Strain number NIES-3783  
Phylum Chlorophyta  
Class Chlorophyceae  
Scientific name Volvox reticuliferus Nozaki  
Synonym  
Former name  
Common name Green alga ; Volvocine alga  
Locality (Date of collection) Lake Biwa, Oroshimo-cho, Kusatsu Shiga, Japan (2013-07-03)  
Latitude / Longitude 35.073889 / 135.931944 
Habitat (Isolation source) Freshwater (Lake water)  
History < Nozaki, Hisayoshi  
Isolator (Date of isolation) Nozaki, Hisayoshi (2013-07-03)  
Identified by Nozaki, Hisayoshi  
State of strain Subculture; Unialgal; Clonal; Axenic[2022 Apr]  
Culture condition
(Preculture condition)
Medium:  AF-6/2AF-6/3  
Temperature:  20 C
Light intensity:  12-23 µmol photons/m2/sec, L/D cycle:  10L:14D
Duration:  1 M  
Gene information ITS2 ( LC090162 )  
Cell size (min - max)  
Organization Colonial; Flagellate 
Characteristics Phototaxis ; Heterothallic ; Dioecious ; Oogamy ; Male ; F1 strains (NIES-3785, 3786)  
Other strain no. Other strain no. : 2013-0703-VO3  
Remarks Axenic; Fragile species to transportation stresses; Read and agree "How to order 4.1" 
Movie  
Reference
Nozaki, H., Mahakham, W., Heman W., Matsuzaki, R., Kawachi, M. 2022 Morphology, mating system and taxonomy of Volvox africanus (Volvocaceae, Chlorophyceae) from Thailand. Bot. Stud., 63, 1 (article ID).
Strain(s): 378037813782378344674468 
PubMed: 35061120
DOI: 10.1186/s40529-022-00332-1

Yamamoto, K., Hamaji, T., Kawai-Toyooka, H., Matsuzaki, R., Takahashi, F., Nishimura, Y., Kawachi, M., Noguchi, H., Minakuchi, YY., Umen, J. G., Toyoda, A., Nozaki, H. 2021 Three genomes in the algal genus Volvox reveal the fate of a haploid sex-determining region after a transition to homothallism. Proc. Natl. Acad. Sci. U. S. A., 118, e2100712118 (article ID).
Keywords: Volvox; evolution; heterothallism; homothallism; sex
Strain(s): 3780378137823783378437853786 
PubMed: 34011609
DOI: 10.1073/pnas.2100712118

Yamamoto, K., Kawai-Toyooka, H., Hamaji, T., Tsuchikane, Y., Mori, T., Takahashi, F., Sekimoto, H., Ferris, P. J., Nozaki, H. 2017 Molecular evolutionary analysis of a gender-limited MID ortholog from the homothallic species Volvox africanus with male and monoecious spheroids. PLoS One, 12, e0180313 (article ID).
Strain(s): 37803782378337853786398641104111 
PubMed: 28665990
DOI: 10.1371/journal.pone.0180313

Nozaki, H., Matsuzaki, R., Yamamoto, K., Kawachi, M., Takahashi, F. 2015 Delineating a new heterothallic species of Volvox (Volvocaceae, Chlorophyceae) using new strains of “Volvox africanus”. PLoS One, 10, e0142632 (article ID).
Strain(s): 8633780378137823783378437853786 
PubMed: 26562165
DOI: 10.1371/journal.pone.0142632

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