Strain number NIES-4000  
Phylum Chlorophyta  
Class Chlorophyceae  
Scientific name Medakamo hakoo T.Kuroiwa  
Former name  
Common name Green alga  
Locality (Date of collection) Kagurazaka, Shinjuku-ku, Tokyo, Japan (2013-09-11)  
Latitude / Longitude 35.703 / 139.738 
Habitat (Isolation source) Freshwater (Moat water)  
History < Inui, Yayoi; Matsunaga, Sachihiro; Kuroiwa, Tsuneyoshi  
Isolator (Date of isolation) Inui, Yayoi; Matsunaga, Sachihiro (2015-10-20)  
Identified by Kuroiwa, Tsuneyoshi; Nozaki, Hisayoshi  
State of strain Cryopreservation; Unialgal; Clonal; Axenic  
Culture condition
(Preculture condition)
Medium:  C  
Temperature:  20 C
Light intensity:  6-10 µmol photons/m2/sec, L/D cycle:  10L:14D
Duration:  4 M  
Gene information Whole-genome ( CP089450-CP089465 ) , Mitochondrial DNA ( LC604817 ) , Plastid DNA ( LC604816 )  
Cell size (min - max) - 1 μm  
Organization Unicellular 
Characteristics Genome decoded strain (Kato et al. 2023) ; Type specimen (NIES-50025, Holotype)  
Other strain no. Other strain no. : Medakamo hakoo 311; M. hakoo 311  
Remarks Cryopreserved; Axenic 
Takusagawa, M., Misumi, O., Nozaki, H., Kato, S., Maruyama, S., Tsujimoto-Inui, Y., Yagisawa, F., Ohnuma, M., Kuroiwa, H, Kuroiwa, T., Matsunaga, S. 2024 Complete mitochondrial and chloroplast DNA sequences of the freshwater green microalga Medakamo hakoo. Genes Genet. Syst., 98, 353-360.
Keywords: mitochondria; chloroplast; organelle genome; Trebouxiophyceae
Strain(s): 4000 
PubMed: 38267054
DOI: 10.1266/ggs.23-00275

Kato, S., Misumi, O., Maruyama, S., Nozaki, H., Tsujimoto-Inui, Y., Takusagawa, M., Suzuki, S., Kuwata, Noda, S., Ito, N., Okabe, Y., Sakamoto, Yagisawa, Y., Matsunaga, M. T., Matsubayashi, Y., Yamaguchi, H., Kawachi, M., Kuroiwa, H., Kuroiwa, T., Matsunaga, S. 2023 Genomic analysis of an ultrasmall freshwater green alga, Medakamo hakoo. Commun. Biol., 6, 89 (article ID).
Strain(s): 4000 
PubMed: 36690657
DOI: 10.1038/s42003-022-04367-9

Kuroiwa, T., Ohnuma, M., Imoto, Y., Misumi, O., Nagata, N., Miyakawa, I., Fujishima, M., Yagisawa, F., Kuroiwa, H. 2016 Genome size of the ultrasmall unicellular freshwater green alga, Medakamo hakoo 311, as determined by staining with 4′,6-diamidino-2-phenylindole after microwave oven treatments: II. Comparison with Cyanidioschyzon merolae, Saccharomyces cerevisiae (n, 2n), and Chlorella variabilis. Cytologia, 81, 69-76.
Keywords: Ultrasmall genome size; Medakamo hakoo; Saccharyomyces cerevisiae; Chlorella variabilis; DAPI staining; Microwave oven
Strain(s): 33774000 
DOI: 10.1508/cytologia.81.69

Kuroiwa, T., Ohnuma, M., Nozaki, H., Imoto, Y., Misumi, O., Kuroiwa, H. 2015 Cytological evidence of cell-nuclear genome size of a new ultra-small unicellular freshwater green alga, “Medakamo hakoo” strain M-hakoo 311 I. Comparison with Cyanidioschyzon merolae and Ostreococcus tauri. Cytologia, 80, 143-150.
Keywords: Ultra-small alga; Chlorophyte M. hakoo; Cyanidioschyzon merolae; Ostreococcus tauri; Genome size; Cytochemistry
Strain(s): 267333774000 
DOI: 10.1508/cytologia.80.143

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