Strain number NIES-254  
Phylum Chlorophyta  
Class Prasinophyceae  
Scientific name Pyramimonas parkeae R.E.Norris & B.R.Pearson  
Former name  
Common name  
Locality (Date of collection) Hachijo Isl., Tokyo, Japan (1984-04-17)  
Latitude / Longitude 33.110916 / 139.798936 
Habitat (Isolation source) Marine (Seawater)  
History < Suda, Shoichiro  
Isolator (Date of isolation) Suda, Shoichiro (1984-04-21)  
Identified by Suda, Shoichiro  
State of strain Subculture; Unialgal; Clonal; Axenic[2018 Feb]  
Culture condition
(Preculture condition)
Medium:  ESM  
Temperature:  20 C
Light intensity:  13-16 µmol photons/m2/sec, L/D cycle:  10L:14D
Duration:  1 M  
Gene information beta-tubulin ( AB491640 ) , psbO ( AB293976 )  
Cell size (min - max)  
Organization Unicellular; Flagellate 
Characteristics Red tide ; Prey for Oxyrrhis marina (NIES-494, 3607)  
Other strain no. Other strain no. : 8-25-2  
Remarks Axenic 
Bock, N. A., Charvet, S., Burns, J., Gyaltshen, Y., Rozenberg, A., Duhamel ,S., Kim, E. 2021 Experimental identification and in silico prediction of bacterivory in green algae. ISME J., 15, 1987-2000.
Strain(s): 254296626995 
PubMed: 33649548
DOI: 10.1038/s41396-021-00899-w

Kashiyama, Y. et al. 2019 Taming chlorophylls by early eukaryotes underpinned algal interactions and the diversification of the eukaryotes on the oxygenated Earth. ISME J., 13, 1899-1910.
Strain(s): 8254274286324331353381494623624997101210131015132013301333133413351372137713881390139613971408143814391441186819642008214221442149230023052325241124332498250225662584258625892590267728783374360437753876 
PubMed: 30809012
DOI: 10.1038/s41396-019-0377-0

Satjarak, A., Graham, L. E. 2017 Comparative DNA sequence analyses of Pyramimonas parkeae (Prasinophyceae) chloroplast genomes. J. Phycol., 53, 415-424.
Keywords: chloroplast DNA variation; chloroplast genome; intraspecific variation; prasinophyte; Pyramimonas parkeae
Strain(s): 254 
PubMed: 28130930
DOI: 10.1111/jpy.12515

Kunugi, M., Satoh. S., Ihara. K., Shibata. K., Yamagishi. Y., Kogame. K., Obokata. J., Takabayashi. A., Tanaka. A. 2016 Evolution of green plants accompanied changes in light-harvesting systems. Plant Cell Physiol., 57, 1231-1243.
Keywords: Evolution; Green algae; Land plants; Light adaptation; Photosystem
Strain(s): 254141925412582 
PubMed: 27057002
DOI: 10.1093/pcp/pcw071

Yamagishi, T., Kurihara, A., Kawai, H. 2015 A ribbon-like structure in the ejective organelle of the green microalga Pyramimonas parkeae (Prasinophyceae) consists of core histones and polymers containing N-acetyl-glucosamine. Protist, 166, 522-533.
Keywords: Core histone; ejectisome; N-acetyl-glucosamine; Pyramimonas parkeae
Strain(s): 251254 
PubMed: 26398336
DOI: 10.1016/j.protis.2015.08.003

Alamsjah, M. A., Hirao, S., Ishibashi, F., Oda, T., Fujita, Y. 2008 Algicidal activity of polyunsaturated fatty acids derived from Ulva fasciata and U. pertusa (Ulvaceae, Chlorophyta) on phytoplankton. J. Appl. Phycol., 20, 713-720.
Keywords: Algicidal compound; Red tide phytoplankton; Ulva fasciata; Ulva pertusa
Strain(s): 137221251252254359381462486 
DOI: 10.1007/s10811-007-9257-5

Takahashi, F., Okabe, Y., Nakada, T., Sekimoto, H., Ito, M., Kataoka, H. & Nozaki, H. 2007 Origins of the secondary plastids of Euglenophyta and Chlorarachniophyta as revealed by an analysis of the plastid-targeting, nuclear-encoded gene psbO. J. Phycol., 43, 1302-1309.
Keywords: alanine-X-alanine motif; Chlorarachniophyta; Euglenophyta; phylogeny; plastids; psbO; secondary endosymbiosis; transit peptide; thylakoid-targeting domain
Strain(s): 67203254381483533624656 
DOI: 10.1111/j.1529-8817.2007.00411.x

Hamana, K., Aizaki T., Arai, E., Saito, A., Uchikata, K., Ohnishi, H. 2004 Distribution of norspermidine s a cellular polyamine within micro green algae including non-photosynthetic achlorophyllous Polytoma, Polytomella, Prototheca and Helicosporidium. J. Gen. Appl. Microbiol., 50, 289-295.
Keywords: Chlorophyta; green alga; Helicosporidium; norspermidine; polyamine; Polytoma; Polytomella; Prototheca
Strain(s): 18251254255296329483486536 

Kim, Y.-S., Oyaizu, H., Matsumoto, S, Watanabe, M. M., Nozaki, H. 1994 Chloroplast small-subunit ribosomal RNA gene sequence from Chlamydomonas parkeae (Chlorophyta) : molecular phylogeny of a green alga with a peculiar pigment composition. Eur. J. Phycol., 29, 213-217.
Keywords: Chlamydomonas parkeae; Chlorophyta; chloroplast small-subunit (SSU) rRNA; phylogeny; pigment composition
Strain(s): 228254440468666 
DOI: 10.1080/09670269400650661

Sasa, T., Takaichi, S. Hatakeyama, N., Watanabe, M. M. 1992 A novel carotenoid ester, loroxanthin dodecenoate, from Pyramimonas parkeae (Prasinophyceae) and a chlorarachniophycean alga. Plant Cell Physiol., 33, 921-925.
Keywords: (3R,3'R,6'R)-β,ε-carotene-3,19,3'-triol 19-(2-dodecanoate); chlorarachniophyte; 1H-NMR; loroxanthin dodecenoate; Pyramimonas parkeae
Strain(s): 254 
DOI: 10.1093/oxfordjournals.pcp.a078342

Kohata, K., Watanabe, M., Yamanaka, K. 1991 Highly sensitive determination of photosynthetic pigments in marine in situ samples by high-performance liquid chromatography. J. Chromatogr. A 558, 131-140.
Strain(s): 67254323353 
DOI: 10.1016/0021-9673(91)80118-Z

Kohata, K., Watanabe, M. 1989 Diel changes in the composition of photosynthetic pigments and cellular carbon and nitrogen in Pyramimonas parkeae (Prasinophyceae). J. Phycol., 25, 377-385.
Keywords: carotenoids; cellurar carbon; chlorophyll; diel change; laboratory culture tank; light-dark cycles; loroxanthin dodecenoate; Pyremimonas parkeae
Strain(s): 254 
DOI: 10.1111/j.1529-8817.1989.tb00134.x

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