Strain number NIES-4017  
Phylum Chlorophyta  
Class Chlorophyceae  
Scientific name Astrephomene gubernaculifera Pocock  
Former name  
Common name Green alga  
Locality (Date of collection) Yokota, Sodegaura, Chiba, Japan (2014-07-21)  
Latitude / Longitude 35.389750 / 140.026722 
Habitat (Isolation source) Freshwater (Sediment)  
History < Yamashita, Shota; Nozaki, Hisayoshi  
Isolator (Date of isolation) Yamashita, Shota (2014-10-02)  
Identified by Yamashita, Shota  
State of strain Subculture; Unialgal; Clonal; Non-axenic[2018 Mar]  
Culture condition
(Preculture condition)
Medium:  VTAC  
Temperature:  20 C
Light intensity:  20 µmol photons/m2/sec, L/D cycle:  10L:14D
Duration:  14 D  
Gene information rbcL ( LC188997 )  
Cell size (min - max) 8 - 20 μm  
Organization Colonial; Flagellate 
Characteristics Mixotrophic ; Heterotrophic ; Phototaxis ; Heterothallic ; Isogamou ; Haploid life cycle ; Resting spore-forming  
Other strain no. Other strain no. : 2014-1002-YkAs8  
Yamashita, S., Arakaki, Y., Kawai-Toyooka, H., Noga, A., Hirono, M., Nozaki, H. 2016 Alternative evolution of a spheroidal colony in volvocine algae: developmental analysis of embryogenesis in Astrephomene (Volvocales, Chlorophyta). BMC Evol. Biol., 16, 243 (article ID).
Strain(s): 40174018 
PubMed: 27829356
DOI: 10.1186/s12862-016-0794-x

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