Strain number NIES-3744  
Phylum Chlorophyta  
Class Chlorophyceae  
Scientific name Dunaliella sp.  
Former name  
Common name Green alga  
Locality (Date of collection) Lake Funazoko-ike Skarvsnes Antarctica (1975-01-25)  
Latitude / Longitude / 39.55 
Habitat (Isolation source) Brackish water (Lake water)  
History < Kikuchi, Mikio < Watanuki, Tomohiko  
Isolator (Date of isolation) Watanuki, Tomohiko (1987-07-14)  
Identified by Watanuki, Tomohiko  
State of strain Subculture; Unialgal; Non-clonal; Non-axenic  
Culture condition
(Preculture condition)
Medium:  Modified Johnson's medium  
Temperature:  20 C
Light intensity:  25 µmol photons/m2/sec, L/D cycle:  10L:14D
Duration:  3 M  
Gene information  
Cell size (min - max)  
Organization Unicellular; Flagellate 
Characteristics Water bloom ; Halophilic  
Other strain no. Other strain no. : hunazoko  
Tsutsumi, N., Kikuchi, M., Sawai, J. 2015 Development of algal growth inhibition test with saltwater alga Dunaliella sp. : Toxicity of 39 chemicals. Jpn. J. Environ. Toxicol., 18, 73-83 (in Japanese with English summary).
Keywords: saltwater alga; Dunaliella; herbicide; toxicity; log Pow, susceptibility
Strain(s): 3744 

Mihirogi, M., Kikuchi, M., Sawai, J. 2012 Development of screening algal growth inhibition test with Dunaliella sp. Jpn. J. Environ. Toxicol., 15, 11-16 (in Japanese with English summary).
Strain(s): 3744 
DOI: 10.11403/jset.15.11

Watanuki, T. 1998 Organisms as measured by nuclear magnetic resonance spectrometer. Bull. Kanagawa P. H. Lab., 28, 1-15 (in Japanese).
Keywords: 1H-NMR; 13C-NMR; 31P-NMR; 137Cs-NMR
Strain(s): 3744 

Utsunomiya, A., Watanuki, T., Matsushita, K., Tomita, I 1997 Toxic effects of linear alkylbenzene sulfonate, quaternaryalkylammonium chloride and their complexes on Dunaliella sp. and Chlorella pyrenoidosa. Environ. Toxicol. Chem., 16, 1247-1254.
Keywords: Linear alkylbenzene sulfonate; Cationic surfactant; Complex Dunaliella Chlorella
Strain(s): 3744 
DOI: 10.1002/etc.5620160621

Utsunomiya, A., Watanuki, T., Matsushita, K., Nishina, M., Tomita, I 1997 Assessment of the toxicity of linear alkylbenzene sulfonate and quaternary alkylammonium chloride by measuring 13C-glycerol in Dunaliella sp. Chemosphere, 35, 2479-2490.
Keywords: Surfactant; Toxicity; LAS; Quaternary ammonium chloride; Glycerol; 13C-glycerol; Dunaliella sp; 13C-NMR; EC50-24h
Strain(s): 3744 
DOI: 10.1016/S0045-6535(97)00316-0

Watanuki, T., Iijima, I., Matsushita, K., Nishina, M., Sumiya, K., Kato, K. 1996 Incorporation of 13C labelled carbon into glycerol in the halotolerant alga Dunaliella. JSMRM, 7, 109-112 (in Japanese).
Keywords: halotolerant species; 13C-enriched sodium bicarbonate; glycerol
Strain(s): 3744 

Watanuki, T., Matsushita, K., Nishina, M., Hori, E., Kato, K. 1994 J. Phys. Fit. Nutr. Immunol., 40-44 (in Japanese).
Strain(s): 3744 

Watanuki, T. 1994 Response to salt stress of Antarctic Dunaliella, as measured by nuclear magnetic resonance spectroscopy. JSMRM, 5, 118-120 (in Japanese).
Keywords: antarctic; Dunaliella; high salt lake; salt stress; NMR; glycerol
Strain(s): 3744 

Watanuki, T., Matsushita, K., Kato, K. 1993 Response of Antarctic alga to salt stress, as measured by nuclear magnetic resonance spectroscopy. Seibutsu-Butsuri, 33, 38-41 (in Japanese).
Strain(s): 3744 
DOI: 10.2142/biophys.33.228

Watanuki, T., Ohno, M., Nakamura, S. 1987 Growth characteristics of Dunaliella sp., unicellular green alga isolated from a salt lake along the coastal region of Lüetzow-Holm bay, Antarctica. Rep. Usa mar. biol. Inst., Kouchi Univ., 9, 139-147.
Keywords: Dunaliella sp.; Green algae; Saline lake; Antarctica
Strain(s): 3744 

Watanuki, T. 1979 Isolation and culture of Antarctic diatoms from the saline lakes in the Soya coast, east Antarctica. Memoirs of National Institute of Polar Research, Special issue 11, 35-41.
Strain(s): 3744 

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