Strain number NIES-3752  
Phylum Chlorophyta  
Class Chlorophyceae  
Scientific name Chloromonas krienitzii Matsuzaki & Nozaki  
Synonym  
Former name  
Common name Green alga ; Snow alga  
Locality (Date of collection) Yamagata Prefectural Nature Conservation Park, Mt. Gassan, Nishikawa, Yamagata, Japan (2013-06-30)  
Latitude / Longitude 38.513889 / 139.990833 
Habitat (Isolation source) Terrestrial (Snow)  
History < Matsuzaki, Ryo  
Isolator (Date of isolation) Matsuzaki, Ryo (2014-01-24)  
Identified by Matsuzaki, Ryo; Nozaki, Hisayoshi  
State of strain Subculture; Unialgal; Clonal; Non-axenic  
Culture condition
(Preculture condition)
Medium:  AF-6  
Temperature:  5 C
Light intensity:  36 µmol photons/m2/sec, L/D cycle:  10L:14D
Duration:  3 M  
Gene information 18S rRNA ( LC012713 ) , 26S rRNA ( LC060475 ) , ITS2 ( LC012757 ) , atpB ( LC012721 ) , rbcL ( LC012741 ) , psaB ( LC012729 )  
Cell size (min - max)  
Organization Unicellular; Flagellate 
Characteristics Cryophilic  
Other strain no. Other strain no. : GsCl-49  
Remarks Fragile species to temperature changes 
Movie  
Reference
Matsuzaki, R., Kawai-Toyooka, H., Hara, Y., Nozaki, H. 2015 Revisiting the taxonomic significance of aplanozygote morphologies of two “cosmopolitan” snow species of the genus Chloromonas (Volvocales, Chlorophyceae). Phycologia, 54, 491–502.
Keywords: Chloromonas brevispina; Chloromonas krienitzii sp. nov.; Chloromonas nivalis; Molecular phylogeny; Morphology; Snow algae; Ultrastructure
Strain(s): 23792380338937523753 
DOI: 10.2216/15-33.1

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