Strain number NIES-4034  
Phylum Chlorophyta  
Class Trebouxiophyceae  
Scientific name Carolibrandtia ciliaticola (Hoshina, Kobayashi, Suzaki & Kusuoka) Hoshina & Nakada  
Synonym Brandtia ciliaticola Hoshina, Kobayashi, Suzaki & Kusuoka  
Former name  
Common name Green alga  
Locality (Date of collection) Lake Biwa, Yanagawa, Hikone, Shiga, Japan (2016-03-30)  
Latitude / Longitude 35.2290592 / 136.1537821 
Habitat (Isolation source) Freshwater (Stokesia vernalis)  
History < Hoshina, Ryo  
Isolator (Date of isolation) Hoshina, Ryo (2016-05-30)  
Identified by Hoshina, Ryo  
State of strain Cryopreservation; Unialgal; Non-axenic[2017 May]  
Culture condition
(Preculture condition)
Medium:  CAFAC  
Temperature:  20 C
Light intensity:  14-18 µmol photons/m2/sec, L/D cycle:  10L:14D
Duration:  2 M  
Gene information 18S-ITS1-5.8S-ITS2 ( LC228606 )  
Cell size (min - max) 3 - 5 μm  
Characteristics Symbiotic  
Other strain no. Other strain no. : 331StokesiaA  
Remarks Cryopreserved 
Krivina, E., Portnov, A., Temraleeva, A. 2024 A description of Aliichlorella ignota gen. et sp. nov. and a comparison of the efficiency of species delimitation methods in the Chlorella-clade (Trebouxiophyceae, Chlorophyta). Phycol. Res., ,
Strain(s): 4552167217121732330403240334034 
DOI: 10.1111/pre.12551

Krivina, E. S. & Temraleeva, A. D. 2020 Identification problems and cryptic diversity of Chlorella-clade microalgae (Chlorophyta). Microbiology, 89, 720-732.
Keywords: microalgae; morphology; ecology; phylogeny; 18S−ITS1−5.8S−ITS2 fragments; polyphasic approach
Strain(s): 2167217340324034 
DOI: 10.1134/S0026261720060107

Hoshina, R., Kobayashi, M., Suzaki, T., Kusuoka, Y. 2018 Brandtia ciliaticola gen. et sp. nov. (Chlorellaceae, Trebouxiophyceae) a common symbiotic green coccoid of various ciliate species. Phycol. Res., 66, 76-81.
Keywords: Chlorella; genus definition; rRNA structure
Strain(s): 403240334034 
DOI: 10.1111/pre.12194

Hoshina, R. & Nakada, T. 2018 Carolibrandtia nom. nov. as a replacement name for Brandtia Hoshina (Chlorellaceae, Trebouxiophyceae). Phycol. Res., 66, 82-83.
Keywords: Chlorella-like alga; ciliate; later homonym; nomenclature; photobiont; symbiosis
Strain(s): 403240334034 
DOI: 10.1111/pre.12208

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