A big image opens when the thumbnail is clicked.(nies-0323.jpg)
Strain number NIES-323  
Phylum Heterokontophyta  
Class Bacillariophyceae  
Scientific name Skeletonema marinoi-dohrnii complex  
Former name Skeletonema costatum (Greville) Cleve  
Common name Centric diatom  
Locality (Date of collection) Osaka Bay Off Kishiwada Osaka Japan (1985-01-21)  
Latitude / Longitude  
Habitat (Isolation source) Marine (Seawater)  
History < Osaka Pref. Fish. Exp. St.  
Isolator (Date of isolation) Yamochi, Susumu (1985-01-22)  
Identified by Yamochi, Susumu  
State of strain Subculture; Unialgal; Clonal; Axenic[2018 Feb]  
Culture condition
(Preculture condition)
Medium:  f/2  
Temperature:  10 C
Light intensity:  3-6 µmol photons/m2/sec, L/D cycle:  10L:14D
Duration:  1 M  
Gene information 18S rRNA ( AB488610 ) , psbC, psaD, petB, petD, ycf12, ycf9, ycf47 ( AJ132263 ) , ycf5, psaC, ycf35, psbA ( AJ132264 ) , ycf6, trnD, trnI, ycf33, trnT, ycf25 ( AJ132265 ) , ycf24 ( AJ132267 ) , ycf31, petG, psbK, psaI, psbD ( AJ132266 )  
Cell size (min - max) - 8 μm  
Organization Unicellular 
Characteristics Red tide ; Planktonic ; DNA sequencing indicates this strain is included in Skeletonema marinoi/dohrnii clade  
Other strain no. Other strain no. : Sk-85w  
Remarks Axenic 
Natsuike, M., Endo, Y., Ito, H., Miyamoto, M., Yoshimura, C., Fujii, M. 2020 Iron uptake kinetics by coastal micro- and macro-algae in relation to riverine and coastal organic matter. Estuar. Coast. Shelf Sci., 235, 106580 (article ID).
Keywords: Iron; Organic matter; Emission excitation matrix (EEM); Coastal waters; Fulvic acid
Strain(s): 323 
DOI: 10.1016/j.ecss.2019.106580

Mitani, E., Nakayama, F., Matsuwaki, I., Ichi, I., Kawabata, A., Kawachi, M., Kato, M. 2017 Fatty acid composition profiles of 235 strains of three microalgal divisions within the NIES Microbial Culture Collection. Microb. Resour. Syst., 33, 19-29.
Keywords: Cryptophyta; docosahexaenoic acid; eicosapentaenoic acid; fatty acid; Haptophyta; Heterokontophyta; microalgae
Strain(s): 1891415177111522322523323426527427527627727827928028128228429332332433033334434534634734835035337237738839139540740840941341441746146246648753454855355655755855956056258758858959060360562162262369569669769869970070170270370470570670770870971071171271371471571674176576676780583799710011002100310041005100610071009101110161017104410451046104713021303132413271330133913401349135313701375137613791383138413851386138713911392139313951398139914001401169917001730181318151816182618271831186218631864186518741963196419651974197519762142214321442145214721482300233123322351236323642365236923702376250625332534253525362537259026332668268926902691269326942696269727072716271727182720272227232725272627292730273127322770277127722773283928402841284228432844285928722878289028993391 

Yoshimura, T., Yanagawa, T., Fujiwara, A., Murai, K., Nogata, Y., Furuta, T., Kobayashi, T. 2016 Marine diatom Coscinodiscus wailesiii inhibits DPD measurement of residual chlorine in seawater samples. Sessile Organisms, 33, 38-43 (in Japanese with English summary).
Keywords: Residual chlorine; DPD; Seawater; Coscinodiscus wailesii
Strain(s): 15237323 
DOI: 10.4282/sosj.33.38

Okamura, H., Kitano, S., Toyota, S., Harino, H., Thomas, K. V. 2009 Ecotoxicity of the degradation products of triphenylborane pyridine (TPBP) antifouling agent. Chemosphere, 74, 1275-1278.
Keywords: Artemia; bioassay; biocide; biphenyl; marina; Skeletonema
Strain(s): 323 
PubMed: 19095285
DOI: 10.1016/j.chemosphere.2008.11.014

Hamana, K. 2008 Cellular polyamines of phototrophs and beterotrophs belonging to the lower eukaryotic phyla Cercozoa, Euglenozoa, Heterokonta and Metamonada. J. Gen. Appl. Microbiol., 54, 135-140.
Keywords: cyanobacteria; homospermidine; polyamine; spermine; thermospermine
Strain(s): 9157186118253323330333548603621622100210031011104413031380144014431444 
PubMed: 18497488
DOI: 10.2323/jgam.54.135

Kooistra, W. H. C. F., Sarno, D., Balzano, S., Gu, H., Andersen, R. A., Zingone, A. 2008 Global diversity and biogeography of Skeletonema species (Bacillariophyta). Protist, 159, 177-193.
Keywords: biogeography; cryptic species; distribution; LSU rDNA; phytoplankton; Skeletonema costatum
Strain(s): 1617223323324 
PubMed: 18042429
DOI: 10.1016/j.protis.2007.09.004

Fukao, T., Nishijima, T., Yamaguchi, H., Adachi, M. 2007 Utilization of urea by the 6 species of red tide phytoplankton. Bull. Plankton Soc. Japan, 54, 1-8 (in Japanese with English summary).
Keywords: growth; red tide; phytoplankton; urea; uptake kinetics
Strain(s): 16237323324605 

Tada, N., Shibata, S., Otsuka, S., Namba, K., Oyaizu, H. 1999 Comparison of gene arrangements of chloroplasts between two centric diatoms, Skeletonema costatum and Odontella sinensis. DNA Seq., 10, 343-347.
Keywords: chloroplast; diatom; gene arrangement; Skeletonema costatum
Strain(s): 323 

Itoh, Y., Murakami, H., Ikeda, R. 1993 Studies on the growth limiting nutrients of red tide plankton in the tidal area [Kanchô-iki ni okeru akashio gen'in sôrui no zôshoku yokusei inshi no suitei]. Proc. Coast. Engineering, JSCE [Kaigan Kôgaku Ronbun-shû], 40, 1001-1005 (in Japanese without English title).
Strain(s): 323 

Kohata, K., Watanabe, M., Yamanaka, K. 1991 Highly sensitive determination of photosynthetic pigments in marine in situ samples by high-performance liquid chromatography. J. Chromatogr. A 558, 131-140.
Strain(s): 67254323353 

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