Strain number NIES-4066  
Phylum Chlorophyta  
Class Chlorophyceae  
Scientific name Pleodorina sphaerica M.O.P.Iyengar  
Synonym Eudorina sphaerica (Iyengar) Goldstein 1964  
Former name  
Common name Green alga  
Locality (Date of collection) Phon Thong Lake, Phon Thong District, Roi Et Province, Thailand (2015-11-28)  
Latitude / Longitude 16.27869444 / 104.00000556 
Habitat (Isolation source) Freshwater (Lake water)  
History < Nozaki, Hisayoshi  
Isolator (Date of isolation) Nozaki, Hisayoshi (2015-11-28)  
Identified by Nozaki, Hisayoshi  
State of strain Subculture; Unialgal; Clonal; Axenic[2017 May]  
Culture condition
(Preculture condition)
Medium:  AF-6AF-6/3  
Temperature:  20 C
Light intensity:  20 µmol photons/m2/sec, L/D cycle:  10L:14D
Duration:  1 M  
Gene information psaA ( LC215637 ) , atpB ( LC215636 ) , psbC ( LC215635 ) , rbcL ( LC215634 ) , psaB ( LC215633 )  
Cell size (min - max)  
Organization Colonial; Flagellate 
Characteristics Phototaxis ; Homothallic ; Dioecious  
Other strain no. Other strain no. : 2015-1128-2P-12  
Remarks Axenic 
Movie  
Reference
Takahashi, K., Kawai-Toyooka, H., Ootsuki, R., Hamaji, T., Tsuchikane, Y., Sekimoto, H., Higashiyama, T., Nozaki, H. 2021 Three sex phenotypes in a haploid algal species give insights into the evolutionary transition to a self-compatible mating system. Evolution, 75, 2984-2993.
Keywords: Haploid species; inbreeding; life-history evolution; mating systems; reproductive isolation; sex
Strain(s): 456460541544731732872891892136213632569338233833780378240664068447944804481448244834484 
PubMed: 34250602
DOI: 10.1111/evo.14306

Nozaki. H., Mahakham, W., Athibai, S., Yamamoto, K., Takusagawa, M., Misumi, O., Herron, M. D., Rosenzweig, F., Kawachi, M. 2017 Rediscovery of the species of ‘ancestral Volvox’: morphology and phylogenetic position of Pleodorina sphaerica (Volvocales, Chlorophyceae) from Thailand. Phycologia, 56, 469-475.
Keywords: Molecular phylogeny; Morphology; Pleodorina; Pleodorina sphaerica; Sexuality; Somatic cell; Volvox
Strain(s): 4066406740684069 
PubMed: 29375162
DOI: 10.2216/17-3.1

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