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Strain number NIES-325  
Phylum Streptophyta  
Class Zygnematophyceae  
Scientific name Spinoclosterium cuspidatum (Bailey) Hirano  
Synonym Closterium cuspidatum Bailey; Ophiocytium cuspidatum (Bailey) Rabenhorst 1868; Tetraëdron cuspidatum (Bailey) Wille ex Brunnthaler 1915; Spinoclosterium curvatum var. spinosum Prescott  
Former name  
Common name Desmid  
Locality (Date of collection) Higashihiroshima, Hiroshima, Japan (1983-10-19)  
Latitude / Longitude 34.448135 / 132.760949 
Habitat (Isolation source) Freshwater (Pond water)  
History < Ichimura, Terunobu  
Isolator (Date of isolation) Ichimura, Terunobu (1983-10-**)  
Identified by Ichimura, Terunobu  
State of strain Subculture; Unialgal; Clonal; Non-axenic  
Culture condition
(Preculture condition)
Medium:  SW  
Temperature:  20 (25) C
Light intensity:  6-9 (14-16) µmol photons/m2/sec, L/D cycle:  10L:14D
Duration:  8 M (1 M)  
Gene information 18S rRNA ( AJ553931 ) , 23S rRNA ( FM992543 ) , CO3 ( EF371132 ) , psaA ( EF371240 ) , rbcL ( AJ553965 )  
Cell size (min - max) - 100 μm  
Organization Unicellular 
Characteristics Homothallic  
Other strain no. Other strain no. : 83-24-19  
Ichimura, T. & Kasai, F. 1985 Studies on the life cycle of Spinoclosterium cuspidatum (Bailey) Hirano (Conjugatophyceae). Phycologia, 24, 205-216.
Strain(s): 325 
DOI: 10.2216/i0031-8884-24-2-205.1

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