Strain number NIES-4016  
Phylum Chlorophyta  
Class Trebouxiophyceae  
Scientific name Nannochloris bacillaris Naumann  
Former name  
Common name Green alga  
Locality (Date of collection) Lake Nakatsuna, Nagano, Japan  
Latitude / Longitude 36.5978599855 / 137.843706252 
Habitat (Isolation source) Freshwater (Lake water)  
History < Yamamoto, Maki; Kawano, Shigeyuki < Kuroiwa, Tsuneyoshi < Ueda, Katsumi < Takamura, Noriko  
Isolator (Date of isolation) Takamura, Noriko  
Identified by Takamura, Noriko  
State of strain Cryopreservation; Unialgal; Non-clonal; Axenic[2018 Jan]  
Culture condition
(Preculture condition)
Medium:  C (agar)  
Temperature:  20 C
Light intensity:  15 µmol photons/m2/sec, L/D cycle:  10L:14D
Duration:  3 M  
Gene information 18S rRNA ( AB080300 ) , NbrbcS2, Akl, NbSep, PABP1 ( AB125314 ) , NbrbcSl-2, GAMMl, Mut2 ( AB125313 ) , RecG, NbrbcS1-1 ( AB125312 ) , NbrbcL ( AB383150 ) , NbftsZ2 ( AB119059 ) , nbact ( AB013098 )  
Cell size (min - max)  
Organization Unicellular 
Other strain no. Other strain no. : NT2'  
Remarks Cryopreserved; Axenic 
Temraleeva, A., Krivina, E., Boldina, O. 2022 Edaphochloris, gen. nov.: a new genus of soil green algae (Trebouxiophyceae, Chlorophyta) with simple morphology. Plant Syst. Evol., 308, 4 (article ID).
Strain(s): 4016 
DOI: 10.1007/s00606-021-01795-8

Yamazaki, T ., Owari, S., Ota, S., Sumiya, N., Yamamoto, M., Watanabe, K., Nagumo, T. , Miyamura, S., Kawano, S. 2013 Localization and evolution of septins in algae. Plant J., 74, 605-614.
Keywords: green algae; Marvania geminata; Nannochloris bacillaris; algal septins; septin phylogeny
Strain(s): 4016 
PubMed: 23398289
DOI: 10.1111/tpj.1214

Sumiya, N., Yamazaki, T., Owari, S., Yamamoto, M., Watanabe, K., Kawano, S. 2012 Chloroplast division and differentially regulated expression of FtsZ1 and FtsZ2 in the synchronous culture of Nannochloris bacillaris (Chlorophyta, Trebouxiophyceae). J. Phycol., 77, 59-66.
Keywords: Chloroplast division; Nannochloris bacillaris; FtsZ ring formation; FtsZ1; FtsZ2
Strain(s): 4016 
DOI: 10.1508/cytologia.77.59

Sumiya, N. , Hirata, A., Kawano, S. 2008 Multiple FtsZ ring formation and reduplicated chloroplast DNA in Nannochloris bacillaris (Chlorophyta, Trebouxiophyceae) under phosphate-enriched culture. J. Phycol., 44, 1476-1489.
Keywords: chloroplast division; chloroplast DNA replication; FtsZ ring; Nannochloris bacillaris; phosphate-enriched culture
Strain(s): 4016 
DOI: 10.1111/j.1529-8817.2008.00589.x

Yamazaki, T., Yamamoto, M., Sakamoto, W., Kawano, S. 2005 Isolation and molecular characterization of rbcS the unicellular green alga Nannochloris bacillaris (Chlorophyta, Trebouxiophyceae). Phycol. Res., 53, 67-76.
Keywords: intron sliding; exon/intron structure; gene duplication; gene transfer; rbcS; ribulose-1, 5-bisphosphate carboxyase/oxygenase (Rubisco)
Strain(s): 4016 
DOI: 10.1111/j.1440-183.2005.00374.x

Yamamoto, M., Nozaki, H., Miyazawa, Y., Koide, T., Kawano, S. 2003 Relationship between presence of a mother cell wall and speciation in the unicellular microalga Nannochloris (Chlorophyta). J. Phycol., 39, 172-184.
Keywords: 18S rRNA; actin gene; autosporulation; binary fission; budding; Chlorella; Chlorophyta; molecular phylogeny; TBR, tree bisection reconnection
Strain(s): 21602169217321864016 
DOI: 10.1046/j.1529-8817.2003.02052.

Yamamoto, M., Nozaki, H., Kawano, S. 2001 Evolutionary relationships among multiple modes of cell division in the genus Nannochloris (Chlorophyta) revealed by genome size, actin gene multiplicity, and phylogeny. J. Phycol., 37, 106-120.
Keywords: actin gene; autosporulation; binary fission; budding; cell division; Chlorophyta; DNA content; genome size; phylogeny; Nannochloris
Strain(s): 21604016 
DOI: 10.1046/j.1529-8817.2001.037001106.x

Arai, S., Takahashi, H. , Takano, H. , Sakai, A., Kawano, S. 1998 Isolation, characterization, and chromosome mapping of an actin gene from the primitive green alga, Nannochloris bacillaris (Chlorophyceae). J. Phycol., 34, 477–485.
Keywords: actin gene; actin localization; cell division; karyotype; Nannochloris bacillaris
Strain(s): 4016 
PubMed: 8575024
DOI: 10.1046/j.1529-8817.1998.340477.x

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