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Strain Data
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| Strain number | NIES-4000 | |||
|---|---|---|---|---|
| Phylum | Chlorophyta | |||
| Class | Chlorophyceae | |||
| Scientific name | Medakamo hakoo T.Kuroiwa | |||
| Synonym | ||||
| Former name | ||||
| Common name | Green alga | |||
| Locality (Date of collection) | Kagurazaka, Shinjuku-ku, Tokyo, Japan (2013-09-11) | |||
| Latitude / Longitude | 35.703 / 139.738 | |||
| Habitat (Isolation source) | Freshwater (Moat water) | |||
| History | < Inui, Yayoi; Matsunaga, Sachihiro; Kuroiwa, Tsuneyoshi | |||
| Isolator (Date of isolation) | Inui, Yayoi; Matsunaga, Sachihiro (2015-10-20) | |||
| Identified by | Kuroiwa, Tsuneyoshi; Nozaki, Hisayoshi | |||
| State of strain | Cryopreservation; Unialgal; Clonal; Axenic | |||
|
Culture condition (Preculture condition) |
Medium:
C
Temperature: 20 C Light intensity: 6-10 µmol photons/m2/sec, L/D cycle: 10L:14D Duration: 4 M |
|||
| Gene information | Whole-genome ( CP089450-CP089465 ) , Mitochondrial DNA ( LC604817 ) , Plastid DNA ( LC604816 ) | |||
| Cell size (min - max) | - 1 μm | |||
| Organization | Unicellular | |||
| Characteristics | Genome decoded strain (Kato et al. 2023) ; Type specimen (NIES-50025, Holotype) | |||
| Other strain no. | Other strain no. : Medakamo hakoo 311; M. hakoo 311 | |||
| Remarks | Cryopreserved; Axenic | |||
| Movie | ||||
| Reference |
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Takusagawa, M., Misumi, O., Nozaki, H., Kato, S., Maruyama, S., Tsujimoto-Inui, Y., Yagisawa, F., Ohnuma, M., Kuroiwa, H, Kuroiwa, T., Matsunaga, S.
2024
Complete mitochondrial and chloroplast DNA sequences of the freshwater green microalga Medakamo hakoo.
Genes Genet. Syst.,
98,
353-360.
Keywords: mitochondria; chloroplast; organelle genome; Trebouxiophyceae Strain(s): 4000 PubMed: 38267054 DOI: 10.1266/ggs.23-00275 Kato, S., Misumi, O., Maruyama, S., Nozaki, H., Tsujimoto-Inui, Y., Takusagawa, M., Suzuki, S., Kuwata, Noda, S., Ito, N., Okabe, Y., Sakamoto, Yagisawa, Y., Matsunaga, M. T., Matsubayashi, Y., Yamaguchi, H., Kawachi, M., Kuroiwa, H., Kuroiwa, T., Matsunaga, S. 2023 Genomic analysis of an ultrasmall freshwater green alga, Medakamo hakoo. Commun. Biol., 6, 89 (article ID). Strain(s): 4000 PubMed: 36690657 DOI: 10.1038/s42003-022-04367-9 Kuroiwa, T., Ohnuma, M., Imoto, Y., Misumi, O., Nagata, N., Miyakawa, I., Fujishima, M., Yagisawa, F., Kuroiwa, H. 2016 Genome size of the ultrasmall unicellular freshwater green alga, Medakamo hakoo 311, as determined by staining with 4′,6-diamidino-2-phenylindole after microwave oven treatments: II. Comparison with Cyanidioschyzon merolae, Saccharomyces cerevisiae (n, 2n), and Chlorella variabilis. Cytologia, 81, 69-76. Keywords: Ultrasmall genome size; Medakamo hakoo; Saccharyomyces cerevisiae; Chlorella variabilis; DAPI staining; Microwave oven Strain(s): 3377, 4000 DOI: 10.1508/cytologia.81.69 Kuroiwa, T., Ohnuma, M., Nozaki, H., Imoto, Y., Misumi, O., Kuroiwa, H. 2015 Cytological evidence of cell-nuclear genome size of a new ultra-small unicellular freshwater green alga, “Medakamo hakoo” strain M-hakoo 311 I. Comparison with Cyanidioschyzon merolae and Ostreococcus tauri. Cytologia, 80, 143-150. Keywords: Ultra-small alga; Chlorophyte M. hakoo; Cyanidioschyzon merolae; Ostreococcus tauri; Genome size; Cytochemistry Strain(s): 2673, 3377, 4000 DOI: 10.1508/cytologia.80.143 |
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