Strain Data

Strain number		NIES-3715
Phylum		Heterokontophyta
Class		Bacillariophyceae
Scientific name		Chaetoceros tenuissimus Meunier
Synonym
Former name
Common name		Centric diatom
Locality (Date of collection)		off Maikohama, Harima-nada, Seto Inland Sea, Hyogo Japan (2002-08-10)
Latitude / Longitude		34.62888888 / 135.03525
Habitat (Isolation source)		Marine (Seawater)
History		< Kimura, Kei < Tomaru, Yuji
Isolator (Date of isolation)		Tomaru, Yuji (2002-08-11)
Identified by		Suzuki, Hidekazu; Nagumo, Tamotsu
State of strain		Cryopreservation; Unialgal; Clonal; Axenic
Culture condition (Preculture condition)		Medium: mSWM-3 Temperature: 15 C Light intensity: 9-11 µmol photons/m²/sec, L/D cycle: 10L:14D Duration: 21 D
Gene information		Nuclear genome ( BLLK01000001-BLLK01000085 ) , Mitochondrial genome ( LC537470 ) , Plastid genome ( LC537471 ) , 18S rRNA ( AB847417 )
Cell size (min - max)
Organization		Unicellular
Characteristics		Red tide ; The virus which infects with this diatom strain has been reported (Shirai et al. 2008).
Other strain no.		Other collection strain no. : CCAP 1010/43 Other strain no. : 2-10
Remarks		Cryopreserved; Axenic
Movie

Reference
Tomaru, Y., Yamaguchi, H., Miki, T. 2021 Growth rate-dependent cell death of diatoms due to viral infection and their subsequent coexistence in a semi-continuous culture system. Microbes Environ., 36, ME20116 (article ID). Keywords: diatom; population dynamics; viral infection Strain(s): 3715 PubMed: 33390375 DOI: 10.1264/jsme2.ME20116 Tomaru, Y., Kimura, K. 2020 Novel protocol for estimating viruses specifically infecting the marine planktonic diatoms. Diversity-Basel, 12, 225 (article ID). Keywords: marine diatom; Chaetoceros tenuissimus; Chaetoceros setoensis; host-virus specificity; RdRp; ssRNA virus; ssDNA virus; viral genome Strain(s): 3712, 3714, 3715 DOI: 10.3390/d12060225 Hano, T., Tomaru, Y. 2019 Metabolomics-based approach to explore growth phase-dependent markers in cultured diatom Chaetoceros tenuissimus. J. Chromatogr. B, 1128, 121779 (article ID). Keywords: Diatom; Metabolomics; Gas chromatography mass spectrometry; Liquid chromatography tandem mass spectrometry; Growth phase Strain(s): 3715 PubMed: 31499295 DOI: 10.1016/j.jchromb.2019.121779 Tomaru, Y., Toyoda, K., Kimura, K. 2018 Occurrence of the planktonic bloom-forming marine diatom Chaetoceros tenuissimus Meunier and its infectious viruses in western Japan. Hydrobiologia, 805, 221-230. Keywords: Algal viruses; Primary producers; Marine algae; Real-time quantitative PCR; Restingstage cells Strain(s): 3714, 3715 DOI: 10.1007/s10750-017-3306-0 Yamada, Y., Tomaru, Y., Fukuda, H., Nagata, T. 2018 Aggregate formation during the viral lysis of a marine diatom. Front. Mar. Sci., 15, 167 (article ID). Keywords: aggregate; diatom–virus interaction; particle size distribution; settling velocity; material cycle Strain(s): 3715 DOI: 10.3389/fmars.2018.00167 Tomaru, Y., Shirai, Y., Toyoda, K., Nagasaki, K. 2011 Isolation and characterisation of a single-stranded DNA virus infecting the marine planktonic diatom Chaetoceros tenuissimus. Aquat. Microb. Ecol., 64, 175-184. Keywords: Bacilladnavirus; Chaetoceros; Diatom; ssDNA; Virus; Rod-shaped particle Strain(s): 3714, 3715 DOI: 10.3354/ame01517 Toyoda, K., Nagasaki, K., Tomaru, Y. 2010 Application of real-time PCR assay for detection and quantification of bloom-forming diatom Chaetoceros tenuissimus Meunier. Plankton Benthos Res., 5, 56-61. Keywords: Chaetoceros tenuissimus; detection; diatom; enumeration; TaqMan-based real-time PCR Strain(s): 3715 DOI: 10.3800/pbr.5.56 Shirai, Y., Tomaru, Y., Takao, Y., Suzuki, H., Nagumo, T., Nagasaki, K. 2008 Isolation and characterization of a single-stranded DNA virus infecting the marine planktonic diatom Chaetoceros tenuissimus Meunier. Appl. Environ. Microbiol., 74, 4022-4027. Strain(s): 3715 PubMed: 18469125 DOI: 10.1128/AEM.00509-08

Reference

Tomaru, Y., Yamaguchi, H., Miki, T. 2021 Growth rate-dependent cell death of diatoms due to viral infection and their subsequent coexistence in a semi-continuous culture system. Microbes Environ., 36, ME20116 (article ID).
Keywords: diatom; population dynamics; viral infection
Strain(s): 3715
PubMed: 33390375
DOI: 10.1264/jsme2.ME20116

Tomaru, Y., Kimura, K. 2020 Novel protocol for estimating viruses specifically infecting the marine planktonic diatoms. Diversity-Basel, 12, 225 (article ID).
Keywords: marine diatom; Chaetoceros tenuissimus; Chaetoceros setoensis; host-virus specificity; RdRp; ssRNA virus; ssDNA virus; viral genome
Strain(s): 3712, 3714, 3715
DOI: 10.3390/d12060225

Hano, T., Tomaru, Y. 2019 Metabolomics-based approach to explore growth phase-dependent markers in cultured diatom Chaetoceros tenuissimus. J. Chromatogr. B, 1128, 121779 (article ID).
Keywords: Diatom; Metabolomics; Gas chromatography mass spectrometry; Liquid chromatography tandem mass spectrometry; Growth phase
Strain(s): 3715
PubMed: 31499295
DOI: 10.1016/j.jchromb.2019.121779

Tomaru, Y., Toyoda, K., Kimura, K. 2018 Occurrence of the planktonic bloom-forming marine diatom Chaetoceros tenuissimus Meunier and its infectious viruses in western Japan. Hydrobiologia, 805, 221-230.
Keywords: Algal viruses; Primary producers; Marine algae; Real-time quantitative PCR; Restingstage cells
Strain(s): 3714, 3715
DOI: 10.1007/s10750-017-3306-0

Yamada, Y., Tomaru, Y., Fukuda, H., Nagata, T. 2018 Aggregate formation during the viral lysis of a marine diatom. Front. Mar. Sci., 15, 167 (article ID).
Keywords: aggregate; diatom–virus interaction; particle size distribution; settling velocity; material cycle
Strain(s): 3715
DOI: 10.3389/fmars.2018.00167

Tomaru, Y., Shirai, Y., Toyoda, K., Nagasaki, K. 2011 Isolation and characterisation of a single-stranded DNA virus infecting the marine planktonic diatom Chaetoceros tenuissimus. Aquat. Microb. Ecol., 64, 175-184.
Keywords: Bacilladnavirus; Chaetoceros; Diatom; ssDNA; Virus; Rod-shaped particle
Strain(s): 3714, 3715
DOI: 10.3354/ame01517

Toyoda, K., Nagasaki, K., Tomaru, Y. 2010 Application of real-time PCR assay for detection and quantification of bloom-forming diatom Chaetoceros tenuissimus Meunier. Plankton Benthos Res., 5, 56-61.
Keywords: Chaetoceros tenuissimus; detection; diatom; enumeration; TaqMan-based real-time PCR
Strain(s): 3715
DOI: 10.3800/pbr.5.56

Shirai, Y., Tomaru, Y., Takao, Y., Suzuki, H., Nagumo, T., Nagasaki, K. 2008 Isolation and characterization of a single-stranded DNA virus infecting the marine planktonic diatom Chaetoceros tenuissimus Meunier. Appl. Environ. Microbiol., 74, 4022-4027.
Strain(s): 3715
PubMed: 18469125
DOI: 10.1128/AEM.00509-08

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