Strain Data

Strain number		NIES-3754
Phylum		Cyanophyta
Class		Cyanophyceae
Scientific name		Mastigocladus sp.
Synonym
Former name		Formerly identified as Fischerella sp., named based on Casamatta et al. 2020[2020 Mar]
Common name		Blue-green alga ; Cyanobacteria
Locality (Date of collection)		Suwa Shrine, Nagano, Japan (2013-11-01)
Latitude / Longitude		36.075287 / 138.090472
Habitat (Isolation source)		Hot spring (Water)
History		< Hirose, Yuu
Isolator (Date of isolation)		Hirose, Yuu (2013-11-01)
Identified by		Hirose, Yuu
State of strain		Cryopreservation; Unialgal; Clonal; Axenic
Culture condition (Preculture condition)		Medium: BG-11 Temperature: 37-45 C Light intensity: 9-10 µmol photons/m²/sec, L/D cycle: 10L:14D Duration: 2 M
Gene information		Whole-genome ( NZ_AP017305 ) , Plasmid DNA ( NZ_AP017306 NZ_AP017307 )
Cell size (min - max)
Organization
Characteristics		Genome decoded strain (Hirose et al. 2016)
Other strain no.		Other strain no. : SW1
Remarks		Cryopreserved; Axenic
Movie

Reference
Nowruzi, B., Hutarova, L., Vešelenyiova, D., Metcalf, J. S. 2024 Characterization of Neowestiellopsis persica A1387 (Hapalosiphonaceae) based on the cpcA, psbA, rpoC1, nifH and nifD gene sequences. BMC Ecol. Evol., 24, 57 (article ID). Keywords: Cyanobacteria; Phylogenetic analysis; Neowestiellopsis persica, cpcA Strain(s): 21, 22, 23, 25, 50, 73, 267, 592, 2098, 2100, 2107, 3275, 3754, 3756, 3974, 4073, 4074, 4101, 4103, 4106 PubMed: 38711016 DOI: 10.1186/s12862-024-02244-z Giordano, M., Goodman, C. A., Huang, F., Raven, J. A., Ruan, Z. 2022 A mechanistic study of the influence of nitrogen and energy availability on the NH₄⁺ sensitivity of nitrogen assimilation in Synechococcus. J. Exp. Bot., 73, 5596-5611. Keywords: Ammonium; cyanobacteria; glutamine synthetase; limitation; nitrate reductase; nitrite reductase; NtcA regulation; N metabolism Strain(s): 25, 50, 204, 267, 843, 970, 981, 2107, 2134, 2135, 2481, 2549, 3709, 3754, 3756, 3757, 4074 PubMed: 35595516 DOI: 10.1093/jxb/erac219 Jaiswal, T. P., Chakraborty, S., Singh, P., Mishra, A. K., Singh, S. S. 2021 Description of hot spring dwelling Mastigocladus ambikapurensis sp. nov., using a polyphasic approach. Plant Syst. Evol., 307, 33 (article ID). Keywords: 16S rRNA gene; Cyanobacteria; ITS; Mastigocladus; Phylogeny Strain(s): 592, 3754 DOI: 10.1007/s00606-021-01755-2 Jung, P., D'Agostino, P. M., Brust, K., Büdel, B., Lakatos, M. 2021 Final destination? Pinpointing Hyella disjuncta sp. nov. PCC 6712 (Cyanobacteria) based on taxonomic aspects, multicellularity, nitrogen fixation and biosynthetic gene clusters. Life-Basel, 11, 916 (article ID). Strain(s): 592, 3754 PubMed: 34575065 DOI: 10.3390/life11090916 Casamatta, D. A., Villanueva, C. D., Garvey, A. D., Stocks, H. S., Vaccarino, M., Dvořák, P., Hašler, P., Johansen, J. R. 2020 Reptodigitus chapmanii (Nostocales, Hapalosiphonaceae) gen. nov.: a unique Nostocalean (Cyanobacteria) genus based on a polyphasic approach. J. Phycol., 56, 425-436. Keywords: cyanobacteria; Phylogeny; 16S rDNA; systematics Strain(s): 592, 3754, 4106 PubMed: 31837017 DOI: 10.1111/jpy.12954 MacCready, J. S., Basalla, J. L., Vecchiarelli, A. G. 2020 Origin and evolution of carboxysome positioning systems in cyanobacteria. Mol. Biol. Evol., 37, 1434–1451. Strain(s): 21, 22, 25, 26, 30, 39, 44, 73, 87, 88, 267, 592, 806, 843, 932, 970, 2098, 2100, 2107, 2108, 2111, 2549, 3275, 3754, 3756, 3974, 4073, 4074, 4075, 4101, 4103, 4106 PubMed: 31899489 DOI: 10.1093/molbev/msz308 Alcorta, J., Vergara-Barros, P., Antonaru, L. A., Alcamán-Arias, M. E., Nürnberg, D. J., Díez, B. 2019 Fischerella thermalis: a model organism to study thermophilic diazotrophy, photosynthesis and multicellularity in cyanobacteria. Extremophiles, 23, 635–647. Keywords: Fischerella: Mastigocladus: Distribution: Thermophile: Nitrogen fxation: Photosynthesis: Multicellularity: Hot springs Strain(s): 592, 3754, 4106 PubMed: 31512055 DOI: 10.1007/s00792-019-01125-4 Moten, D., Batsalova, T., Basheva, D., Mladenov, R., Dzhambazov, B., Teneva, I. 2018 Outer membrane efflux protein (OMEP) is a suitable molecular marker for resolving the phylogeny and taxonomic status of closely related cyanobacteria. Phycol. Res., 66, 31-36. Keywords: cyanobacterial taxonomy; phylogenetic analysis; transmembrane transporter sequences Strain(s): 39, 843, 2104, 3754, 3755, 3756, 3757 DOI: 10.1111/pre.12203 Antonaru, L. A., Nurnberg, D. J. 2017 Role of PatS and cell type on the heterocyst spacing pattern in a filamentous branching cyanobacterium. FEMS Mircobiol. Lett., 364 fnx154 (article ID). Keywords: multicellularity; cyanobacteria; Stigonematales; cell differentiation; nitrogen fixation; heterocyst spacing pattern; PatS Strain(s): 592, 3754, 3756 PubMed: 28859320 DOI: 10.1093/femsle/fnx154 Hirose, Y., Fujisawa, T., Ohtsubo, Y., Katayama, M., Misawa, N., Wakazuki, S., Shimura, Y., Nakamura, Y., Kawachi, M., Yoshikawa, H., Eki, T., Kanesaki, Y. 2016 Complete genome sequence of cyanobacterium Fischerella sp. NIES-3754, providing thermoresistant optogenetic tools. J. Biotechnol., 220, 45-46. Keywords: Cyanobacteria; Genome; Photosensor; Thermophile Strain(s): 3754 PubMed: 26784989 DOI: 10.1016/j.jbiotec.2016.01.011

Reference

Nowruzi, B., Hutarova, L., Vešelenyiova, D., Metcalf, J. S. 2024 Characterization of Neowestiellopsis persica A1387 (Hapalosiphonaceae) based on the cpcA, psbA, rpoC1, nifH and nifD gene sequences. BMC Ecol. Evol., 24, 57 (article ID).
Keywords: Cyanobacteria; Phylogenetic analysis; Neowestiellopsis persica, cpcA
Strain(s): 21, 22, 23, 25, 50, 73, 267, 592, 2098, 2100, 2107, 3275, 3754, 3756, 3974, 4073, 4074, 4101, 4103, 4106
PubMed: 38711016
DOI: 10.1186/s12862-024-02244-z

Giordano, M., Goodman, C. A., Huang, F., Raven, J. A., Ruan, Z. 2022 A mechanistic study of the influence of nitrogen and energy availability on the NH₄⁺ sensitivity of nitrogen assimilation in Synechococcus. J. Exp. Bot., 73, 5596-5611.
Keywords: Ammonium; cyanobacteria; glutamine synthetase; limitation; nitrate reductase; nitrite reductase; NtcA regulation; N metabolism
Strain(s): 25, 50, 204, 267, 843, 970, 981, 2107, 2134, 2135, 2481, 2549, 3709, 3754, 3756, 3757, 4074
PubMed: 35595516
DOI: 10.1093/jxb/erac219

Jaiswal, T. P., Chakraborty, S., Singh, P., Mishra, A. K., Singh, S. S. 2021 Description of hot spring dwelling Mastigocladus ambikapurensis sp. nov., using a polyphasic approach. Plant Syst. Evol., 307, 33 (article ID).
Keywords: 16S rRNA gene; Cyanobacteria; ITS; Mastigocladus; Phylogeny
Strain(s): 592, 3754
DOI: 10.1007/s00606-021-01755-2

Jung, P., D'Agostino, P. M., Brust, K., Büdel, B., Lakatos, M. 2021 Final destination? Pinpointing Hyella disjuncta sp. nov. PCC 6712 (Cyanobacteria) based on taxonomic aspects, multicellularity, nitrogen fixation and biosynthetic gene clusters. Life-Basel, 11, 916 (article ID).
Strain(s): 592, 3754
PubMed: 34575065
DOI: 10.3390/life11090916

Casamatta, D. A., Villanueva, C. D., Garvey, A. D., Stocks, H. S., Vaccarino, M., Dvořák, P., Hašler, P., Johansen, J. R. 2020 Reptodigitus chapmanii (Nostocales, Hapalosiphonaceae) gen. nov.: a unique Nostocalean (Cyanobacteria) genus based on a polyphasic approach. J. Phycol., 56, 425-436.
Keywords: cyanobacteria; Phylogeny; 16S rDNA; systematics
Strain(s): 592, 3754, 4106
PubMed: 31837017
DOI: 10.1111/jpy.12954

MacCready, J. S., Basalla, J. L., Vecchiarelli, A. G. 2020 Origin and evolution of carboxysome positioning systems in cyanobacteria. Mol. Biol. Evol., 37, 1434–1451.
Strain(s): 21, 22, 25, 26, 30, 39, 44, 73, 87, 88, 267, 592, 806, 843, 932, 970, 2098, 2100, 2107, 2108, 2111, 2549, 3275, 3754, 3756, 3974, 4073, 4074, 4075, 4101, 4103, 4106
PubMed: 31899489
DOI: 10.1093/molbev/msz308

Alcorta, J., Vergara-Barros, P., Antonaru, L. A., Alcamán-Arias, M. E., Nürnberg, D. J., Díez, B. 2019 Fischerella thermalis: a model organism to study thermophilic diazotrophy, photosynthesis and multicellularity in cyanobacteria. Extremophiles, 23, 635–647.
Keywords: Fischerella: Mastigocladus: Distribution: Thermophile: Nitrogen fxation: Photosynthesis: Multicellularity: Hot springs
Strain(s): 592, 3754, 4106
PubMed: 31512055
DOI: 10.1007/s00792-019-01125-4

Moten, D., Batsalova, T., Basheva, D., Mladenov, R., Dzhambazov, B., Teneva, I. 2018 Outer membrane efflux protein (OMEP) is a suitable molecular marker for resolving the phylogeny and taxonomic status of closely related cyanobacteria. Phycol. Res., 66, 31-36.
Keywords: cyanobacterial taxonomy; phylogenetic analysis; transmembrane transporter sequences
Strain(s): 39, 843, 2104, 3754, 3755, 3756, 3757
DOI: 10.1111/pre.12203

Antonaru, L. A., Nurnberg, D. J. 2017 Role of PatS and cell type on the heterocyst spacing pattern in a filamentous branching cyanobacterium. FEMS Mircobiol. Lett., 364 fnx154 (article ID).
Keywords: multicellularity; cyanobacteria; Stigonematales; cell differentiation; nitrogen fixation; heterocyst spacing pattern; PatS
Strain(s): 592, 3754, 3756
PubMed: 28859320
DOI: 10.1093/femsle/fnx154

Hirose, Y., Fujisawa, T., Ohtsubo, Y., Katayama, M., Misawa, N., Wakazuki, S., Shimura, Y., Nakamura, Y., Kawachi, M., Yoshikawa, H., Eki, T., Kanesaki, Y. 2016 Complete genome sequence of cyanobacterium Fischerella sp. NIES-3754, providing thermoresistant optogenetic tools. J. Biotechnol., 220, 45-46.
Keywords: Cyanobacteria; Genome; Photosensor; Thermophile
Strain(s): 3754
PubMed: 26784989
DOI: 10.1016/j.jbiotec.2016.01.011

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