A big image opens when the thumbnail is clicked.(nies-0226.jpg)
Strain number NIES-226  
Phylum Chlorophyta  
Class Chlorophyceae  
Scientific name Graesiella emersonii (Shihira & Krauss) Nozaki et al.  
Synonym Chlorella emersonii Shihira & Krauss; Chlorella fusca Shihira & Krauss var. vacuolata Shihira & Krauss; Graesiella vacuolata (Shihira & Krauss) Kalina & Puncochárová  
Former name Chlorella pyrenoidosa Chick  
Common name Green alga  
Locality (Date of collection) Japan  
Latitude / Longitude  
Habitat (Isolation source)  
History < IAM (1983);  
Isolator (Date of isolation)  
Identified by Nozaki, Hisayoshi et al. (Reidentify)  
State of strain Cryopreservation; Unialgal; Clonal; Axenic[2014 Feb]  
Culture condition
(Preculture condition)
Medium:  C (agar)  
Temperature:  20 (25) C
Light intensity:  4-10 (14-16) µmol photons/m2/sec, L/D cycle:  10L:14D
Duration:  3 M (14 D)  
Gene information  
Cell size (min - max) 5 - 9 μm  
Organization Unicellular 
Other strain no. Other collection strain no. : IAM C-28
Remarks Cryopreserved; Axenic 
Hamana, K., Furuchi, T., Hayashi, H., Uemura, T., Niitsu, M. 2023 Additional polyamine analysis of the algae belonging to the phyla Glaucophyta, Rhodophyta and Chlorophyta. ─Polyamine analysis of algae V─ Microb. Resour. Syst., 39, 33-40.
Keywords: alga; Chlorophyta; Glaucophyta; polyamine; Rhodophyta
Strain(s): 22625025455055162612981332170318362137216321642165216622522353289433773645386738913892410743904452 

Tawfik, A., Niaz, H., Qadeer, K., Qyyum, M. A., Liu, J. J., Lee, M. 2022 Valorization of algal cells for biomass and bioenergy production from wastewater: Sustainable strategies, challenges, and techno-economic limitations. Renew. Sust. Energ. Rev., 157, 112024 (article ID).
Keywords: Xenobiotic compounds; Micro-pollutants; Enzyme activity; Biomass; Bioenergy; Algae-based wastewater treatment
Strain(s): 192344226422437216922203284 
DOI: 10.1016/j.rser.2021.112024

Hamasaki, A. 2017 Study of the optimal cultivation for micro-algae. The 12th Conference on Biomass Science, 27-28 (in Japanese with English summary).
Strain(s): 226 
DOI: 10.20550/jiebiomassronbun.12.0_27

Chu, C. Y., Liao, W. R., Huang, R., Lin, L. P. 2004 Haemagglutinating and antibiotic activities of freshwater microalgae. World J. Microbiol. Biotechnol., 20, 817–825.
Keywords: Antibiotic activity; erythrocytes; freshwater microalgae; haemagglutination
Strain(s): 22622721592163216521692175218922082211221322142229223122332240224122432247224832773284 
DOI: 10.1007/s11274-004-8712-6

Mori, F., Erata, M., Watanabe, M. M. 2002 Cryopreservation of cyanobacteria and green algae in the NIES-Collection. Microbiol. Cult. Coll., 18, 45-55.
Keywords: cryopreservation; cyanobacteria; green algae; FDA staining method; NIES-Collection
Strain(s): 192122232425262728293031323334353637383940414445465051525354555658596061626364656667686970737475767778798081878990919293949596979899100101102103104105106107108109110111112119120122123125127128129130131132133134135137138139144147151152153154155156157158159160162163164165166167168170171172173174175176177178179180181182183185186187188189191192193194197198199200201202203204205206207208209210211212213214215216217224226227228229230231232241242243244245246248257259261262263266267268287288289290294295297298299300301302303305306308309310312313329334337338339340341342349351359360361362375378379380382384385390394396397398415416418419421422423424425426427428429430431432433434436437438439440446447448449450451452453454455456457458459460464468469474478479480481503504505506507509510512514515522523524527528529530531532536537538539540541542543544545546564565566567568569570571572573574575576577578579580581582583584585592593594595596597598604610628630631632633634635636637638639640641642643644645646647648649650651652653654655656657658659660661662663664665666667672685686687688689690691692693694717718719720721722723724725726727728729 

Kuramoto, T., Iwamoto, K., Izumi, M., Kirihata, M., Yoshizako, F. 1999 Asymmetric reduction of ethyl 2-methyl 3-oxobutanoate by Chlorella. Biosci. Biotechnol. Biochem., 63, 598-601.
Keywords: Chlorella; asymmetric reduction; ethyl 2-methyl 3-oxobutanoate; (2S, 3S)-ethyl 2-methyl 3-hydroxybutanoate; (2S, 3R)-ethyl 2-methyl 3-hydroxybutanoate
Strain(s): 226227 
PubMed: 10227152
DOI: 10.1271/bbb.63.598

Nozaki, H., Katagiri, M., Nakagawa, M., Aizawa, K., Watanabe, M. M. 1995 Taxonomic re-examination of the two strains labeled Chlorella in the Microbial Culture Collection at the National Institute for Environmental Studies (NIES-Collection). Microbiol. Cult. Coll., 11, 11-18.
Keywords: Chlorella; Graesiella emersonii comb. nov.; green algae; morphology; taxonomy
Strain(s): 226227686687688689690 

Yoshizako, F., Ogino, M., Nishimura, A., Chubachi, M., Horii, T. 1995 Biotransformation of cyclic β-keto esters by Chlorella pyrenoidosa Chick. J. Ferment. Bioeng., 79, 141-145.
Keywords: Chlorella pyrenoidosa Chick; cyclic β-keto esters; biotransformation; alkoxycarbolyl group elimination; carbonyl group reduction
Strain(s): 226 
DOI: 10.1016/0922-338X(95)94081-2

Yoshizako, F., Nishimura, A., Chubachi, M. 1994 Identification of algal transformation products from alicyclic ketones. J. Ferment. Bioeng., 77, 144-147.
Strain(s): 226 
DOI: 10.1016/0922-338X(94)90313-1

Watanabe, Y., Ohmura, N., Saiki, H. 1992 Microbial CO2 fixation. 2. Isolation and determination of cultural characteristics of Chlorella strains which function under CO2 enriched atmosphere. CRIEPI Kenkyû-Hôkoku, U92014, 1-21 (in Japanese with English summary).
Keywords: climate change; CO2; CO2 fixation; microalgae; Chlorella
Strain(s): 27459294122158204226227 

Yoshizako, F., Nishimura, A., Chubachi, M. 1992 Microbial reduction of cyclohexanone by Chlorella pyrenoidosa Chick. J. Ferment. Bioeng., 74, 395-397.
Strain(s): 226 
DOI: 10.1016/0922-338X(92)90039-W

Yoshizako, F., Nishimura, A., Chubachi, M., Horii, T., Ueno, T. 1991 Bioconversion of cyclohexaneacetic acid to monohydroxycyclo-hexaneacetic acids by Chlorella pyrenoidosa Chick. J. Ferment. Bioeng., 72, 343-346.
Strain(s): 226227 

Maruyama, I., Ando, Y., Maeda, T., Hirayama, K. 1989 Uptake of vitamin B12 by various strains of unicellular algae Chlorella. Nippon Suisan Gakkaishi, 55, 1785-1790.
Strain(s): 48226687215721692175 

Takeda, H. 1988 Classification of Chlorella strains by cell wall sugar composition. Phytochemistry, 27, 3823-3826.
Keywords: Chlorella ellipsoidea; C. fusca; C. kessleri; C. saccharophila; C. sorokiniana; C. vulgaris; Chlorophyta; anisotropy; cell wall sugar composition; chemotaxonomy; Ruthenium Red staining
Strain(s): 226215021602170 
DOI: 10.1016/0031-9422(88)83025-5

Hirokawa, T. & Takeda, H. 1980 Automatic analysing system for the liquid chromatography. J. Gen. Edu. Dept., Niigata Univ., 10, 97-106.
Strain(s): 2262170 

Maruyama, K. 1977 Classification of Chlorella strain by infrared absorption spectra of cell samples. Bot. Mag. Tokyo, 90, 67-77.
Strain(s): 2262276856872151216321702175 
DOI: 10.1007/BF02489470

Maruyama, K. 1977 Classification of Chlorella strains by cell appearance and group sera. Bot. Mag. Tokyo, 90, 57-66.
Strain(s): 2262276852151216321702175 
DOI: 10.1007/BF02489469

Ichimura, T. & Itoh, T. 1977 17. Preservation methods of microalgae (I) [17. Bisaisôrui no hozonhô (I)]. In Preservation methods of microorganisms [Biseibutsu Hozonhô], Ed. by Nei, T., University of Tokyo Press, Tokyo, pp. 355-373 (in Japanese).
Strain(s): 19212223242728303132333436373839404144454647484950122123127131144152173175179180181182183185198200203206217226227241266267268295309310334338379436448450455527 

Mihara, S. & Hase, E. 1976 A short note on the effects of 6-methyl purine on the revolution of the cell cycle of Chlorella pyrenoidosa in synchronous culture. Plant Cell Physiol., 17, 403-406.
Strain(s): 226 
DOI: 10.1093/oxfordjournals.pcp.a075292

Naohara, T. & Ishikawa, H. 1975 Assay of the activity of herbicides using Chlorella. Zassô-Kenkyû, 20, 82-86 (in Japanese with English summary).
Strain(s): 226 

Watanabe, A. 1960 List of algal strains in collection at the Institute of Applied Microbiology, University of Tokyo. J. Gen. Appl. Microbiol., 6, 283-292.
Strain(s): 192122232425262829374749226227 

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