Strain number NIES-3637  
Phylum Dinophyta  
Class Dinophyceae  
Scientific name Coolia malayensis Leaw, P.-T.Lim & Usup  
Former name  
Common name Dinoflagellate  
Locality (Date of collection) Teniya, Nago, Okinawa, Japan (2013-08-**)  
Latitude / Longitude 26.56312500 / 128.14150555 
Habitat (Isolation source) Marine (Seaweed)  
History < Wakeman, Kevin  
Isolator (Date of isolation) Wakeman, Kevin (2013-08-**)  
Identified by Wakeman, Kevin  
State of strain Subculture; Unialgal; Non-axenic  
Culture condition
(Preculture condition)
Medium:  f/2  
Temperature:  20 C
Light intensity:  10-17 µmol photons/m2/sec, L/D cycle:  10L:14D
Duration:  1 M  
Gene information 18S-ITS1-5.8S-ITS2 ( KJ933433 ) , 18S-ITS1-5.8S-ITS2-28S ( KJ933432 KJ933434 KJ933435 ) , 28S rRNA ( KJ933436 KJ933437 KJ933438 KJ933439 )  
Cell size (min - max)  
Organization Unicellular 
Characteristics Toxicity (Wakeman et al. 2011) ; Benthic ; Epiphytic  
Other strain no. Other strain no. : C1  
Remarks Toxicity; Fragile species to transportation stresses; Read and agree "How to order 4.1" 
Phua, Y. H., Roy, M. C., Lemer ,S., Husnik, F., Wakeman, K. C. 2021 Diversity and toxicity of Pacific strains of the benthic dinoflagellate Coolia (Dinophyceae), with a look at the Coolia canariensis species complex. Harmful Algae, 109, 102120 (article ID).
Keywords: Cooliatoxin; Cryptic species; Harmful algal bloom; High-resolution MS; Phylogeny; Taxonomy
Strain(s): 3637 
PubMed: 34815025
DOI: 10.1016/j.hal.2021.102120

Wakeman, K. C., Yamaguchi, A., Roy, M. C., Jenke-Kodama, H. 2015 Morphology, phylogeny and novel chemical compounds from Coolia malayensis (Dinophyceae) from Okinawa, Japan. Harmful Algae, 44, 8-19.
Keywords: Coolia malayensis; Cooliatoxin; High-resolution MS; Morphology; NanoLC/MS; Molecular phylogeny
Strain(s): 3637 
DOI: 10.1016/j.hal.2015.02.009

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