Strain number NIES-3769  
Phylum Chlorophyta  
Class Chlorophyceae  
Scientific name Pyrobotrys casinoensis (Playfair) P.C.Silva  
Synonym Uva casinoensis Playfair 1914; Chlamydobotrys gracilis Korshikov 1924; Chlorobrachis gracillima Korshikov 1925; Pyrobotrys gracilis (Korshikov) Korshikov 1938  
Former name  
Common name Green alga  
Locality (Date of collection) Oxidation Pond, Stechlin, Oberhavel, Brandenburg, Germany (2013-09-29)  
Latitude / Longitude 53.148306 / 13.053667 
Habitat (Isolation source) Freshwater (Sediment)  
History < Krienitz, Lother; Sugasawa, Mizuho  
Isolator (Date of isolation) Krienitz, Lother; Sugasawa, Mizuho (2014-03-13)  
Identified by Sugasawa, Mizuho; Nozaki, Hisayoshi  
State of strain Subculture; Unialgal; Clonal; Axenic[2018 Mar]  
Culture condition
(Preculture condition)
Medium:  AF-6  
Temperature:  20 C
Light intensity:  21 µmol photons/m2/sec, L/D cycle:  10L:14D
Duration:  1 M  
Gene information  
Cell size (min - max)  
Organization Colonial; Flagellate 
Characteristics Mixotrophic ; Osmotrophic ; Phototaxis ; Microaerophilic  
Other strain no. Other strain no. : DP21  
Remarks Fragile species to transportation stresses; Read and agree "How to order 4.1"; Axenic 
Sugasawa, M., Matsuzaki, R., Kawafune, K., Takahashi, T., Kawachi, M., Krientiz, L., Nozaki, H. 2015 Taxonomic study of Pyrobotrys (Spondylomoraceae, Chlorophyceae) based on comparative morphological and molecular analyses of culture strains established using novel methods. Cytologia, 80, 513-524.
Keywords: Molecular phylogeny; Morphology; Pyrobotrys; Pyrobotrys elongata; Spondylomoraceae; Stigma; Taxonomy; Ultrastructure
Strain(s): 3768376937703771377237733774 
DOI: 10.1508/cytologia.80.513

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