Strain Data

Strain number		NIES-254
Phylum		Chlorophyta
Class		Pyramimonadophyceae
Scientific name		Pyramimonas parkeae R.E.Norris & B.R.Pearson
Synonym
Former name
Common name
Locality (Date of collection)		Hachijo Isl., Tokyo, Japan (1984-04-17)
Latitude / Longitude		33.110916 / 139.798936
Habitat (Isolation source)		Marine (Seawater)
History		< Suda, Shoichiro
Isolator (Date of isolation)		Suda, Shoichiro (1984-04-21)
Identified by		Suda, Shoichiro
State of strain		Subculture; Unialgal; Clonal; Axenic[2018 Feb]
Culture condition (Preculture condition)		Medium: ESM Temperature: 20 C Light intensity: 13-16 µmol photons/m²/sec, L/D cycle: 10L:14D Duration: 1 M
Gene information		beta-tubulin ( AB491640 ) , psbO ( AB293976 )
Cell size (min - max)
Organization		Unicellular; Flagellate
Characteristics		Red tide ; Prey for Oxyrrhis marina NIES-494 and NIES-3607
Other strain no.		Other strain no. : 8-25-2
Remarks		Axenic
Movie

Reference
Hamana, K., Furuchi, T., Hayashi, H., Uemura, T., Niitsu, M. 2023 Additional polyamine analysis of the algae belonging to the phyla Glaucophyta, Rhodophyta and Chlorophyta. ─Polyamine analysis of algae V─ Microb. Resour. Syst., 39, 33-40. Keywords: alga; Chlorophyta; Glaucophyta; polyamine; Rhodophyta Strain(s): 226, 250, 254, 550, 551, 626, 1298, 1332, 1703, 1836, 2137, 2163, 2164, 2165, 2166, 2252, 2353, 2894, 3377, 3645, 3867, 3891, 3892, 4107, 4390, 4452 Bock, N. A., Charvet, S., Burns, J., Gyaltshen, Y., Rozenberg, A., Duhamel ,S., Kim, E. 2021 Experimental identification and in silico prediction of bacterivory in green algae. ISME J., 15, 1987-2000. Strain(s): 254, 296, 626, 995 PubMed: 33649548 DOI: 10.1038/s41396-021-00899-w Kashiyama, Y. et al. 2019 Taming chlorophylls by early eukaryotes underpinned algal interactions and the diversification of the eukaryotes on the oxygenated Earth. ISME J., 13, 1899-1910. Strain(s): 8, 254, 274, 286, 324, 331, 353, 381, 494, 623, 624, 997, 1012, 1013, 1015, 1320, 1330, 1333, 1334, 1335, 1372, 1377, 1388, 1390, 1396, 1397, 1408, 1438, 1439, 1441, 1964, 2008, 2142, 2144, 2149, 2300, 2305, 2325, 2433, 2498, 2502, 2566, 2584, 2586, 2589, 2590, 2677, 2878, 3356, 3374, 3775 PubMed: 30809012 DOI: 10.1038/s41396-019-0377-0 Satjarak, A. & Graham, L. E. 2017 Comparative DNA sequence analyses of Pyramimonas parkeae (Prasinophyceae) chloroplast genomes. J. Phycol., 53, 415-424. Keywords: chloroplast DNA variation; chloroplast genome; intraspecific variation; prasinophyte; Pyramimonas parkeae Strain(s): 254 PubMed: 28130930 DOI: 10.1111/jpy.12515 Kunugi, M., Satoh. S., Ihara. K., Shibata. K., Yamagishi. Y., Kogame. K., Obokata. J., Takabayashi. A., Tanaka. A. 2016 Evolution of green plants accompanied changes in light-harvesting systems. Plant Cell Physiol., 57, 1231-1243. Keywords: Evolution; Green algae; Land plants; Light adaptation; Photosystem Strain(s): 254, 1419, 2541, 2582 PubMed: 27057002 DOI: 10.1093/pcp/pcw071 Yamagishi, T., Kurihara, A., Kawai, H. 2015 A ribbon-like structure in the ejective organelle of the green microalga Pyramimonas parkeae (Prasinophyceae) consists of core histones and polymers containing N-acetyl-glucosamine. Protist, 166, 522-533. Keywords: Core histone; ejectisome; N-acetyl-glucosamine; Pyramimonas parkeae Strain(s): 251, 254 PubMed: 26398336 DOI: 10.1016/j.protis.2015.08.003 Alamsjah, M. A., Hirao, S., Ishibashi, F., Oda, T., Fujita, Y. 2008 Algicidal activity of polyunsaturated fatty acids derived from Ulva fasciata and U. pertusa (Ulvaceae, Chlorophyta) on phytoplankton. J. Appl. Phycol., 20, 713-720. Keywords: Algicidal compound; Red tide phytoplankton; Ulva fasciata; Ulva pertusa Strain(s): 1, 3, 7, 221, 251, 252, 254, 359, 381, 462, 486 DOI: 10.1007/s10811-007-9257-5 Takahashi, F., Okabe, Y., Nakada, T., Sekimoto, H., Ito, M., Kataoka, H. & Nozaki, H. 2007 Origins of the secondary plastids of Euglenophyta and Chlorarachniophyta as revealed by an analysis of the plastid-targeting, nuclear-encoded gene psbO. J. Phycol., 43, 1302-1309. Keywords: alanine-X-alanine motif; Chlorarachniophyta; Euglenophyta; phylogeny; plastids; psbO; secondary endosymbiosis; transit peptide; thylakoid-targeting domain Strain(s): 67, 203, 254, 381, 483, 533, 624, 656 DOI: 10.1111/j.1529-8817.2007.00411.x Hamana, K., Aizaki T., Arai, E., Saito, A., Uchikata, K., Ohnishi, H. 2004 Distribution of norspermidine s a cellular polyamine within micro green algae including non-photosynthetic achlorophyllous Polytoma, Polytomella, Prototheca and Helicosporidium. J. Gen. Appl. Microbiol., 50, 289-295. Keywords: Chlorophyta; green alga; Helicosporidium; norspermidine; polyamine; Polytoma; Polytomella; Prototheca Strain(s): 18, 251, 254, 255, 296, 329, 483, 486, 536 Kim, Y.-S., Oyaizu, H., Matsumoto, S, Watanabe, M. M., Nozaki, H. 1994 Chloroplast small-subunit ribosomal RNA gene sequence from Chlamydomonas parkeae (Chlorophyta) : molecular phylogeny of a green alga with a peculiar pigment composition. Eur. J. Phycol., 29, 213-217. Keywords: Chlamydomonas parkeae; Chlorophyta; chloroplast small-subunit (SSU) rRNA; phylogeny; pigment composition Strain(s): 228, 254, 440, 468, 666 DOI: 10.1080/09670269400650661 Sasa, T., Takaichi, S. Hatakeyama, N., Watanabe, M. M. 1992 A novel carotenoid ester, loroxanthin dodecenoate, from Pyramimonas parkeae (Prasinophyceae) and a chlorarachniophycean alga. Plant Cell Physiol., 33, 921-925. Keywords: (3R,3'R,6'R)-β,ε-carotene-3,19,3'-triol 19-(2-dodecanoate); chlorarachniophyte; 1H-NMR; loroxanthin dodecenoate; Pyramimonas parkeae Strain(s): 254 DOI: 10.1093/oxfordjournals.pcp.a078342 Kohata, K., Watanabe, M., Yamanaka, K. 1991 Highly sensitive determination of photosynthetic pigments in marine in situ samples by high-performance liquid chromatography. J. Chromatogr. A 558, 131-140. Strain(s): 6, 7, 254, 323, 353 DOI: 10.1016/0021-9673(91)80118-Z Kohata, K. & Watanabe, M. 1989 Diel changes in the composition of photosynthetic pigments and cellular carbon and nitrogen in Pyramimonas parkeae (Prasinophyceae). J. Phycol., 25, 377-385. Keywords: carotenoids; cellurar carbon; chlorophyll; diel change; laboratory culture tank; light-dark cycles; loroxanthin dodecenoate; Pyremimonas parkeae Strain(s): 254 DOI: 10.1111/j.1529-8817.1989.tb00134.x

Reference

Hamana, K., Furuchi, T., Hayashi, H., Uemura, T., Niitsu, M. 2023 Additional polyamine analysis of the algae belonging to the phyla Glaucophyta, Rhodophyta and Chlorophyta. ─Polyamine analysis of algae V─ Microb. Resour. Syst., 39, 33-40.
Keywords: alga; Chlorophyta; Glaucophyta; polyamine; Rhodophyta
Strain(s): 226, 250, 254, 550, 551, 626, 1298, 1332, 1703, 1836, 2137, 2163, 2164, 2165, 2166, 2252, 2353, 2894, 3377, 3645, 3867, 3891, 3892, 4107, 4390, 4452

Bock, N. A., Charvet, S., Burns, J., Gyaltshen, Y., Rozenberg, A., Duhamel ,S., Kim, E. 2021 Experimental identification and in silico prediction of bacterivory in green algae. ISME J., 15, 1987-2000.
Strain(s): 254, 296, 626, 995
PubMed: 33649548
DOI: 10.1038/s41396-021-00899-w

Kashiyama, Y. et al. 2019 Taming chlorophylls by early eukaryotes underpinned algal interactions and the diversification of the eukaryotes on the oxygenated Earth. ISME J., 13, 1899-1910.
Strain(s): 8, 254, 274, 286, 324, 331, 353, 381, 494, 623, 624, 997, 1012, 1013, 1015, 1320, 1330, 1333, 1334, 1335, 1372, 1377, 1388, 1390, 1396, 1397, 1408, 1438, 1439, 1441, 1964, 2008, 2142, 2144, 2149, 2300, 2305, 2325, 2433, 2498, 2502, 2566, 2584, 2586, 2589, 2590, 2677, 2878, 3356, 3374, 3775
PubMed: 30809012
DOI: 10.1038/s41396-019-0377-0

Satjarak, A. & Graham, L. E. 2017 Comparative DNA sequence analyses of Pyramimonas parkeae (Prasinophyceae) chloroplast genomes. J. Phycol., 53, 415-424.
Keywords: chloroplast DNA variation; chloroplast genome; intraspecific variation; prasinophyte; Pyramimonas parkeae
Strain(s): 254
PubMed: 28130930
DOI: 10.1111/jpy.12515

Kunugi, M., Satoh. S., Ihara. K., Shibata. K., Yamagishi. Y., Kogame. K., Obokata. J., Takabayashi. A., Tanaka. A. 2016 Evolution of green plants accompanied changes in light-harvesting systems. Plant Cell Physiol., 57, 1231-1243.
Keywords: Evolution; Green algae; Land plants; Light adaptation; Photosystem
Strain(s): 254, 1419, 2541, 2582
PubMed: 27057002
DOI: 10.1093/pcp/pcw071

Yamagishi, T., Kurihara, A., Kawai, H. 2015 A ribbon-like structure in the ejective organelle of the green microalga Pyramimonas parkeae (Prasinophyceae) consists of core histones and polymers containing N-acetyl-glucosamine. Protist, 166, 522-533.
Keywords: Core histone; ejectisome; N-acetyl-glucosamine; Pyramimonas parkeae
Strain(s): 251, 254
PubMed: 26398336
DOI: 10.1016/j.protis.2015.08.003

Alamsjah, M. A., Hirao, S., Ishibashi, F., Oda, T., Fujita, Y. 2008 Algicidal activity of polyunsaturated fatty acids derived from Ulva fasciata and U. pertusa (Ulvaceae, Chlorophyta) on phytoplankton. J. Appl. Phycol., 20, 713-720.
Keywords: Algicidal compound; Red tide phytoplankton; Ulva fasciata; Ulva pertusa
Strain(s): 1, 3, 7, 221, 251, 252, 254, 359, 381, 462, 486
DOI: 10.1007/s10811-007-9257-5

Takahashi, F., Okabe, Y., Nakada, T., Sekimoto, H., Ito, M., Kataoka, H. & Nozaki, H. 2007 Origins of the secondary plastids of Euglenophyta and Chlorarachniophyta as revealed by an analysis of the plastid-targeting, nuclear-encoded gene psbO. J. Phycol., 43, 1302-1309.
Keywords: alanine-X-alanine motif; Chlorarachniophyta; Euglenophyta; phylogeny; plastids; psbO; secondary endosymbiosis; transit peptide; thylakoid-targeting domain
Strain(s): 67, 203, 254, 381, 483, 533, 624, 656
DOI: 10.1111/j.1529-8817.2007.00411.x

Hamana, K., Aizaki T., Arai, E., Saito, A., Uchikata, K., Ohnishi, H. 2004 Distribution of norspermidine s a cellular polyamine within micro green algae including non-photosynthetic achlorophyllous Polytoma, Polytomella, Prototheca and Helicosporidium. J. Gen. Appl. Microbiol., 50, 289-295.
Keywords: Chlorophyta; green alga; Helicosporidium; norspermidine; polyamine; Polytoma; Polytomella; Prototheca
Strain(s): 18, 251, 254, 255, 296, 329, 483, 486, 536

Kim, Y.-S., Oyaizu, H., Matsumoto, S, Watanabe, M. M., Nozaki, H. 1994 Chloroplast small-subunit ribosomal RNA gene sequence from Chlamydomonas parkeae (Chlorophyta) : molecular phylogeny of a green alga with a peculiar pigment composition. Eur. J. Phycol., 29, 213-217.
Keywords: Chlamydomonas parkeae; Chlorophyta; chloroplast small-subunit (SSU) rRNA; phylogeny; pigment composition
Strain(s): 228, 254, 440, 468, 666
DOI: 10.1080/09670269400650661

Sasa, T., Takaichi, S. Hatakeyama, N., Watanabe, M. M. 1992 A novel carotenoid ester, loroxanthin dodecenoate, from Pyramimonas parkeae (Prasinophyceae) and a chlorarachniophycean alga. Plant Cell Physiol., 33, 921-925.
Keywords: (3R,3'R,6'R)-β,ε-carotene-3,19,3'-triol 19-(2-dodecanoate); chlorarachniophyte; 1H-NMR; loroxanthin dodecenoate; Pyramimonas parkeae
Strain(s): 254
DOI: 10.1093/oxfordjournals.pcp.a078342

Kohata, K., Watanabe, M., Yamanaka, K. 1991 Highly sensitive determination of photosynthetic pigments in marine in situ samples by high-performance liquid chromatography. J. Chromatogr. A 558, 131-140.
Strain(s): 6, 7, 254, 323, 353
DOI: 10.1016/0021-9673(91)80118-Z

Kohata, K. & Watanabe, M. 1989 Diel changes in the composition of photosynthetic pigments and cellular carbon and nitrogen in Pyramimonas parkeae (Prasinophyceae). J. Phycol., 25, 377-385.
Keywords: carotenoids; cellurar carbon; chlorophyll; diel change; laboratory culture tank; light-dark cycles; loroxanthin dodecenoate; Pyremimonas parkeae
Strain(s): 254
DOI: 10.1111/j.1529-8817.1989.tb00134.x

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