Strain Data

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Strain number		NIES-323
Phylum		Heterokontophyta
Class		Bacillariophyceae
Scientific name		Skeletonema marinoi-dohrnii complex
Synonym
Former name		Skeletonema costatum (Greville) Cleve
Common name		Centric diatom
Locality (Date of collection)		Osaka Bay, Off Kishiwada, Osaka, Japan (1985-01-21)
Latitude / Longitude
Habitat (Isolation source)		Marine (Seawater)
History		< Osaka Pref. Fish. Exp. St.
Isolator (Date of isolation)		Yamochi, Susumu (1985-01-22)
Identified by		Yamochi, Susumu
State of strain		Subculture; Unialgal; Clonal; Axenic[2018 Feb]
Culture condition (Preculture condition)		Medium: f/2 Temperature: 15 C Light intensity: 8 µmol photons/m²/sec, L/D cycle: 10L:14D Duration: 1 M
Gene information		18S rRNA ( AB488610 ) , psbC, psaD, petB, petD, ycf12, ycf9, ycf47 ( AJ132263 ) , ycf5, psaC, ycf35, psbA ( AJ132264 ) , ycf6, trnD, trnI, ycf33, trnT, ycf25 ( AJ132265 ) , ycf24 ( AJ132267 ) , ycf31, petG, psbK, psaI, psbD ( AJ132266 )
Cell size (min - max)		- 8 μm
Organization		Unicellular
Characteristics		Red tide ; Planktonic ; DNA sequencing indicates this strain is included in Skeletonema marinoi/dohrnii clade
Other strain no.		Other strain no. : Sk-85w
Remarks		Axenic
Movie

Reference
Takeda, S. 2024 Growth responses of coastal phytoplankton to continuous supply of low nitric oxide concentrations during the light period. Bull. Fac. Fish., Nagasaki Univ., 104, 1-10. Keywords: phytoplankton; nitric oxide; growth; fed-batch culture; coastal waters Strain(s): 113, 323 Natsuike, M., Endo, Y., Ito, H., Miyamoto, M., Yoshimura, C., Fujii, M. 2020 Iron uptake kinetics by coastal micro- and macro-algae in relation to riverine and coastal organic matter. Estuar. Coast. Shelf Sci., 235, 106580 (article ID). Keywords: Iron; Organic matter; Emission excitation matrix (EEM); Coastal waters; Fulvic acid Strain(s): 323 DOI: 10.1016/j.ecss.2019.106580 Yamagishi, T., Ota, S., Yamaguchi, H., Koshikawa, H., Tatarazako, N., Yamamoto, H., Kawachi, M. 2019 Ecotoxicological bioassay using marine algae for deep-sea mining. In Environmental Issues of Deep-Sea Mining: Impacts, Consequences and Policy Perspectives, Ed. by Sharma, R., Springer, Cham, pp. 255-271. Strain(s): 323, 613, 969, 971, 981, 1000, 1001, 1310, 1411, 2304, 2587, 2590, 2670, 2672 DOI: 10.1007/978-3-030-12696-4_10 Mitani, E., Nakayama, F., Matsuwaki, I., Ichi, I., Kawabata, A., Kawachi, M., Kato, M. 2017 Fatty acid composition profiles of 235 strains of three microalgal divisions within the NIES Microbial Culture Collection. Microb. Resour. Syst., 33, 19-29. Keywords: Cryptophyta; docosahexaenoic acid; eicosapentaenoic acid; fatty acid; Haptophyta; Heterokontophyta; microalgae Strain(s): 1, 8, 9, 14, 15, 17, 71, 115, 223, 225, 233, 234, 265, 274, 275, 276, 277, 278, 279, 280, 281, 282, 284, 293, 323, 324, 330, 333, 344, 345, 346, 347, 348, 350, 353, 372, 377, 388, 391, 395, 407, 408, 409, 413, 414, 417, 461, 462, 466, 487, 534, 548, 553, 556, 557, 558, 559, 560, 562, 587, 588, 589, 590, 603, 605, 622, 623, 695, 696, 697, 698, 699, 700, 701, 702, 703, 704, 705, 706, 707, 708, 709, 710, 711, 712, 713, 714, 715, 716, 741, 765, 766, 767, 805, 837, 997, 1001, 1002, 1003, 1004, 1005, 1006, 1007, 1009, 1011, 1016, 1017, 1044, 1045, 1046, 1047, 1302, 1303, 1324, 1327, 1330, 1339, 1340, 1349, 1353, 1370, 1375, 1376, 1379, 1383, 1384, 1385, 1386, 1387, 1391, 1392, 1393, 1395, 1398, 1399, 1400, 1401, 1699, 1700, 1730, 1813, 1815, 1816, 1826, 1827, 1831, 1862, 1863, 1864, 1865, 1874, 1963, 1964, 1965, 1974, 1975, 1976, 2142, 2143, 2144, 2145, 2147, 2148, 2300, 2331, 2332, 2351, 2363, 2364, 2365, 2369, 2370, 2376, 2506, 2533, 2534, 2535, 2536, 2537, 2590, 2633, 2668, 2689, 2690, 2691, 2693, 2694, 2696, 2697, 2707, 2716, 2717, 2718, 2720, 2722, 2723, 2725, 2726, 2729, 2730, 2731, 2732, 2770, 2771, 2772, 2773, 2839, 2840, 2841, 2842, 2843, 2844, 2859, 2872, 2878, 2890, 2899, 3391, 3689, 3690, 3691 Yoshimura, T., Yanagawa, T., Fujiwara, A., Murai, K., Nogata, Y., Furuta, T., Kobayashi, T. 2016 Marine diatom Coscinodiscus wailesii inhibits DPD measurement of residual chlorine in seawater samples. Sessile Organisms, 33, 38-43 (in Japanese with English summary). Keywords: Residual chlorine; DPD; Seawater; Coscinodiscus wailesii Strain(s): 1, 5, 237, 323 DOI: 10.4282/sosj.33.38 Okamura, H., Kitano, S., Toyota, S., Harino, H., Thomas, K. V. 2009 Ecotoxicity of the degradation products of triphenylborane pyridine (TPBP) antifouling agent. Chemosphere, 74, 1275-1278. Keywords: Artemia; bioassay; biocide; biphenyl; marina; Skeletonema Strain(s): 323 PubMed: 19095285 DOI: 10.1016/j.chemosphere.2008.11.014 Hamana, K. 2008 Cellular polyamines of phototrophs and beterotrophs belonging to the lower eukaryotic phyla Cercozoa, Euglenozoa, Heterokonta and Metamonada. J. Gen. Appl. Microbiol., 54, 135-140. Keywords: cyanobacteria; homospermidine; polyamine; spermine; thermospermine Strain(s): 9, 15, 71, 86, 118, 253, 323, 330, 333, 548, 603, 622, 1002, 1003, 1011, 1044, 1303, 1380, 1440, 1443, 1444 PubMed: 18497488 DOI: 10.2323/jgam.54.135 Kooistra, W. H. C. F., Sarno, D., Balzano, S., Gu, H., Andersen, R. A., Zingone, A. 2008 Global diversity and biogeography of Skeletonema species (Bacillariophyta). Protist, 159, 177-193. Keywords: biogeography; cryptic species; distribution; LSU rDNA; phytoplankton; Skeletonema costatum Strain(s): 16, 17, 223, 323, 324 PubMed: 18042429 DOI: 10.1016/j.protis.2007.09.004 Fukao, T., Nishijima, T., Yamaguchi, H., Adachi, M. 2007 Utilization of urea by the 6 species of red tide phytoplankton. Bull. Plankton Soc. Japan, 54, 1-8 (in Japanese with English summary). Keywords: growth; red tide; phytoplankton; urea; uptake kinetics Strain(s): 1, 6, 237, 323, 324, 605 Ohnishi, Y., Fujii, M., Murashige, S., Yuzawa, A., Miyasaka, H., Suzuki, Y. 2002 Decomposition precess of organic matter in sea water using phytoplankton. I. Effect of the difference in initial concentration of organic matter. Bull. Soc. Sea Water Sci. Jpn., 56, 17-25 (in Japanese with English summary). Keywords: Initial concentration; Decomposition preocess: Phytoplankton; Particulate organic matter; Dissolved organic matter Strain(s): 323 DOI: 10.11457/swsj1965.56.17 Tada, N., Shibata, S., Otsuka, S., Namba, K., Oyaizu, H. 1999 Comparison of gene arrangements of chloroplasts between two centric diatoms, Skeletonema costatum and Odontella sinensis. DNA Seq., 10, 343-347. Keywords: chloroplast; diatom; gene arrangement; Skeletonema costatum Strain(s): 323 PubMed: 10727090 DOI: 10.3109/10425179909033962 Itoh, Y., Murakami, H., Ikeda, R. 1993 Studies on the growth limiting nutrients of red tide plankton in the tidal area [Kanchô-iki ni okeru akashio gen'in sôrui no zôshoku yokusei inshi no suitei]. Proc. Coast. Engineering, JSCE [Kaigan Kôgaku Ronbun-shû], 40, 1001-1005 (in Japanese without English title). Strain(s): 323 Kohata, K., Watanabe, M., Yamanaka, K. 1991 Highly sensitive determination of photosynthetic pigments in marine in situ samples by high-performance liquid chromatography. J. Chromatogr. A 558, 131-140. Strain(s): 6, 7, 254, 323, 353 DOI: 10.1016/0021-9673(91)80118-Z

Reference

Takeda, S. 2024 Growth responses of coastal phytoplankton to continuous supply of low nitric oxide concentrations during the light period. Bull. Fac. Fish., Nagasaki Univ., 104, 1-10.
Keywords: phytoplankton; nitric oxide; growth; fed-batch culture; coastal waters
Strain(s): 113, 323

Natsuike, M., Endo, Y., Ito, H., Miyamoto, M., Yoshimura, C., Fujii, M. 2020 Iron uptake kinetics by coastal micro- and macro-algae in relation to riverine and coastal organic matter. Estuar. Coast. Shelf Sci., 235, 106580 (article ID).
Keywords: Iron; Organic matter; Emission excitation matrix (EEM); Coastal waters; Fulvic acid
Strain(s): 323
DOI: 10.1016/j.ecss.2019.106580

Yamagishi, T., Ota, S., Yamaguchi, H., Koshikawa, H., Tatarazako, N., Yamamoto, H., Kawachi, M. 2019 Ecotoxicological bioassay using marine algae for deep-sea mining. In Environmental Issues of Deep-Sea Mining: Impacts, Consequences and Policy Perspectives, Ed. by Sharma, R., Springer, Cham, pp. 255-271.
Strain(s): 323, 613, 969, 971, 981, 1000, 1001, 1310, 1411, 2304, 2587, 2590, 2670, 2672
DOI: 10.1007/978-3-030-12696-4_10

Mitani, E., Nakayama, F., Matsuwaki, I., Ichi, I., Kawabata, A., Kawachi, M., Kato, M. 2017 Fatty acid composition profiles of 235 strains of three microalgal divisions within the NIES Microbial Culture Collection. Microb. Resour. Syst., 33, 19-29.
Keywords: Cryptophyta; docosahexaenoic acid; eicosapentaenoic acid; fatty acid; Haptophyta; Heterokontophyta; microalgae
Strain(s): 1, 8, 9, 14, 15, 17, 71, 115, 223, 225, 233, 234, 265, 274, 275, 276, 277, 278, 279, 280, 281, 282, 284, 293, 323, 324, 330, 333, 344, 345, 346, 347, 348, 350, 353, 372, 377, 388, 391, 395, 407, 408, 409, 413, 414, 417, 461, 462, 466, 487, 534, 548, 553, 556, 557, 558, 559, 560, 562, 587, 588, 589, 590, 603, 605, 622, 623, 695, 696, 697, 698, 699, 700, 701, 702, 703, 704, 705, 706, 707, 708, 709, 710, 711, 712, 713, 714, 715, 716, 741, 765, 766, 767, 805, 837, 997, 1001, 1002, 1003, 1004, 1005, 1006, 1007, 1009, 1011, 1016, 1017, 1044, 1045, 1046, 1047, 1302, 1303, 1324, 1327, 1330, 1339, 1340, 1349, 1353, 1370, 1375, 1376, 1379, 1383, 1384, 1385, 1386, 1387, 1391, 1392, 1393, 1395, 1398, 1399, 1400, 1401, 1699, 1700, 1730, 1813, 1815, 1816, 1826, 1827, 1831, 1862, 1863, 1864, 1865, 1874, 1963, 1964, 1965, 1974, 1975, 1976, 2142, 2143, 2144, 2145, 2147, 2148, 2300, 2331, 2332, 2351, 2363, 2364, 2365, 2369, 2370, 2376, 2506, 2533, 2534, 2535, 2536, 2537, 2590, 2633, 2668, 2689, 2690, 2691, 2693, 2694, 2696, 2697, 2707, 2716, 2717, 2718, 2720, 2722, 2723, 2725, 2726, 2729, 2730, 2731, 2732, 2770, 2771, 2772, 2773, 2839, 2840, 2841, 2842, 2843, 2844, 2859, 2872, 2878, 2890, 2899, 3391, 3689, 3690, 3691

Yoshimura, T., Yanagawa, T., Fujiwara, A., Murai, K., Nogata, Y., Furuta, T., Kobayashi, T. 2016 Marine diatom Coscinodiscus wailesii inhibits DPD measurement of residual chlorine in seawater samples. Sessile Organisms, 33, 38-43 (in Japanese with English summary).
Keywords: Residual chlorine; DPD; Seawater; Coscinodiscus wailesii
Strain(s): 1, 5, 237, 323
DOI: 10.4282/sosj.33.38

Okamura, H., Kitano, S., Toyota, S., Harino, H., Thomas, K. V. 2009 Ecotoxicity of the degradation products of triphenylborane pyridine (TPBP) antifouling agent. Chemosphere, 74, 1275-1278.
Keywords: Artemia; bioassay; biocide; biphenyl; marina; Skeletonema
Strain(s): 323
PubMed: 19095285
DOI: 10.1016/j.chemosphere.2008.11.014

Hamana, K. 2008 Cellular polyamines of phototrophs and beterotrophs belonging to the lower eukaryotic phyla Cercozoa, Euglenozoa, Heterokonta and Metamonada. J. Gen. Appl. Microbiol., 54, 135-140.
Keywords: cyanobacteria; homospermidine; polyamine; spermine; thermospermine
Strain(s): 9, 15, 71, 86, 118, 253, 323, 330, 333, 548, 603, 622, 1002, 1003, 1011, 1044, 1303, 1380, 1440, 1443, 1444
PubMed: 18497488
DOI: 10.2323/jgam.54.135

Kooistra, W. H. C. F., Sarno, D., Balzano, S., Gu, H., Andersen, R. A., Zingone, A. 2008 Global diversity and biogeography of Skeletonema species (Bacillariophyta). Protist, 159, 177-193.
Keywords: biogeography; cryptic species; distribution; LSU rDNA; phytoplankton; Skeletonema costatum
Strain(s): 16, 17, 223, 323, 324
PubMed: 18042429
DOI: 10.1016/j.protis.2007.09.004

Fukao, T., Nishijima, T., Yamaguchi, H., Adachi, M. 2007 Utilization of urea by the 6 species of red tide phytoplankton. Bull. Plankton Soc. Japan, 54, 1-8 (in Japanese with English summary).
Keywords: growth; red tide; phytoplankton; urea; uptake kinetics
Strain(s): 1, 6, 237, 323, 324, 605

Ohnishi, Y., Fujii, M., Murashige, S., Yuzawa, A., Miyasaka, H., Suzuki, Y. 2002 Decomposition precess of organic matter in sea water using phytoplankton. I. Effect of the difference in initial concentration of organic matter. Bull. Soc. Sea Water Sci. Jpn., 56, 17-25 (in Japanese with English summary).
Keywords: Initial concentration; Decomposition preocess: Phytoplankton; Particulate organic matter; Dissolved organic matter
Strain(s): 323
DOI: 10.11457/swsj1965.56.17

Tada, N., Shibata, S., Otsuka, S., Namba, K., Oyaizu, H. 1999 Comparison of gene arrangements of chloroplasts between two centric diatoms, Skeletonema costatum and Odontella sinensis. DNA Seq., 10, 343-347.
Keywords: chloroplast; diatom; gene arrangement; Skeletonema costatum
Strain(s): 323
PubMed: 10727090
DOI: 10.3109/10425179909033962

Itoh, Y., Murakami, H., Ikeda, R. 1993 Studies on the growth limiting nutrients of red tide plankton in the tidal area [Kanchô-iki ni okeru akashio gen'in sôrui no zôshoku yokusei inshi no suitei]. Proc. Coast. Engineering, JSCE [Kaigan Kôgaku Ronbun-shû], 40, 1001-1005 (in Japanese without English title).
Strain(s): 323

Kohata, K., Watanabe, M., Yamanaka, K. 1991 Highly sensitive determination of photosynthetic pigments in marine in situ samples by high-performance liquid chromatography. J. Chromatogr. A 558, 131-140.
Strain(s): 6, 7, 254, 323, 353
DOI: 10.1016/0021-9673(91)80118-Z

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