal culture; Growth inhibition
Strain(s): 10324 
DOI: 10.3354/meps339083

Nagasoe, S., Toda, S., Shimasaki, Y., Osima, Y., Uchida, T., Honjo, T. 2006 Growth inhibition of Gyrodinium instriatum (Dinophyceae) by Skeletonema costatum (Bacillariophyceae). Afr. J. Mar. Sci., 28, 325-329.
Keywords: allelopathy; Gyrodinium instriatum; Skeletonema costatum; species interaction
Strain(s): 324 
DOI: 10.2989/18142320609504171

Hirano, T., Masho, R., Koizumi, D., Tabata, S., Suzuki, K., Sakabe, M., Nakano, Y., Sato, N., Sudo, R. 2006 Ecotoxicity testing of bioremediation agents for shoreline oil-spill clean up. Jpn. J. Environ. Toxicol., 9, 115-131.
Keywords: ecotoxicity; bioremediation; oil-spill; acute toxicity; marine organisms
Strain(s): 324 
DOI: 10.11403/jset.9.115

Yamaguchi, H., Nishijima, T., Nishitani, H., Fukami, K., Adachi, M. 2004 Organic phosphorus utilization and alkaline phosphatase production of 3 red tide photoplankton. Nippon Suisan Gakkaishi, 70, 123-130 (in Japanese with English summary).
Keywords: Karenia mikimotoi; Skeletonema costatum; Heterosigma akashiwo; red tide phytoplankton; organic phosphorus; alkaline phosphatase
Strain(s): 5324 
DOI: 10.2331/suisan.70.123

Okumura, C., Hasegawa, H., Mizumoto, H., Maki, T., Ueda, K. 2004 Size fractionation of iron compounds in phytoplankton cultures in the presence of chelating ligands. Bunseki Kagaku, 53, 1215-1221 (in Japanese with English summary).
Keywords: iron; speciation; chelating ligands; phytoplankton growth
Strain(s): 324 
DOI: 10.2116/bunsekikagaku.53.1215

Okumura, Y., Koyama, J., Takaku, H., Satoh, H. 2001 Influence of organic solvents on the growth of marine microalgae. Arch. Environ. Contam. Toxicol., 41, 123-128.
Strain(s): 6324 
PubMed: 11462135
DOI: 10.1007/s002440010229

Mitsutani, A., Yamasaki, I., Kitaguchi, H., Kato, J., Ueno, S., Ishida, Y. 2001 Analysis of algicidal proteins of a diatom-lytic marine bacterium Pseudoalteromonas sp. strain A25 by two-dimensional electrophoresis. Phycologia, 40, 286-291.
Strain(s): 324 
DOI: 10.2216/i0031-8884-40-3-286.1

Lee, S, Kato, J, Takiguchi, N. Kuroda, A., Ikeda, T., Mitsutani, A., Ohtake, H. 2000 Involvement of an extracellular protease in algicidal activity of the marine bacterium Pseudoalteromonas sp. strain A28. Appl. Environ. Microbiol., 66, 4334-4339.
Strain(s): 324 
PubMed: 11010878
DOI: 10.1128/AEM.66.10.4334-4339.2000

Fukami, K., Matsumoto, J., Kadota, T., Nakano, Y. 2000 Seasonal fluctuations of water quality of deep sea water and the relationship to the growth potential of a microalga. Deep Ocean Water Research, 1, 27-31 (in Japanese with English summary).
Keywords: Deep seawater; water quality; inorganic nutrients; AGP; seasonal fluctuation; upwelling
Strain(s): 324 
DOI: 10.11174/dowas2000.1.1_27

Okamura, K. 1992 Growth characteristics of Skeletonema costatum for algal growth potential. Kanagawa-ken Kankyô Kagaku Sentâ Kenkyû Hôkoku, 15, 12-15 (in Japanese with English title).
Strain(s): 324 

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