Strain number NIES-18  
Phylum Chlorophyta  
Class Prasinophyceae  
Scientific name Tetraselmis cordiformis (Carter) Stein  
Synonym Cryptoglena cordiformis H.J.Carter; Carteria cordiformis (H.J.Carter) Diesing 1866; Pteromonas angulosa var. cordiformis (H.J.Carter) Philipose 1958; Pyramichlamys cordiformis (H.J.Carter) H.Ettl & O.Ettl 1959  
Former name  
Common name  
Locality (Date of collection) Shimokubo Dam Oniishi Gunma Japan (1980-04-**)  
Latitude / Longitude 36.131497 / 139.007349 
Habitat (Isolation source) Freshwater (Lake water)  
History < Watanabe, Makoto M.  
Isolator (Date of isolation) Watanabe, Makoto M. (1980-04-**)  
Identified by Inouye, Isao  
State of strain Subculture; Unialgal; Clonal; Axenic[2018 Jan]  
Culture condition
(Preculture condition)
Medium:  C  
Temperature:  20 C
Light intensity:  24 µmol photons/m2/sec, L/D cycle:  10L:14D
Duration:  42 D  
Gene information  
Cell size (min - max) 12 - 20 μm  
Organization Unicellular; Flagellate 
Characteristics Other water bloom ; Starch production (Shimonaga, T. 2008)  
Other strain no. Other strain no. : SM-6-9  
Remarks Axenic 
Movie  
Reference
Hamana, K., Kobayashi, M., Furuchi, T., Hayashi, H., Niitsu, M. 2018 Polyamine analysis of unicellular, colonial, and multicellular green algae —Detection of aminobutylcadaverine, N1-aminopentylspermidine, N8-aminopentylspermidine, and penta-amines.— Microb. Resour. Syst., 34, 73-82.
Keywords: aminobutylcadaverine; aminopentylspermidine; green alga; penta-amine; polyamine; thermospermine
Strain(s): 1835242691735127112782185218722352261254025723983 

Hamana, K., Niitsu, M., Hayashi, H. 2013 Occurrence of homospermidine and thermospermine as a cellular polyamine in unicellular chlorophyte and multicellular charophyte green algae J. Gen. Appl. Micobiol., 59, 313-319.
Keywords: green alga; homospermidine; polyamine; thermospermine
Strain(s): 1815116025529630636036364072283614091410141114191837216421993374 
PubMed: 24005181
DOI: 10.2323/jgam.59.313

Shimonaga, T., Konishi, M., Oyama, Y., Fujiwara, S., Satoh, A., Fujita, N., Colleoni, C., Buléon, A., Putaux, J.-L., Ball, S. G., Yokoyma, A., Hara, Y., Nakamura, Y., Tsuzuki, M. 2008 Variation in Storage α-polyglucans of the Porphyridiales (Rhodophyta). Plant Cell Physiol., 49, 103-116.
Keywords: Amylose; Floridean starch; Glycogen; Porphyridiales; Rhodophyta; Semi-amylopection
Strain(s): 181442505305512138216022582280 
PubMed: 18079144
DOI: 10.1093/pcp/pcm172

Hamana, K., Aizaki T., Arai, E., Saito, A., Uchikata, K., Ohnishi, H. 2004 Distribution of norspermidine s a cellular polyamine within micro green algae including non-photosynthetic achlorophyllous Polytoma, Polytomella, Prototheca and Helicosporidium. J. Gen. Appl. Microbiol., 50, 289-295.
Keywords: Chlorophyta; green alga; Helicosporidium; norspermidine; polyamine; Polytoma; Polytomella; Prototheca
Strain(s): 18251254255296329483486536 

Watanabe, M. M., Kasai, F., Hiwatari, T., Suda, S., Nei, T. 1984 Cryopreservation of microalgae in liquid nitrogen - Survival of algal cultures after freezing. Jpn. J. Freez. Dry., 30, 23-26 (in Japanese with English title).
Strain(s): 1819212426293139404144454650737475767780818788899091939598991001011201221311351371441512042072132572633794152219223522392579 

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