Strain Data

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Strain number		NIES-223
Phylum		Heterokontophyta
Class		Bacillariophyceae
Scientific name		Skeletonema marinoi-dohrnii complex
Synonym
Former name		Skeletonema costatum (Greville) Cleve
Common name		Centric diatom
Locality (Date of collection)		Shodo Isl., Kagawa, Japan (1979-07-12)
Latitude / Longitude
Habitat (Isolation source)		Marine
History		< KAGAWA
Isolator (Date of isolation)		Yuki, Katsuhisa
Identified by
State of strain		Subculture; Unialgal; Clonal; Non-axenic
Culture condition (Preculture condition)		Medium: f/2 Temperature: 15 C Light intensity: 8 µmol photons/m²/sec, L/D cycle: 10L:14D Duration: 1 M
Gene information		18S rRNA ( AB488609 ) , 28S rRNA ( DQ396507 ) , CO1 ( AB706229 )
Cell size (min - max)		- 5 μm
Organization		Unicellular
Characteristics		Red tide ; Planktonic ; DNA sequencing indicates this strain is included in Skeletonema marinoi/dohrnii clade
Other strain no.		Other strain no. : KGW-26
Remarks
Movie

Reference
Yamada, M., Otsubo, M., Tsutsumi, Y., Mizota, C., Nakamura, Y., Takahashi, K., Iwataki M. 2017 Utility of mitochondrial-encoded cytochrome c oxidase I gene for phylogenetic analysis and species identification of the planktonic diatom genus Skeletonema. Phycol. Res., 65, 217-225. Keywords: cox1; genetic marker; LSU rDNA; phylogenetictree; species delimitation; SSU rDNA Strain(s): 16, 223, 2837, 2838, 2842 DOI: 10.1111/pre.12179 Mitani, E., Nakayama, F., Matsuwaki, I., Ichi, I., Kawabata, A., Kawachi, M., Kato, M. 2017 Fatty acid composition profiles of 235 strains of three microalgal divisions within the NIES Microbial Culture Collection. Microb. Resour. Syst., 33, 19-29. Keywords: Cryptophyta; docosahexaenoic acid; eicosapentaenoic acid; fatty acid; Haptophyta; Heterokontophyta; microalgae Strain(s): 1, 8, 9, 14, 15, 17, 71, 115, 223, 225, 233, 234, 265, 274, 275, 276, 277, 278, 279, 280, 281, 282, 284, 293, 323, 324, 330, 333, 344, 345, 346, 347, 348, 350, 353, 372, 377, 388, 391, 395, 407, 408, 409, 413, 414, 417, 461, 462, 466, 487, 534, 548, 553, 556, 557, 558, 559, 560, 562, 587, 588, 589, 590, 603, 605, 622, 623, 695, 696, 697, 698, 699, 700, 701, 702, 703, 704, 705, 706, 707, 708, 709, 710, 711, 712, 713, 714, 715, 716, 741, 765, 766, 767, 805, 837, 997, 1001, 1002, 1003, 1004, 1005, 1006, 1007, 1009, 1011, 1016, 1017, 1044, 1045, 1046, 1047, 1302, 1303, 1324, 1327, 1330, 1339, 1340, 1349, 1353, 1370, 1375, 1376, 1379, 1383, 1384, 1385, 1386, 1387, 1391, 1392, 1393, 1395, 1398, 1399, 1400, 1401, 1699, 1700, 1730, 1813, 1815, 1816, 1826, 1827, 1831, 1862, 1863, 1864, 1865, 1874, 1963, 1964, 1965, 1974, 1975, 1976, 2142, 2143, 2144, 2145, 2147, 2148, 2300, 2331, 2332, 2351, 2363, 2364, 2365, 2369, 2370, 2376, 2506, 2533, 2534, 2535, 2536, 2537, 2590, 2633, 2668, 2689, 2690, 2691, 2693, 2694, 2696, 2697, 2707, 2716, 2717, 2718, 2720, 2722, 2723, 2725, 2726, 2729, 2730, 2731, 2732, 2770, 2771, 2772, 2773, 2839, 2840, 2841, 2842, 2843, 2844, 2859, 2872, 2878, 2890, 2899, 3391, 3689, 3690, 3691 Kooistra, W. H. C. F., Sarno, D., Balzano, S., Gu, H., Andersen, R. A., Zingone, A. 2008 Global diversity and biogeography of Skeletonema species (Bacillariophyta). Protist, 159, 177-193. Keywords: biogeography; cryptic species; distribution; LSU rDNA; phytoplankton; Skeletonema costatum Strain(s): 16, 17, 223, 323, 324 PubMed: 18042429 DOI: 10.1016/j.protis.2007.09.004

Reference

Yamada, M., Otsubo, M., Tsutsumi, Y., Mizota, C., Nakamura, Y., Takahashi, K., Iwataki M. 2017 Utility of mitochondrial-encoded cytochrome c oxidase I gene for phylogenetic analysis and species identification of the planktonic diatom genus Skeletonema. Phycol. Res., 65, 217-225.
Keywords: cox1; genetic marker; LSU rDNA; phylogenetictree; species delimitation; SSU rDNA
Strain(s): 16, 223, 2837, 2838, 2842
DOI: 10.1111/pre.12179

Mitani, E., Nakayama, F., Matsuwaki, I., Ichi, I., Kawabata, A., Kawachi, M., Kato, M. 2017 Fatty acid composition profiles of 235 strains of three microalgal divisions within the NIES Microbial Culture Collection. Microb. Resour. Syst., 33, 19-29.
Keywords: Cryptophyta; docosahexaenoic acid; eicosapentaenoic acid; fatty acid; Haptophyta; Heterokontophyta; microalgae
Strain(s): 1, 8, 9, 14, 15, 17, 71, 115, 223, 225, 233, 234, 265, 274, 275, 276, 277, 278, 279, 280, 281, 282, 284, 293, 323, 324, 330, 333, 344, 345, 346, 347, 348, 350, 353, 372, 377, 388, 391, 395, 407, 408, 409, 413, 414, 417, 461, 462, 466, 487, 534, 548, 553, 556, 557, 558, 559, 560, 562, 587, 588, 589, 590, 603, 605, 622, 623, 695, 696, 697, 698, 699, 700, 701, 702, 703, 704, 705, 706, 707, 708, 709, 710, 711, 712, 713, 714, 715, 716, 741, 765, 766, 767, 805, 837, 997, 1001, 1002, 1003, 1004, 1005, 1006, 1007, 1009, 1011, 1016, 1017, 1044, 1045, 1046, 1047, 1302, 1303, 1324, 1327, 1330, 1339, 1340, 1349, 1353, 1370, 1375, 1376, 1379, 1383, 1384, 1385, 1386, 1387, 1391, 1392, 1393, 1395, 1398, 1399, 1400, 1401, 1699, 1700, 1730, 1813, 1815, 1816, 1826, 1827, 1831, 1862, 1863, 1864, 1865, 1874, 1963, 1964, 1965, 1974, 1975, 1976, 2142, 2143, 2144, 2145, 2147, 2148, 2300, 2331, 2332, 2351, 2363, 2364, 2365, 2369, 2370, 2376, 2506, 2533, 2534, 2535, 2536, 2537, 2590, 2633, 2668, 2689, 2690, 2691, 2693, 2694, 2696, 2697, 2707, 2716, 2717, 2718, 2720, 2722, 2723, 2725, 2726, 2729, 2730, 2731, 2732, 2770, 2771, 2772, 2773, 2839, 2840, 2841, 2842, 2843, 2844, 2859, 2872, 2878, 2890, 2899, 3391, 3689, 3690, 3691

Kooistra, W. H. C. F., Sarno, D., Balzano, S., Gu, H., Andersen, R. A., Zingone, A. 2008 Global diversity and biogeography of Skeletonema species (Bacillariophyta). Protist, 159, 177-193.
Keywords: biogeography; cryptic species; distribution; LSU rDNA; phytoplankton; Skeletonema costatum
Strain(s): 16, 17, 223, 323, 324
PubMed: 18042429
DOI: 10.1016/j.protis.2007.09.004

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