Strain number NIES-4068  
Phylum Chlorophyta  
Class Chlorophyceae  
Scientific name Volvox tertius Art.Meyer  
Synonym Merillosphaera tertia (Art.Meyer) W.R.Shaw 1922; Heterotypic synonyms; Volvox bernardii Playfair null; Volvox mononae G.M.Smith 1920; Merrillosphaera migulae W.R.Shaw 1922  
Former name  
Common name Green alga ; Volvocine alga  
Locality (Date of collection) Ninepipe Reservoir, Montana, USA (2014-07-28)  
Latitude / Longitude 47.45541667 /  
Habitat (Isolation source) Freshwater (Reservoir water)  
History < Nozaki, Hisayoshi < Yamamoto, Kayoko  
Isolator (Date of isolation) Yamamoto, Kayoko (2014-07-28)  
Identified by Nozaki, Hisayoshi  
State of strain Subculture; Unialgal; Clonal; Axenic[2017 May]  
Culture condition
(Preculture condition)
Medium:  AF-6VTAC  
Temperature:  20 C
Light intensity:  23 µmol photons/m2/sec, L/D cycle:  10L:14D
Duration:  1 M  
Gene information rbcL ( LC215631 ) , atpB ( LC215630 ) , psab ( LC215629 ) , psaA ( LC215628 ) , psbC ( LC215627 ) , ITS1-5.8S-ITS2 ( LC215411 )  
Cell size (min - max)  
Organization Colonial; Flagellate 
Characteristics Phototaxis ; Homothallic ; Dioecious  
Other strain no. Other strain no. : 1-3 k-4  
Remarks Fragile species to transportation stresses; Read and agree "How to order 4.1"; Axenic 
Takahashi, K., Kawai-Toyooka, H., Ootsuki, R., Hamaji, T., Tsuchikane, Y., Sekimoto, H., Higashiyama, T., Nozaki, H. 2021 Three sex phenotypes in a haploid algal species give insights into the evolutionary transition to a self-compatible mating system. Evolution, 75, 2984-2993.
Keywords: Haploid species; inbreeding; life-history evolution; mating systems; reproductive isolation; sex
Strain(s): 456460541544731732872891892136213632569338233833780378240664068447944804481448244834484 
PubMed: 34250602
DOI: 10.1111/evo.14306

Nozaki. H., Mahakham, W., Athibai, S., Yamamoto, K., Takusagawa, M., Misumi, O., Herron, M. D., Rosenzweig, F., Kawachi, M. 2017 Rediscovery of the species of ‘ancestral Volvox’: morphology and phylogenetic position of Pleodorina sphaerica (Volvocales, Chlorophyceae) from Thailand. Phycologia, 56, 469-475.
Keywords: Molecular phylogeny; Morphology; Pleodorina; Pleodorina sphaerica; Sexuality; Somatic cell; Volvox
Strain(s): 4066406740684069 
PubMed: 29375162
DOI: 10.2216/17-3.1

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