Strain number NIES-4114  
Phylum Stramenopila incertae sedis  
Class Incertae sedis  
Scientific name Pseudophyllomitus vesiculosus (Larsen & Patterson) Lee  
Former name  
Common name  
Locality (Date of collection) Kasari Bay Amami, Kagoshima Japan (2015-01-22)  
Latitude / Longitude 28.441809 / 129.671856 
Habitat (Isolation source) Marine (Sediment)  
History < Shiratori, Takashi; Ishida, Ken-ichiro  
Isolator (Date of isolation) Shiratori, Takashi (2015-01-29)  
Identified by Shiratori, Takashi  
State of strain Subculture; Mixed (Entomoeis sp. and Bacterium as food); Clonal; Non-axenic  
Culture condition
(Preculture condition)
Medium:  ESM  
Temperature:  20 C
Light intensity:  4 µmol photons/m2/sec, L/D cycle:  10L:14D
Duration:  1 M  
Gene information 18S rRNA ( LC257669 )  
Cell size (min - max) 6 - 12 μm  
Organization Unicellular; Flagellate 
Characteristics Heterotrophic ; Benthic ; Authentic strain (Shiratori et al. 2017)  
Other strain no. Other strain no. : SRT537  
Thakur, R., Shiratori, T., Ishida, K-I. 2019 Taxon-rich multigene phylogenetic analyses resolve the phylogenetic relationship among deep-branching Stramenopiles. Protist, 5, 125682 (article ID).
Keywords: Stramenopiles; evolution; phylogeny; transcriptome data; MAST
Strain(s): 4114 
PubMed: 31568885
DOI: 10.1016/j.protis.2019.125682

Shiratori, T., Thakur, R., Ishida, K-I. 2017 Pseudophyllomitus vesiculosus (Larsen and Patterson 1990) Lee, 2002, a poorly studied phagotrophic biflagellate is the first characterized member of Stramenopile environmental clade MAST-6. Protist, 168, 439-451.
Keywords: Flagellar apparatus; MAST; phylogenetic analysis; stramenopiles; ultrastructure
Strain(s): 4114 
PubMed: 28822908
DOI: 10.1016/j.protis.2017.06.004

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