Strain number NIES-4027  
Phylum Chlorophyta  
Class Chlorophyceae  
Scientific name Volvox sp.  
Former name  
Common name Green alga ; Volvocine alga  
Locality (Date of collection) Lake Sagami, Kanagawa, Japan (2015-06-10)  
Latitude / Longitude 35.610417 / 139.185833 
Habitat (Isolation source) Freshwater (Lake water)  
History < Saigo, Tokiko; Nozaki, Hisayoshi  
Isolator (Date of isolation) Saigo, Tokiko (2015-06-10)  
Identified by Nozaki, Hisayoshi  
State of strain Subculture; Unialgal; Clonal; Non-axenic[2018 Feb]  
Culture condition
(Preculture condition)
Medium:  AF-6AF-6/2  
Temperature:  20 C
Light intensity:  24-26 µmol photons/m2/sec, L/D cycle:  10L:14D
Duration:  1 M  
Gene information ITS1-5.8S-ITS2 ( LC191313 ) , rbcL ( LC191321 ) , psbC ( LC191331 )  
Cell size (min - max) 10 - 60 μm  
Organization Colonial; Flagellate 
Characteristics Other water bloom ; Phototaxis ; Endemic to Japan ; Homothallic ; Oogamy ; Haploid life cycle ; Resting zygote forming  
Other strain no. Other strain no. : 15-Sagami8-2  
Remarks Fragile species to transportation stresses; Read and agree "How to order 4.1" 
Nozaki, H., Ueki, N., Isaka, N., Saigo, T., Yamamoto, K., Matsuzaki, R., Takahashi, F., Wakabayashi, K-I., Kawachi, M. 2016 A new morphological type of Volvox from japanese large lakes and recent divergence of this type and V. ferrisii in two different freshwater habitats. PLoS One, 11, e0167148 (article ID).
Strain(s): 273627373987398840214022402340244025402640274028 
PubMed: 27880842
DOI: 10.1371/journal.pone.0167148

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