Strain number NIES-4016  
Phylum Chlorophyta  
Class Trebouxiophyceae  
Scientific name Nannochloris bacillaris Naumann  
Former name  
Common name Green alga  
Locality (Date of collection) Lake Nakatsuna, Nagano, Japan  
Latitude / Longitude 36.5978599855 / 137.843706252 
Habitat (Isolation source) Freshwater (Lake water)  
History < Yamamoto, Maki; Kawano, Shigeyuki < Kuroiwa, Tsuneyoshi < Ueda, Katsumi < Takamura, Noriko  
Isolator (Date of isolation) Takamura, Noriko  
Identified by Takamura, Noriko  
State of strain Cryopreservation; Unialgal; Non-clonal; Axenic[2018 Jan]  
Culture condition
(Preculture condition)
Medium:  C (agar)  
Temperature:  20 C
Light intensity:  15 µmol photons/m2/sec, L/D cycle:  10L:14D
Duration:  3 M  
Gene information 18S rRNA ( AB080300 ) , NbrbcS2, Akl, NbSep, PABP1 ( AB125314 ) , NbrbcSl-2, GAMMl, Mut2 ( AB125313 ) , RecG, NbrbcS1-1 ( AB125312 ) , NbrbcL ( AB383150 ) , NbftsZ2 ( AB119059 ) , nbact ( AB013098 )  
Cell size (min - max)  
Organization Unicellular 
Other strain no. Other strain no. : NT2'  
Remarks Cryopreserved; Axenic 
Yamamoto, M., Nozaki, H., Miyazawa, Y., Koide, T., Kawano, S. 2003 Relationship between presence of a mother cell wall and speciation in the unicellular microalga Nannochloris (Chlorophyta). J. Phycol., 39, 172-184.
Keywords: 18S rRNA; actin gene; autosporulation; binary fission; budding; Chlorella; Chlorophyta; molecular phylogeny; TBR, tree bisection reconnection
Strain(s): 21602169217321864016 
DOI: 10.1046/j.1529-8817.2003.02052.

Yamamoto, M., Nozaki, H., Kawano, S. 2001 Evolutionary relationships among multiple modes of cell division in the genus Nannochloris (Chlorophyta) revealed by genome size, actin gene multiplicity, and phylogeny. J. Phycol., 37, 106-120.
Keywords: actin gene; autosporulation; binary fission; budding; cell division; Chlorophyta; DNA content; genome size; phylogeny; Nannochloris
Strain(s): 21604016 
DOI: 10.1046/j.1529-8817.2001.037001106.x

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