Strain number NIES-4284  
Phylum Chlorophyta  
Class Chlorophyceae  
Scientific name Chloromonas muramotoi Matsuzaki, Nozaki & Kawachi  
Former name  
Common name Green alga ; Snow alga  
Locality (Date of collection) the Hakkoda Botanical Garden of Tohoku University, Mt. Hakkoda, Aomori, Aomori, Japan (2012-05-16)  
Latitude / Longitude 40.646944 / 140.851389 
Habitat (Isolation source) Terrestrial (Snow)  
History < Matsuzaki, Ryo  
Isolator (Date of isolation) Matsuzaki, Ryo (2012-03-23)  
Identified by Matsuzaki, Ryo  
State of strain Subculture; Unialgal; Clonal; Non-axenic  
Culture condition
(Preculture condition)
Medium:  AF-6  
Temperature:  5 C
Light intensity:  51 µmol photons/m2/sec, L/D cycle:  10L:14D
Duration:  3 M  
Gene information 18S rRNA ( LC438435 ) , 28S rRNA ( LC438439 ) , atpB ( LC438443 ) , psaB ( LC438447 ) , rbcL ( LC438451 ) , ITS2 ( LC438455 )  
Cell size (min - max) 12 - 19 μm  
Organization Unicellular; Flagellate 
Characteristics Cryophilic ; Authentic strain (Matsuzaki et al. 2019)  
Other strain no. Other strain no. : HkCl-57  
Matsuzaki, R., Suzuki, S., Yamaguchi, H., Kawachi, M., Kanesaki, Y., Yoshikawa, H., Mori, T., Nozaki, H. 2021 The Rubisco small subunits in the green algal genus Chloromonas provide insights into evolutionary loss of the eukaryotic carbon-concentrating organelle, the pyrenoid. BMC Ecol. Evol., 21, 11 (article ID).
Keywords: Chloromonas; Evolution; Green algae; Hydrophobicity of RBCS helices; Pyrenoid; Pyrenoid loss; Rubisco small subunit (RBCS)
Strain(s): 14442442522072212221522422243249937534284 
PubMed: 33514317
DOI: 10.1186/s12862-020-01733-1

Matsuzaki, R., Nozaki, H., Takeuchi, N., Hara, Y., Kawachi, M. 2019 Taxonomic re-examination of “Chloromonas nivalis (Volvocales, Chlorophyceae) zygotes” from Japan and description of C. muramotoi sp. nov. PLoS One, 14, e0210986 (article ID).
Strain(s): 46423792380256328623389370737534284 
PubMed: 30677063
DOI: 10.1371/journal.pone.0210986

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