Strain number NIES-4022  
Phylum Chlorophyta  
Class Chlorophyceae  
Scientific name Volvox sp. Sagami  
Synonym  
Former name  
Common name Green alga ; Volvocine alga  
Locality (Date of collection) Lake Sagami, Kanagawa, Japan (2013-06-14)  
Latitude / Longitude 35.6093 / 139.188 
Habitat (Isolation source) Freshwater (Lake water)  
History < Nozaki, Hisayoshi  
Isolator (Date of isolation) Nozaki, Hisayoshi (2013-06-14)  
Identified by Nozaki, Hisayoshi  
State of strain Subculture; Unialgal; Clonal; Axenic[2018 Mar]  
Culture condition
(Preculture condition)
Medium:  AF-6VTAC  
Temperature:  20 C
Light intensity:  20 µmol photons/m2/sec, L/D cycle:  10L:14D
Duration:  1 M  
Gene information ITS1-5.8S-ITS2 ( LC191309 ) , rbcL ( LC191317 ) , psbC ( LC191327 ) , act1 ( LC817975. )  
Cell size (min - max) 10 - 60 μm  
Organization Colonial; Flagellate 
Characteristics Other water bloom ; Phototaxis ; Endemic to Japan ; Homothallic ; Oogamy ; Haploid life cycle ; Resting zygote forming  
Other strain no. Other strain no. : 13-614-Vx13  
Remarks Fragile species to transportation stresses; Read and agree "How to order 4.1"; Axenic 
Movie  
Reference
Nozaki, H., Matsuzaki, R., Shimotori, K., Ueki, N., Heman, W., Mahakham, W., Yamaguchi, H., Tanabe, Y., Kawachi, M. 2024 Two species of the green algae Volvox sect. Volvox from the Japanese ancient lake, Lake Biwa. PLoS One, 19, e0310549 (article ID).
Strain(s): 543273627403874402140224258425943364337443446614662 
PubMed: 39312548
DOI: 10.1371/journal.pone.0310549

Nozaki, H., Ueki, N., Isaka, N., Saigo, T., Yamamoto, K., Matsuzaki, R., Takahashi, F., Wakabayashi, K-I., Kawachi, M. 2016 A new morphological type of Volvox from japanese large lakes and recent divergence of this type and V. ferrisii in two different freshwater habitats. PLoS One, 11, e0167148 (article ID).
Strain(s): 273627373987398840214022402340244025402640274028 
PubMed: 27880842
DOI: 10.1371/journal.pone.0167148

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