Strain number NIES-4107  
Phylum Chlorophyta  
Class Chlorophyceae  
Scientific name Gymnomonas nepalensis Shin Watanabe  
Former name Dunaliella lateralis Pascher & Jahoda  
Common name Green alga  
Locality (Date of collection) near Bargu, Nepal (1975-10-09)  
Latitude / Longitude  
Habitat (Isolation source) Terrestrial (NIES-3572)  
History < Nakada, Takashi < Watanabe, Shin  
Isolator (Date of isolation) Nakada, Takashi (2016-04-19)  
Identified by Watanabe, Shin  
State of strain Cryopreservation; Unialgal; Clonal; Axenic[2020 Sept]  
Culture condition
(Preculture condition)
Medium:  AF-6  
Temperature:  20 C
Light intensity:  25 µmol photons/m2/sec, L/D cycle:  10L:14D
Duration:  2 M  
Gene information 18S rRNA ( DQ009762 LC268992 ) , 26S rRNA ( DQ015741 ) , ITS1-5.8S-ITS2 ( DQ377089 ) , ITS1 ( AF313444 ) , ITS2 ( AF313445 ) , atpB ( LC268993 ) , psaB ( LC268994 ) , rbcL ( LC268995 )  
Cell size (min - max)  
Organization Unicellular; Flagellate 
Characteristics Authentic strain (Watanabe & Nakada 2018) ; Type specimen (NIES-50015, Holotype)  
Other strain no. Other strain no. : NIES-3572P1  
Remarks Cryopreserved; Purified strain from NIES-3572; Axenic 
Hamana, K., Furuchi, T., Hayashi, H., Uemura, T., Niitsu, M. 2023 Additional polyamine analysis of the algae belonging to the phyla Glaucophyta, Rhodophyta and Chlorophyta. ─Polyamine analysis of algae V─ Microb. Resour. Syst., 39, 33-40.
Keywords: alga; Chlorophyta; Glaucophyta; polyamine; Rhodophyta
Strain(s): 22625025455055162612981332170318362137216321642165216622522353289433773645386738913892410743904452 

Watanabe, S. & Nakada, T. 2018 Gymnomonas nepalensis gen. et sp. nov. for the naked flagellate strain ‘Nepal’, formerly identified as Dunaliella lateralis (Volvocales, Chlorophyceae). Phycol. Res., 66, 167-172.
Strain(s): 35724107 
DOI: 10.1111/pre.12219

González, M. A., Coleman, A. W., Gómez, P. I., Montoya, R. 2001 Phylogenetic relationship among various strains of Dunaliella (Chlorophyceae) based on nuclear ITS rDNA sequences. J. Phycol., 37, 604-611.
Keywords: Dunaliella; internal transcribed spacer; phylogenetics; rDNA; sequencing; strains; taxonomy
Strain(s): 35724107 
DOI: 10.1046/j.1529-8817.2001.037004604.x

Watanabe, S. 1983 New and interesting green algae from soils of some Asian and Oceanian regions. Arch. Protistenk., 127, 223-270.
Strain(s): 153154155157158159160357235734107 
DOI: 10.1016/S0003-9365(83)80021-9

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