Strain number NIES-546  
Phylum Chlorophyta  
Class Chlorophyceae  
Scientific name Volvulina steinii Playfair  
Synonym  
Former name  
Common name Green alga  
Locality (Date of collection) Hayama, Kanagawa, Japan (1980-12-**)  
Latitude / Longitude 35.271286 / 139.600715 
Habitat (Isolation source) Freshwater (Paddy soil)  
History < Nozaki, Hisayoshi  
Isolator (Date of isolation) Nozaki, Hisayoshi (1981-01-07)  
Identified by Nozaki, Hisayoshi  
State of strain Cryopreservation; Unialgal; Clonal; Axenic[2017 Dec]  
Culture condition
(Preculture condition)
Medium:  VTAC  
Temperature:  20 C
Light intensity:  21 µmol photons/m2/sec, L/D cycle:  10L:14D
Duration:  2 M  
Gene information act1 ( AB973309 ) , act2 ( AB973312 )  
Cell size (min - max)  
Organization Colonial; Flagellate 
Characteristics Phototaxis ; Heterothallic ; Mating type (+) ; Crosses with NIES-545  
Other strain no. Other strain no. : 1107-8 (+)  
Remarks Cryopreserved; Axenic 
Movie  
Reference
Phylogenetic analysis of NAP, an unconventional actin of the Volvocales. 2015 Phylogenetic analysis of NAP, an unconventional actin of the Volvocales. Plant Syst. Evol., 301, 1725-1733.
Keywords: Volvocales; Divergent actin
Strain(s): 3515465767173374 
DOI: 10.1007/s00606-014-1187-5

Kato-Minoura, T., Okumura, M., Hirono, M., Kamiya, R. 2003 A novel family of unconventional actins in Volvocalean algae. J. Mol. Evol., 57, 555-561.
Keywords: Chlamydomonas; phylogeny; Volvocales
Strain(s): 158351546569 
PubMed: 14738314
DOI: 10.1007/s00239-003-2509-3

Mori, F., Erata, M., Watanabe, M. M. 2002 Cryopreservation of cyanobacteria and green algae in the NIES-Collection. Microbiol. Cult. Coll., 18, 45-55.
Keywords: cryopreservation; cyanobacteria; green algae; FDA staining method; NIES-Collection
Strain(s): 192122232425262728293031323334353637383940414445465051525354555658596061626364656667686970737475767778798081878990919293949596979899100101102103104105106107108109110111112119120122123125127128129130131132133134135137138139144147151152153154155156157158159160162163164165166167168170171172173174175176177178179180181182183185186187188189191192193194197198199200201202203204205206207208209210211212213214215216217224226227228229230231232241242243244245246248257259261263266267268287288289290294295297298299300301302303305306308309310312313329334337338339340341342349351359360361362375378379380382384385390394396397398415416418419421422423424425426427428429430431432433434436437438439440446447448449450451452453454455456457458459460464468469474478479480481503504505506507509510512514515522523524527528529530531532536537538539540541542543544545546564565566567568569570571572573574575576577578579580581582583584585592593594595596597598604610628630631632633634635636637638639640641642643644645646647648649650651652653654655656657658659660661662663664665666667672685686687688689690691692693694717718719720721722723724725726727728729 

Nozaki, H. & Krienitz, L. 2001 Morphology and phylogeny of Eudorina minodii (Chodat) Nozaki et Krienitz, comb. nov. (Volvocales, Chlorophyta) from Germany. Eur. J. Phycol., 36, 23-28.
Keywords: Eudorina minodii comb. nov.; molecular phylogeny; morphology; Pandorina minodii; taxonomy; Volvocales
Strain(s): 351546576717856 
DOI: 10.1080/09670260110001735158

Nozaki, H. 1982 Morphology and reproduction of Japanese Volvulina steinii (Chlorophyta, Volvocales). J. Jpn. Bot., 57, 105-113, pls. 3-4.
Strain(s): 545546896897898 

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